Sign in or Register   Sign in or Register
  |  

Mouse Anti-HACE1 Recombinant Antibody (CBFYH-0650) (CBMAB-H0054-FY)

This product is mouse antibody that recognizes HACE1. The antibody CBFYH-0650 can be used for immunoassay techniques such as: ELISA, IF, IHC-P, WB.
See all HACE1 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBFYH-0650
Antibody Isotype
IgG1, κ
Application
ELISA, IF, IHC-P, WB

Basic Information

Immunogen
Mouse monoclonal antibodies raised against His-tagged recombinant full length Human HACE1
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Concentration
1 mg/mL
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1
Introduction
This gene encodes a HECT domain and ankyrin repeat-containing ubiquitin ligase. The encoded protein is involved in specific tagging of target proteins, leading to their subcellular localization or proteasomal degradation. The protein is a potential tumor suppressor and is involved in the pathophysiology of several tumors, including Wilm's tumor.
Entrez Gene ID
57531
UniProt ID
Q8IYU2
Alternative Names
HECT Domain And Ankyrin Repeat Containing E3 Ubiquitin Protein Ligase 1; HECT-Type E3 Ubiquitin Transferase HACE1; HECT Domain And Ankyrin Repeat-Containing E3 Ubiquitin-Protein Ligase 1; E3 Ubiquitin-Protein Ligase HACE1; EC 2.3.2.26; KIAA1320; EC 6.3.2; SPPRS
Function
E3 ubiquitin-protein ligase involved in Golgi membrane fusion and regulation of small GTPases. Acts as a regulator of Golgi membrane dynamics during the cell cycle: recruited to Golgi membrane by Rab proteins and regulates postmitotic Golgi membrane fusion. Acts by mediating ubiquitination during mitotic Golgi disassembly, ubiquitination serving as a signal for Golgi reassembly later, after cell division. Specifically interacts with GTP-bound RAC1, mediating ubiquitination and subsequent degradation of active RAC1, thereby playing a role in host defense against pathogens. May also act as a transcription regulator via its interaction with RARB.
Biological Process
Cell cycle Source: UniProtKB-KW
Golgi organization Source: UniProtKB
Membrane fusion Source: UniProtKB
Positive regulation of protein catabolic process Source: GO_Central
Proteasome-mediated ubiquitin-dependent protein catabolic process Source: GO_Central
Protein K48-linked ubiquitination Source: UniProtKB
Protein polyubiquitination Source: GO_Central
Protein ubiquitination Source: UniProtKB
Rac protein signal transduction Source: UniProtKB
Regulation of cell migration Source: UniProtKB
Ubiquitin-dependent protein catabolic process Source: UniProtKB
Cellular Location
Golgi stack membrane; Endoplasmic reticulum; Cytoplasm. A significant portion localizes to the endoplasmic reticulum. Targeted to Golgi membrane via its interaction with Rab proteins.
Involvement in disease
Defects in HACE1 are a cause of Wilms tumor (WT). WT is a pediatric malignancy of kidney and one of the most common solid cancers in childhood. HACE1 is epigenetically down-regulated in sporadic Wilms tumor. Moreover, a t(5;6)(q21;q21) translocation that truncates HACE1 has been found in a child with bilateral, young-onset Wilms tumor (PubMed:19948536).
Spastic paraplegia and psychomotor retardation with or without seizures (SPPRS):
A form of spastic paraplegia, a neurodegenerative disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs. Rate of progression and the severity of symptoms are quite variable. Initial symptoms may include difficulty with balance, weakness and stiffness in the legs, muscle spasms, and dragging the toes when walking. In some forms of the disorder, bladder symptoms (such as incontinence) may appear, or the weakness and stiffness may spread to other parts of the body. SPPRS is an autosomal recessive neurodevelopmental disorder manifesting in infancy. Affected individuals show hypotonia and psychomotor retardation. Most develop seizures.

Zang, C. X., Wang, L., Yang, H. Y., Shang, J. M., Liu, H., Zhang, Z. H., ... & Zhang, D. (2022). HACE1 negatively regulates neuroinflammation through ubiquitylating and degrading Rac1 in Parkinson’s disease models. Acta Pharmacologica Sinica, 43(2), 285-294.

Jiang, H. Y., Chen, Y. L., Xu, X. X., Li, C. Y., Chen, Y., Li, D. P., ... & Gao, H. (2022). Ubiquitylation of cyclin C by HACE1 regulates cisplatin‐associated sensitivity in gastric cancer. Clinical and Translational Medicine, 12(3), e770.

Da, C., Pu, J., Liu, Z., Wei, J., Qu, Y., Wu, Y., ... & Hou, P. (2021). HACE1-mediated NRF2 activation causes enhanced malignant phenotypes and decreased radiosensitivity of glioma cells. Signal Transduction and Targeted Therapy, 6(1), 399.

Kogler, M., Tortola, L., Negri, G. L., Leopoldi, A., El-Naggar, A. M., Mereiter, S., ... & Penninger, J. M. (2020). HACE1 prevents lung carcinogenesis via inhibition of RAC-family GTPases. Cancer research, 80(14), 3009-3022.

Zhou, Z., Zhang, H. S., Zhang, Z. G., Sun, H. L., Liu, H. Y., Gou, X. M., ... & Huang, Y. H. (2019). Loss of HACE1 promotes colorectal cancer cell migration via upregulation of YAP1. Journal of Cellular Physiology, 234(6), 9663-9672.

Li, J. C., Chang, X., Chen, Y., Li, X. Z., Zhang, X. L., Yang, S. M., ... & Zhang, H. (2019). Loss of the tumor suppressor HACE1 contributes to cancer progression. Current Drug Targets, 20(10), 1018-1028.

El-Naggar, A. M., Clarkson, P. W., Negri, G. L., Turgu, B., Zhang, F., Anglesio, M. S., & Sorensen, P. H. (2019). HACE1 is a potential tumor suppressor in osteosarcoma. Cell death & disease, 10(1), 21.

Nagy, V., Hollstein, R., Pai, T. P., Herde, M. K., Buphamalai, P., Moeseneder, P., ... & Penninger, J. M. (2019). HACE1 deficiency leads to structural and functional neurodevelopmental defects. Neurology Genetics, 5(3).

El-Hachem, N., Habel, N., Naiken, T., Bzioueche, H., Cheli, Y., Beranger, G. E., ... & Ballotti, R. (2018). Uncovering and deciphering the pro-invasive role of HACE1 in melanoma cells. Cell Death & Differentiation, 25(11), 2010-2022.

Ehrnhoefer, D. E., Southwell, A. L., Sivasubramanian, M., Qiu, X., Villanueva, E. B., Xie, Y., ... & Hayden, M. R. (2018). HACE1 is essential for astrocyte mitochondrial function and influences Huntington disease phenotypes in vivo. Human molecular genetics, 27(2), 239-253.

Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Mouse Anti-HACE1 Recombinant Antibody (CBFYH-0650)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Documents

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Go to
Compare