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Mouse Anti-LPAR1 Recombinant Antibody (CBFYE-1495) (CBMAB-E2128-FY)

This product is mouse antibody that recognizes LPAR1. The antibody CBFYE-1495 can be used for immunoassay techniques such as: WB, IHC-P, FC.
See all LPAR1 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBFYE-1495
Antibody Isotype
IgG2b
Application
WB, IHC-P, FC

Basic Information

Specificity
Human
Antibody Isotype
IgG2b
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Concentration
0.76 mg/mL
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
lysophosphatidic acid receptor 1
Introduction
The integral membrane protein encoded by this gene is a lysophosphatidic acid (LPA) receptor from a group known as EDG receptors. These receptors are members of the G protein-coupled receptor superfamily.
Entrez Gene ID
UniProt ID
Alternative Names
EDG2; LPA1; VZG1; GPR26; edg-2; vzg-1; Gpcr26; Mrec1.3; rec.1.3
Function
Receptor for lysophosphatidic acid (LPA) (PubMed:9070858, PubMed:19306925, PubMed:25025571, PubMed:26091040).
Plays a role in the reorganization of the actin cytoskeleton, cell migration, differentiation and proliferation, and thereby contributes to the responses to tissue damage and infectious agents. Activates downstream signaling cascades via the G(i)/G(o), G(12)/G(13), and G(q) families of heteromeric G proteins. Signaling inhibits adenylyl cyclase activity and decreases cellular cAMP levels (PubMed:26091040).
Signaling triggers an increase of cytoplasmic Ca(2+) levels (PubMed:19656035, PubMed:19733258, PubMed:26091040).
Activates RALA; this leads to the activation of phospholipase C (PLC) and the formation of inositol 1,4,5-trisphosphate (PubMed:19306925).
Signaling mediates activation of down-stream MAP kinases (By similarity).
Contributes to the regulation of cell shape. Promotes Rho-dependent reorganization of the actin cytoskeleton in neuronal cells and neurite retraction (PubMed:26091040).
Promotes the activation of Rho and the formation of actin stress fibers (PubMed:26091040).
Promotes formation of lamellipodia at the leading edge of migrating cells via activation of RAC1 (By similarity).
Through its function as lysophosphatidic acid receptor, plays a role in chemotaxis and cell migration, including responses to injury and wounding (PubMed:18066075, PubMed:19656035, PubMed:19733258).
Plays a role in triggering inflammation in response to bacterial lipopolysaccharide (LPS) via its interaction with CD14. Promotes cell proliferation in response to lysophosphatidic acid. Required for normal skeleton development. May play a role in osteoblast differentiation. Required for normal brain development. Required for normal proliferation, survival and maturation of newly formed neurons in the adult dentate gyrus. Plays a role in pain perception and in the initiation of neuropathic pain (By similarity).
Biological Process
Activation of phospholipase C activityManual Assertion Based On ExperimentIDA:UniProtKB
Adenylate cyclase-activating G protein-coupled receptor signaling pathwayManual Assertion Based On ExperimentIBA:GO_Central
Adenylate cyclase-inhibiting G protein-coupled receptor signaling pathwayISS:UniProtKB
Cell chemotaxisManual Assertion Based On ExperimentIMP:UniProtKB
G protein-coupled receptor signaling pathwayManual Assertion Based On ExperimentTAS:ProtInc
Negative regulation of neuron projection developmentISS:UniProtKB
NeurogenesisManual Assertion Based On ExperimentIBA:GO_Central
Positive regulation of cytosolic calcium ion concentrationManual Assertion Based On ExperimentTAS:ProtInc
Positive regulation of cytosolic calcium ion concentration involved in phospholipase C-activating G protein-coupled signaling pathwayManual Assertion Based On ExperimentIMP:UniProtKB
Positive regulation of I-kappaB kinase/NF-kappaB signalingManual Assertion Based On ExperimentIEP:UniProtKB
Positive regulation of MAPK cascadeISS:UniProtKB
Positive regulation of Rho protein signal transductionISS:UniProtKB
Positive regulation of stress fiber assemblyISS:UniProtKB
Regulation of cell shapeISS:UniProtKB
Regulation of metabolic processManual Assertion Based On ExperimentIBA:GO_Central
Cellular Location
Cell surface
Cell membrane
Endosome
Prior to LPA treatment found predominantly at the cell surface. Internalized after LPA treatment. Colocalizes with RALA in endocytic vesicles after LPA treatment.
Topology
Extracellular: 1-50
Helical: 51-75
Cytoplasmic: 76-83
Helical: 84-107
Extracellular: 108-121
Helical: 122-144
Cytoplasmic: 145-163
Helical: 164-184
Extracellular: 185-204
Helical: 205-225
Cytoplasmic: 226-255
Helical: 256-280
Extracellular: 281-294
Helical: 295-315
Cytoplasmic: 316-364
PTM
N-glycosylated.

Chen, M., Shi, J. L., Zheng, Z. M., Lin, Z., Li, M. Q., & Shao, J. (2023). An abnormal LPA/LPAR1–NHE1 axis leads to the autophagy deficiency of trophoblast cells in recurrent spontaneous abortion. Reproduction, 166(5), 357-368.

Ahmadzai, M. M., McClain, J. L., Dharshika, C., Seguella, L., Giancola, F., De Giorgio, R., & Gulbransen, B. D. (2022). LPAR 1 regulates enteric nervous system function through glial signaling and contributes to chronic intestinal pseudo-obstruction. The Journal of Clinical Investigation, 132(4).

Liu, J., Rebecca, V. W., Kossenkov, A. V., Connelly, T., Liu, Q., Gutierrez, A., ... & Herlyn, M. (2021). Neural crest-like stem cell transcriptome analysis identifies LPAR1 in melanoma progression and therapy resistance. Cancer research, 81(20), 5230-5241.

Takagi, S., Sasaki, Y., Koike, S., Takemoto, A., Seto, Y., Haraguchi, M., ... & Katayama, R. (2021). Platelet-derived lysophosphatidic acid mediated LPAR1 activation as a therapeutic target for osteosarcoma metastasis. Oncogene, 40(36), 5548-5558.

Xiao, D., Su, X., Gao, H., Li, X., & Qu, Y. (2021). The roles of Lpar1 in central nervous system disorders and diseases. Frontiers in Neuroscience, 15, 710473.

Shi, J., Jiang, D., Yang, S., Zhang, X., Wang, J., Liu, Y., ... & Yang, K. (2020). LPAR1, correlated with immune infiltrates, is a potential prognostic biomarker in prostate cancer. Frontiers in oncology, 10, 846.

Ma, X., Feng, J., Lu, M., Tang, W., Han, J., Luo, X., ... & Yang, L. (2020). microRNA‐501‐5p promotes cell proliferation and migration in gastric cancer by downregulating LPAR1. Journal of Cellular Biochemistry, 121(2), 1911-1922.

Rivera, R. R., Lin, M. E., Bornhop, E. C., & Chun, J. (2020). Conditional Lpar1 gene targeting identifies cell types mediating neuropathic pain. The FASEB Journal, 34(7), 8833.

Cui, R., Cao, G., Bai, H., & Zhang, Z. (2019). LPAR1 regulates the development of intratumoral heterogeneity in ovarian serous cystadenocarcinoma by activating the PI3K/AKT signaling pathway. Cancer cell international, 19(1), 1-13.

Wu, X. P., Yang, Y. P., She, R. X., Xing, Z. M., Chen, H. W., & Zhang, Y. W. (2019). microRNA-329 reduces bone cancer pain through the LPAR1-dependent LPAR1/ERK signal transduction pathway in mice. Therapeutic advances in medical oncology, 11, 1758835919875319.

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For research use only. Not intended for any clinical use.

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