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Rat Anti-KL Antibody (KL-115) (CBMAB-1368CQ)

This product is a rat antibody that recognizes KL. The antibody KL-115 can be used for immunoassay techniques such as: IHC-P, IF, IHC, IP, WB.
See all KL antibodies

Summary

Host Animal
Rat
Specificity
Human
Clone
KL-115
Antibody Isotype
IgG2a, κ
Application
IHC-P, IF, IHC, IP, WB

Basic Information

Immunogen
Recombinant peptide sequence-GST fusion (N-terminus of KL1 domain aa55-261)
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Supernatant
Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
aa 55-261

Target

Full Name
KLOTHO
Introduction
This gene encodes a type-I membrane protein that is related to beta-glucosidases. Reduced production of this protein has been observed in patients with chronic renal failure (CRF), and this may be one of the factors underlying the degenerative processes (e.g., arteriosclerosis, osteoporosis, and skin atrophy) seen in CRF. The Klotho peptide generated by cleavage of the membrane-bound isoform may be an anti-aging circulating hormone which would extend life span by inhibiting insulin/IGF1 signaling.
Entrez Gene ID
9365
UniProt ID
Q9UEF7
Alternative Names
KL; KLOTHO
Function
May have weak glycosidase activity towards glucuronylated steroids. However, it lacks essential active site Glu residues at positions 239 and 872, suggesting it may be inactive as a glycosidase in vivo. May be involved in the regulation of calcium and phosphorus homeostasis by inhibiting the synthesis of active vitamin D (By similarity).
Essential factor for the specific interaction between FGF23 and FGFR1 (By similarity).
The Klotho peptide generated by cleavage of the membrane-bound isoform may be an anti-aging circulating hormone which would extend life span by inhibiting insulin/IGF1 signaling.
Biological Process
Acute inflammatory responseIEA:Ensembl
AgingManual Assertion Based On ExperimentIMP:UniProtKB
Calcium ion homeostasisIEA:Ensembl
Carbohydrate metabolic processIEA:InterPro
Energy reserve metabolic processIEA:Ensembl
Fibroblast growth factor receptor signaling pathwayManual Assertion Based On ExperimentIBA:GO_Central
Insulin receptor signaling pathwayIEA:InterPro
Negative regulation of systemic arterial blood pressureIEA:Ensembl
Norepinephrine biosynthetic processIEA:Ensembl
Positive regulation of bone mineralizationManual Assertion Based On ExperimentIMP:UniProtKB
Positive regulation of MAPKKK cascade by fibroblast growth factor receptor signaling pathwayIEA:Ensembl
Response to activityIEA:Ensembl
Response to angiotensinIEA:Ensembl
Response to vitamin DIEA:Ensembl
Cellular Location
Isoform 1: Cell membrane; Apical cell membrane. Isoform 1 shedding leads to a soluble peptide.
Isoform 2: Secreted
Klotho peptide: Secreted

Abraham, C. R., & Li, A. (2022). Aging-suppressor Klotho: Prospects in diagnostics and therapeutics. Ageing Research Reviews, 101766.

Kresovich, J. K., & Bulka, C. M. (2022). Low serum klotho associated with all-cause mortality among a nationally representative sample of American adults. The Journals of Gerontology: Series A, 77(3), 452-456.

Chen, K., Wang, S., Sun, Q. W., Zhang, B., Ullah, M., & Sun, Z. (2021). Klotho deficiency causes heart aging via impairing the Nrf2-GR pathway. Circulation research, 128(4), 492-507.

Neyra, J. A., Hu, M. C., & Moe, O. W. (2021). Klotho in clinical nephrology: diagnostic and therapeutic implications. Clinical Journal of the American Society of Nephrology: CJASN, 16(1), 162.

Han, X., & Sun, Z. (2020). Epigenetic regulation of KL (Klotho) via H3K27me3 (histone 3 lysine [K] 27 trimethylation) in renal tubule cells. Hypertension, 75(5), 1233-1241.

Erickson, C. M., Schultz, S. A., Oh, J. M., Darst, B. F., Ma, Y., Norton, D., ... & Okonkwo, O. C. (2019). KLOTHO heterozygosity attenuates APOE4-related amyloid burden in preclinical AD. Neurology, 92(16), e1878-e1889.

Kuro-o, M. (2019). The Klotho proteins in health and disease. Nature Reviews Nephrology, 15(1), 27-44.

Tan, S. J., Chu, M. M., Toussaint, N. D., Cai, M. M., Hewitson, T. D., & Holt, S. G. (2018). High-intensity physical exercise increases serum α-klotho levels in healthy volunteers. Journal of circulating biomarkers, 7, 1849454418794582.

Xuan, N. T., & Van Hai, N. (2018). Changes in expression of klotho affect physiological processes, diseases, and cancer. Iranian journal of basic medical sciences, 21(1), 3.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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