Mouse Anti-IRAK1 Recombinant Antibody (3A9) (CBMAB-A4510-LY)

Mouse

Human

3A9

IgG2a, κ

WB, ELISA

IRAK1 (AAH54000, 530 a.a. ~ 693 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Human

IgG2a, κ

Monoclonal

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Liquid

> 95% Purity determined by SDS-PAGE.

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Interleukin 1 Receptor Associated Kinase 1

This gene encodes the interleukin-1 receptor-associated kinase 1, one of two putative serine/threonine kinases that become associated with the interleukin-1 receptor (IL1R) upon stimulation. This gene is partially responsible for IL1-induced upregulation of the transcription factor NF-kappa B. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

IRAK; pelle

Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways. Is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation. Association with MYD88 leads to IRAK1 phosphorylation by IRAK4 and subsequent autophosphorylation and kinase activation. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates the interferon regulatory factor 7 (IRF7) to induce its activation and translocation to the nucleus, resulting in transcriptional activation of type I IFN genes, which drive the cell in an antiviral state. When sumoylated, translocates to the nucleus and phosphorylates STAT3.

Activation of NF-kappaB-inducing kinase activityManual Assertion Based On ExperimentIDA:UniProtKB
AgingIEA:Ensembl
Cellular response to heatIEA:Ensembl
Cellular response to hypoxiaIEA:Ensembl
Cellular response to lipopolysaccharideManual Assertion Based On ExperimentIBA:GO_Central
Cytokine-mediated signaling pathwayManual Assertion Based On ExperimentIBA:GO_Central
Innate immune responseManual Assertion Based On ExperimentIBA:GO_Central
Interleukin-1-mediated signaling pathwayManual Assertion Based On ExperimentIMP:BHF-UCL
Intracellular signal transductionManual Assertion Based On ExperimentIBA:GO_Central
JNK cascadeIEA:Ensembl
Lipopolysaccharide-mediated signaling pathwayManual Assertion Based On ExperimentIMP:BHF-UCL
MyD88-dependent toll-like receptor signaling pathwayManual Assertion Based On ExperimentTAS:BHF-UCL
Negative regulation of NF-kappaB transcription factor activityManual Assertion Based On ExperimentIMP:BHF-UCL
Positive regulation of I-kappaB kinase/NF-kappaB signalingManual Assertion Based On ExperimentIMP:MGI
Positive regulation of leukocyte adhesion to vascular endothelial cellManual Assertion Based On ExperimentIMP:ARUK-UCL
Positive regulation of MAP kinase activityManual Assertion Based On ExperimentIBA:GO_Central
Positive regulation of NF-kappaB transcription factor activityManual Assertion Based On ExperimentIDA:BHF-UCL
Positive regulation of NIK/NF-kappaB signalingManual Assertion Based On ExperimentIMP:ARUK-UCL
Positive regulation of smooth muscle cell proliferationIEA:Ensembl
Positive regulation of type I interferon productionManual Assertion Based On ExperimentIMP:BHF-UCL
Protein autophosphorylationManual Assertion Based On ExperimentIDA:BHF-UCL
Protein phosphorylationManual Assertion Based On ExperimentTAS:ProtInc
Regulation of cytokine-mediated signaling pathwayManual Assertion Based On ExperimentIMP:BHF-UCL
Response to interleukin-1Manual Assertion Based On ExperimentIMP:BHF-UCL
Response to lipopolysaccharideManual Assertion Based On ExperimentIMP:BHF-UCL
Toll-like receptor 2 signaling pathwayManual Assertion Based On ExperimentIMP:BHF-UCL
Toll-like receptor 4 signaling pathwayManual Assertion Based On ExperimentIBA:GO_Central
Toll-like receptor 9 signaling pathwayTAS:Reactome
Toll-like receptor signaling pathwayTAS:Reactome
Type I interferon signaling pathwayManual Assertion Based On ExperimentIMP:BHF-UCL

Cytoplasm; Nucleus; Lipid droplet. Translocates to the nucleus when sumoylated. RSAD2/viperin recruits it to the lipid droplet (By similarity).

Following recruitment on the activated receptor complex, phosphorylated on Thr-209, probably by IRAK4, resulting in a conformational change of the kinase domain, allowing further phosphorylations to take place. Thr-387 phosphorylation in the activation loop is required to achieve full enzymatic activity.
Polyubiquitinated by TRAF6 after cell stimulation with IL-1-beta by PELI1, PELI2 and PELI3. Polyubiquitination occurs with polyubiquitin chains linked through 'Lys-63'. Ubiquitination promotes interaction with NEMO/IKBKG. Also sumoylated; leading to nuclear translocation.