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Rabbit Anti-GRAP Recombinant Antibody (EG1428) (CBMAB-EN1707-LY)

The product is antibody recognizes GRAP. The antibody EG1428 immunoassay techniques such as: WB: 1:500~1:1000 ELISA: 1:5000.
See all GRAP antibodies

Summary

Host Animal
Rabbit
Specificity
Human, Mouse, Rat
Clone
EG1428
Antibody Isotype
IgG
Application
WB: 1:500~1:1000 ELISA: 1:5000

Basic Information

Immunogen
The antibody was produced against synthesized peptide derived from internal of human GRAP.
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
GRB2 related adaptor protein
Introduction
This gene encodes a member of the GRB2/Sem5/Drk family and functions as a cytoplasmic signaling protein which contains an SH2 domain flanked by two SH3 domains. The SH2 domain interacts with ligand-activated receptors for stem cell factor and erythropoietin, and facilitates the formation of a stable complex with the BCR-ABL oncoprotein. This protein also associates with the Ras guanine nucleotide exchange factor SOS1 (son of sevenless homolog 1) through its N-terminal SH3 domain. In general, it couples signals from receptor and cytoplasmic tyrosine kinases to the Ras signaling pathway.
Entrez Gene ID
Human10750
Mouse71520
Rat363616
UniProt ID
HumanQ13588
MouseQ9CX99
RatQ4KM68
Alternative Names
DFNB114
Function
Couples signals from receptor and cytoplasmic tyrosine kinases to the Ras signaling pathway. Plays a role in the inner ear and in hearing (PubMed:30610177).
Biological Process
Cell-cell signaling Source: ProtInc
Ras protein signal transduction Source: ProtInc
Sensory perception of sound Source: UniProtKB
Cellular Location
Membrane; Synapse. Localizes at the presynaptic terminal.
Involvement in disease
Deafness, autosomal recessive, 114 (DFNB114):
A form of non-syndromic deafness characterized by congenital profound sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.

Nocka, L. M., Eisen, T. J., Iavarone, A. T., Groves, J. T., & Kuriyan, J. (2023). Stimulation of the catalytic activity of the tyrosine kinase Btk by the adaptor protein Grb2. Elife, 12, e82676.

Ruminski, K., Celis-Gutierrez, J., Jarmuzynski, N., Maturin, E., Audebert, S., Malissen, M., ... & Roncagalli, R. (2023). Mapping the SLP76 interactome in T cells lacking each of the GRB2-family adaptors reveals molecular plasticity of the TCR signaling pathway. Frontiers in Immunology, 14.

Lin, C. C., Wieteska, L., Suen, K. M., Kalverda, A. P., Ahmed, Z., & Ladbury, J. E. (2021). Grb2 binding induces phosphorylation-independent activation of Shp2. Communications biology, 4(1), 437.

Vögtle, T., Baig, A. A., Volz, J., Duchow, T. B., Pleines, I., Dütting, S., ... & Nieswandt, B. (2020). Critical redundant functions of the adapters Grb2 and Gads in platelet (hem) ITAM signaling in mice. Platelets, 31(6), 801-811.

Nishino, T., Tamada, K., Maeda, A., Abe, T., Kiyonari, H., Funahashi, Y., ... & Konishi, H. (2019). Behavioral analysis in mice deficient for GAREM2 (Grb2-associated regulator of Erk/MAPK subtype2) that is a subtype of highly expressing in the brain. Molecular brain, 12, 1-13.

Yablonski, D. (2019). Bridging the gap: modulatory roles of the Grb2-family adaptor, Gads, in cellular and allergic immune responses. Frontiers in Immunology, 10, 1704.

Li, C., Bademci, G., Subasioglu, A., Diaz-Horta, O., Zhu, Y., Liu, J., ... & Tekin, M. (2019). Dysfunction of GRAP, encoding the GRB2-related adaptor protein, is linked to sensorineural hearing loss. Proceedings of the National Academy of Sciences, 116(4), 1347-1352.

Lu, M., Wang, C., Chen, W., Mao, C., & Wang, J. (2018). miR-654-5p targets GRAP to promote proliferation, metastasis, and chemoresistance of oral squamous cell carcinoma through Ras/MAPK signaling. DNA and cell biology, 37(4), 381-388.

Vanshylla, K., Bartsch, C., Hitzing, C., Krümpelmann, L., Wienands, J., & Engels, N. (2018). Grb2 and GRAP connect the B cell antigen receptor to Erk MAP kinase activation in human B cells. Scientific reports, 8(1), 4244.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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