Mouse Anti-NCAPD2 Recombinant Antibody (4C12) (CBMAB-N1366-WJ)

This product is a Mouse antibody that recognizes NCAPD2. The antibody 4C12 can be used for immunoassay techniques such as: ELISA, IF, IHC, WB.
See all NCAPD2 antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

4C12

Antibody Isotype

IgG1, κ

Application

ELISA, IF, IHC, WB

Basic Information

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.2

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

non-SMC condensin I complex, subunit D2

Introduction

Entrez Gene ID

9918

UniProt ID

Q15021

Alternative Names

Non-SMC Condensin I Complex Subunit D2; Chromosome Condensation-Related SMC-Associated Protein 1; Chromosome-Associated Protein D2; XCAP-D2 Homolog; HCAP-D2; CNAP1; Chromosome Condensation Related SMC Associated Protein 1;

Function

Regulatory subunit of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases. May target the condensin complex to DNA via its C-terminal domain (PubMed:11136719).

May promote the resolution of double-strand DNA catenanes (intertwines) between sister chromatids. Condensin-mediated compaction likely increases tension in catenated sister chromatids, providing directionality for type II topoisomerase-mediated strand exchanges toward chromatid decatenation. Required for decatenation of non-centromeric ultrafine DNA bridges during anaphase. Early in neurogenesis, may play an essential role to ensure accurate mitotic chromosome condensation in neuron stem cells, ultimately affecting neuron pool and cortex size (PubMed:27737959).

Biological Process

Cell division Source: UniProtKB-KW
Meiotic chromosome condensation Source: GO_Central
Mitotic chromosome condensation Source: UniProtKB
Positive regulation of chromosome condensation Source: ComplexPortal

Involvement in disease

Microcephaly 21, primary, autosomal recessive (MCPH21):
A form of microcephaly, a disease defined as a head circumference more than 3 standard deviations below the age, sex and ethnically matched mean. Brain weight is markedly reduced and the cerebral cortex is disproportionately small. MCPH21 features include mild intellectual disability, intrauterine growth retardation, short stature, and microcephaly.

PTM

Phosphorylated by CDK1. Its phosphorylation, as well as that of NCAPH and NCAPG subunits, activates the condensin complex and is required for chromosome condensation (By similarity).

References

Li, Z., Zheng, Y., Wu, Z., Zhuo, T., Zhu, Y., Dai, L., ... & Chen, M. (2023). NCAPD2 is a novel marker for the poor prognosis of lung adenocarcinoma and is associated with immune infiltration and tumor mutational burden. Medicine, 102(3).

Dong, X., Liu, T., Li, Z., & Zhai, Y. (2023). Non-SMC condensin I complex subunit D2 (NCAPD2) reveals its prognostic and immunologic features in human cancers. Aging (Albany NY), 15(14), 7237.

He, J., Gao, R., Yang, J., Li, F., Fu, Y., Cui, J., ... & Wei, W. (2023). NCAPD2 promotes breast cancer progression through E2F1 transcriptional regulation of CDK1. Cancer Science, 114(3), 896-907.

Ma, W., Tian, Y., Shen, W., Song, Z., Yang, B., & Ye, D. (2023). The Prognostic and Immunotherapeutic Significanceof NCAPD2 in Pan-Cancer, and Its Relationship With the Proliferation and Metastasis of Hepatocellular Carcinoma.

Jing, Z., He, X., Jia, Z., Sa, Y., Yang, B., & Liu, P. (2021). NCAPD2 inhibits autophagy by regulating Ca2+/CAMKK2/AMPK/mTORC1 pathway and PARP-1/SIRT1 axis to promote colorectal cancer. Cancer Letters, 520, 26-37.

Lin, Y., Zeng, C., Lu, Z., Lin, R., & Liu, L. (2019). A novel homozygous splice‐site variant of NCAPD2 gene identified in two siblings with primary microcephaly: The second case report. Clinical Genetics, 96(1), 98-101.

Q & As

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Review & reward

Have you used Mouse Anti-NCAPD2 Recombinant Antibody (4C12)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Related Products

Mouse Anti-NCAPD2 Recombinant Antibody (CBFYH-2667)

Mouse Anti-NCAPD2 Recombinant Antibody (4C9)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Documents

Datasheet
SDS
Request for COA

Request bulk or custom quote

Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email: