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Mouse Anti-KANK1 Recombinant Antibody (2B8) (CBMAB-A4528-YC)

Provided herein is a Mouse monoclonal antibody against Human KN Motif And Ankyrin Repeat Domains 1. The antibody can be used for immunoassay techniques, such as ELISA, WB.
See all KANK1 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
2B8
Antibody Isotype
IgG2a, κ
Application
ELISA, WB

Basic Information

Immunogen
Partial recombinant corresponding to aa701-800 from ANKRD15 (AAH38116) with GST tag.
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid in PBS, pH 7.2
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
KN Motif And Ankyrin Repeat Domains 1
Introduction
KANK1 belongs to the Kank family of proteins, which contain multiple ankyrin repeat domains. This family member functions in cytoskeleton formation by regulating actin polymerization. This gene is a candidate tumor suppressor for renal cell carcinoma. Mut
Entrez Gene ID
23189
UniProt ID
Q14678
Alternative Names
KN Motif And Ankyrin Repeat Domains 1; Ankyrin Repeat Domain-Containing Protein 15; Kidney Ankyrin Repeat-Containing Protein; ANKRD15; KANK; KN Motif And Ankyrin Repeat Domain-Containing Protein 1; Ankyrin Repeat Domain 15; KIAA0172; CPSQ2;
Function
Involved in the control of cytoskeleton formation by regulating actin polymerization. Inhibits actin fiber formation and cell migration (PubMed:25961457).
Inhibits RhoA activity; the function involves phosphorylation through PI3K/Akt signaling and may depend on the competetive interaction with 14-3-3 adapter proteins to sequester them from active complexes (PubMed:25961457).
Inhibits the formation of lamellipodia but not of filopodia; the function may depend on the competetive interaction with BAIAP2 to block its association with activated RAC1 (PubMed:25961457).
Inhibits fibronectin-mediated cell spreading; the function is partially mediated by BAIAP2. Inhibits neurite outgrowth. Involved in the establishment and persistence of cell polarity during directed cell movement in wound healing. In the nucleus, is involved in beta-catenin-dependent activation of transcription. Potential tumor suppressor for renal cell carcinoma. Regulates Rac signaling pathways (PubMed:25961457).
Biological Process
Actin cytoskeleton organizationManual Assertion Based On ExperimentIMP:UniProtKB
Cell population proliferationManual Assertion Based On ExperimentIMP:UniProtKB
Glomerular visceral epithelial cell migrationManual Assertion Based On ExperimentIMP:UniProtKB
Negative regulation of actin filament polymerizationManual Assertion Based On ExperimentIDA:UniProtKB
Negative regulation of cell migrationManual Assertion Based On ExperimentIMP:UniProtKB
Negative regulation of insulin receptor signaling pathwayManual Assertion Based On ExperimentIMP:UniProtKB
Negative regulation of lamellipodium morphogenesisManual Assertion Based On ExperimentIDA:UniProtKB
Negative regulation of neuron projection developmentManual Assertion Based On ExperimentIDA:UniProtKB
Negative regulation of Rho protein signal transductionManual Assertion Based On ExperimentIMP:UniProtKB
Negative regulation of ruffle assemblyManual Assertion Based On ExperimentIDA:UniProtKB
Negative regulation of substrate adhesion-dependent cell spreadingManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of canonical Wnt signaling pathwayManual Assertion Based On ExperimentIMP:UniProtKB
Positive regulation of Wnt signaling pathwayManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of wound healingManual Assertion Based On ExperimentIMP:UniProtKB
Regulation of establishment of cell polarityManual Assertion Based On ExperimentIMP:UniProtKB
Regulation of Rho protein signal transductionManual Assertion Based On ExperimentIMP:UniProtKB
Cellular Location
Cell projection, ruffle membrane; Cytoplasm; Nucleus. Colocalizes with KIF21A in membrane ruffles (PubMed:19559006).
Shuttles between the cytoplasm and nucleus (PubMed:16968744).
Isoform 1&2: Cytoplasm; Nucleus. Shuttles between the cytoplasm and nucleus.
Involvement in disease
Cerebral palsy, spastic quadriplegic 2 (CPSQ2):
A non-progressive disorder of movement and/or posture resulting from defects in the developing central nervous system. Affected individuals manifest congenital hypotonia evolving over the first year to spastic quadriplegia with accompanying transient nystagmus and varying degrees of mental retardation. Neuroimaging shows brain atrophy and ventriculomegaly.

Guo, K., Zhang, J., Huang, P., Xu, Y., Pan, W., Li, K., ... & Yu, C. (2023). KANK1 shapes focal adhesions by orchestrating protein binding, mechanical force sensing, and phase separation. Cell Reports, 42(11).

Nguyen, M. T., & Lee, W. (2022). Kank1 Is Essential for Myogenic Differentiation by Regulating Actin Remodeling and Cell Proliferation in C2C12 Progenitor Cells. Cells, 11(13), 2030.

Li, M., Wu, W., Deng, S., Shao, Z., & Jin, X. (2021). TRAIP modulates the IGFBP3/AKT pathway to enhance the invasion and proliferation of osteosarcoma by promoting KANK1 degradation. Cell Death & Disease, 12(8), 767.

Wu, K. X., Wang, X. T., Hu, X. L., Jiang, X. Y., Zhuang, J. C., Xu, Y. Z., ... & Liu, L. L. (2021). LncRNA-ENST00000421645 upregulates kank1 to inhibit IFN-γ expression and promote T cell apoptosis in neurosyphilis. Frontiers in Microbiology, 12, 749171.

Pu, J., Shen, J., Zhong, Z., Yanling, M., & Gao, J. (2020). KANK1 regulates paclitaxel resistance in lung adenocarcinoma A549 cells. Artificial Cells, Nanomedicine, and Biotechnology, 48(1), 639-647.

Fan, H., Tian, H., Cheng, X., Chen, Y., Liang, S., Zhang, Z., ... & Xu, P. (2020). Aberrant Kank1 expression regulates YAP to promote apoptosis and inhibit proliferation in OSCC. Journal of Cellular Physiology, 235(2), 1850-1865.

Vanzo, R. J., Twede, H., Ho, K. S., Prasad, A., Martin, M. M., South, S. T., & Wassman, E. R. (2019). Clinical significance of copy number variants involving KANK1 in patients with neurodevelopmental disorders. European journal of medical genetics, 62(1), 15-20.

Yu, M., Le, S., Ammon, Y. C., Goult, B. T., Akhmanova, A., & Yan, J. (2019). Force-dependent regulation of talin–KANK1 complex at focal adhesions. Nano letters, 19(9), 5982-5990.

Gu, Y., & Zhang, M. (2018). Upregulation of the Kank1 gene inhibits human lung cancer progression in vitro and in vivo. Oncology Reports, 40(3), 1243-1250.

Weng, Z., Shang, Y., Yao, D., Zhu, J., & Zhang, R. (2018). Structural analyses of key features in the KANK1· KIF21A complex yield mechanistic insights into the cross-talk between microtubules and the cell cortex. Journal of Biological Chemistry, 293(1), 215-225.

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For research use only. Not intended for any clinical use.

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