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Mouse Anti-KCNA5 Recombinant Antibody (S7-45) (CBMAB-K0484-LY)

This product is antibody recognizes KCNA5. The antibody S7-45 immunoassay techniques such as: ICC/IF, IHC, IP, WB.
See all KCNA5 antibodies
Published Data
Fig.1 Western blot analysis of the expression of Kv1.5 subunits.
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Summary

Host Animal
Mouse
Specificity
Rat, Human, Mouse
Clone
S7-45
Antibody Isotype
IgG1
Application
ICC/IF, IHC, IP, WB

Basic Information

Immunogen
Fusion protein amino acids 542-602 (Cytoplasmic C-terminus) of rat Kv1.5
Specificity
Rat, Human, Mouse
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
50% glycerol
Concentration
1 mg/mL
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
potassium voltage-gated channel subfamily A member 5
Introduction
Potassium channels represent the most complex class of voltage-gated ino channels from both functional and structural standpoints. Their diverse functions include regulating neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume. Four sequence-related potassium channel genes - shaker, shaw, shab, and shal - have been identified in Drosophila, and each has been shown to have human homolog(s). This gene encodes a member of the potassium channel, voltage-gated, shaker-related subfamily. This member contains six membrane-spanning domains with a shaker-type repeat in the fourth segment. It belongs to the delayed rectifier class, the function of which could restore the resting membrane potential of beta cells after depolarization and thereby contribute to the regulation of insulin secretion. This gene is intronless, and the gene is clustered with genes KCNA1 and KCNA6 on chromosome 12. Defects in this gene are a cause of familial atrial fibrillation type 7 (ATFB7). [provided by RefSeq, May 2012]
Entrez Gene ID
Human3741
Mouse16493
Rat25470
UniProt ID
HumanP22460
MouseQ61762
RatP19024
Alternative Names
Kv1; Kv1.5
Function
Voltage-gated potassium channel that mediates transmembrane potassium transport in excitable membranes. Forms tetrameric potassium-selective channels through which potassium ions pass in accordance with their electrochemical gradient. The channel alternates between opened and closed conformations in response to the voltage difference across the membrane. Can form functional homotetrameric channels and heterotetrameric channels that contain variable proportions of KCNA1, KCNA2, KCNA4, KCNA5, and possibly other family members as well; channel properties depend on the type of alpha subunits that are part of the channel (PubMed:12130714).
Channel properties are modulated by cytoplasmic beta subunits that regulate the subcellular location of the alpha subunits and promote rapid inactivation (PubMed:12130714).
Homotetrameric channels display rapid activation and slow inactivation (PubMed:8505626, PubMed:12130714).
May play a role in regulating the secretion of insulin in normal pancreatic islets. Isoform 2 exhibits a voltage-dependent recovery from inactivation and an excessive cumulative inactivation (PubMed:11524461).
Biological Process
Atrial cardiac muscle cell action potentialManual Assertion Based On ExperimentIMP:BHF-UCL
Membrane hyperpolarizationManual Assertion Based On ExperimentIMP:BHF-UCL
Membrane repolarization during atrial cardiac muscle cell action potentialManual Assertion Based On ExperimentIMP:BHF-UCL
Membrane repolarization during bundle of His cell action potentialManual Assertion Based On ExperimentIMP:BHF-UCL
Membrane repolarization during SA node cell action potentialManual Assertion Based On ExperimentIMP:BHF-UCL
Negative regulation of cytosolic calcium ion concentrationIEA:Ensembl
Notch signaling pathwayIEA:Ensembl
Positive regulation of G1/S transition of mitotic cell cycleIEA:Ensembl
Positive regulation of myoblast proliferationIEA:Ensembl
Potassium ion export across plasma membraneManual Assertion Based On ExperimentIDA:BHF-UCL
Potassium ion homeostasisIEA:Ensembl
Potassium ion transmembrane transportManual Assertion Based On ExperimentIDA:UniProtKB
Potassium ion transportManual Assertion Based On ExperimentIDA:MGI
Protein homooligomerizationIEA:InterPro
Regulation of atrial cardiac muscle cell membrane repolarizationManual Assertion Based On ExperimentIMP:BHF-UCL
Regulation of heart rate by cardiac conductionManual Assertion Based On ExperimentIMP:BHF-UCL
Regulation of insulin secretionManual Assertion Based On ExperimentTAS:BHF-UCL
Regulation of membrane potentialManual Assertion Based On ExperimentIDA:BHF-UCL
Regulation of potassium ion transportManual Assertion Based On ExperimentIMP:BHF-UCL
Regulation of vasoconstrictionIEA:Ensembl
Response to hydrogen peroxideIEA:Ensembl
Response to hyperoxiaIEA:Ensembl
Response to hypoxiaIEA:Ensembl
Response to mechanical stimulusIEA:Ensembl
Response to organic substanceIEA:Ensembl
Cellular Location
Cell membrane
Involvement in disease
Atrial fibrillation, familial, 7 (ATFB7):
A familial form of atrial fibrillation, a common sustained cardiac rhythm disturbance. Atrial fibrillation is characterized by disorganized atrial electrical activity and ineffective atrial contraction promoting blood stasis in the atria and reduces ventricular filling. It can result in palpitations, syncope, thromboembolic stroke, and congestive heart failure.
Topology
Cytoplasmic: 1-247
Helical: 248-269
Extracellular: 270-323
Helical: 324-345
Cytoplasmic: 346-356
Helical: 357-377
Extracellular: 378-395
Helical: 396-416
Cytoplasmic: 417-431
Helical: 432-453
Extracellular: 454-467
Helical: 468-487
Extracellular: 488-494
Helical: 495-523
Cytoplasmic: 524-613
PTM
Sumoylated on Lys-221, and Lys-536, preferentially with SUMO3. Sumoylation regulates the voltage sensitivity of the channel.

Vera-Zambrano, A., Lago-Docampo, M., Gallego, N., Franco-Gonzalez, J. F., Morales-Cano, D., Cruz-Utrilla, A., ... & Cogolludo, A. (2023). Novel loss-of-function KCNA5 variants in pulmonary arterial hypertension. American Journal of Respiratory Cell and Molecular Biology, 69(2), 147-158.

Wang, K., Zhao, J., & Guo, Z. (2022). Interaction of KCNA5, CX43, and CX40 proteins in the atrial muscle of patients with atrial fibrillation. Cell Biology International, 46(11), 1834-1840.

Cunningham, S. J., Barrera, A., Sankaranarayanan, L., Allen, T. K., & Reddy, T. E. (2022). Glucocorticoid Receptor Regulated KCNA5 Mediates Cell Proliferation in Human Fetal Membranes.

Zhou, S., Xia, L., Chen, Y., Guo, W., & Hu, J. (2021). miR-3188 Regulates proliferation and apoptosis of granulosa cells by targeting KCNA5 in the polycystic ovary syndrome. Acta Biochimica Polonica, 68(1), 83-89.

Farah, A., Kabbage, M., Atafi, S., Gabteni, A. J., Barbirou, M., Madhioub, M., ... & Bouhaouala-Zahar, B. (2020). Selective expression of KCNA5 and KCNB1 genes in gastric and colorectal carcinoma. BMC cancer, 20, 1-9.

Gao, L., Wu, D., Wu, Y., Yang, Z., Sheng, J., Lin, X., & Huang, H. (2020). MiR-3940-5p promotes granulosa cell proliferation through targeting KCNA5 in polycystic ovarian syndrome. Biochemical and Biophysical Research Communications, 524(4), 791-797.

Jiao, Y. R., Wang, W., Lei, P. C., Jia, H. P., Dong, J., Gou, Y. Q., ... & Zhu, Y. K. (2019). 5-HTT, BMPR2, EDN1, ENG, KCNA5 gene polymorphisms and susceptibility to pulmonary arterial hypertension: A meta-analysis. Gene, 680, 34-42.

Hauptman, N., Jevšinek Skok, D., Spasovska, E., Boštjančič, E., & Glavač, D. (2019). Genes CEP55, FOXD3, FOXF2, GNAO1, GRIA4, and KCNA5 as potential diagnostic biomarkers in colorectal cancer. BMC medical genomics, 12(1), 1-17.

Qu, C., Sun, J., Liu, Y., Wang, X., Wang, L., Han, C., ... & Liu, J. (2018). Caveolin‑1 facilitated KCNA5 expression, promoting breast cancer viability. Oncology letters, 16(4), 4829-4838.

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For research use only. Not intended for any clinical use.

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