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Mouse Anti-PARP2 Recombinant Antibody (4G8) (CBMAB-P0818-YC)

Provided herein is a Mouse monoclonal antibody against Human Poly(ADP-Ribose) Polymerase 2. The antibody can be used for immunoassay techniques, such as ELISA, WB.
See all PARP2 antibodies

Summary

Host Animal
Mouse
Specificity
Human, Mouse
Clone
4G8
Antibody Isotype
IgG1
Application
ELISA, WB

Basic Information

Immunogen
Recombinant mouse PARP-2 (poly(ADP-ribose) polymerase-2)
Specificity
Human, Mouse
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Poly(ADP-Ribose) Polymerase 2
Introduction
PARP2 is poly(ADP-ribosyl)transferase-like 2 protein, which contains a catalytic domain and is capable of catalyzing a poly(ADP-ribosyl)ation reaction. This protein has a catalytic domain which is homologous to that of poly (ADP-ribosyl) transferase, but lacks an N-terminal DNA binding domain which activates the C-terminal catalytic domain of poly (ADP-ribosyl) transferase. The basic residues within the N-terminal region of this protein may bear potential DNA-binding properties, and may be involved in the nuclear and/or nucleolar targeting of the protein. Two alternatively spliced transcript variants encoding distinct isoforms have been found.
Entrez Gene ID
Human10038
Mouse11546
UniProt ID
HumanQ9UGN5
MouseO88554
Alternative Names
Poly(ADP-Ribose) Polymerase 2; ADP-Ribosyltransferase (NAD+; Poly(ADP-Ribose) Polymerase)-Like 2; ADP-Ribosyltransferase Diphtheria Toxin-Like 2; Poly (ADP-Ribose) Polymerase Family, Member 2; NAD(+) ADP-Ribosyltransferase 2; Poly[ADP-Ribose] Synthase 2; EC 2.4.2.30; PADPRT-2; ADPRTL2; ADPRT-2;
Function
Poly-ADP-ribosyltransferase that mediates poly-ADP-ribosylation of proteins and plays a key role in DNA repair (PubMed:10364231, PubMed:25043379, PubMed:27471034, PubMed:32028527, PubMed:32939087).
Mediates glutamate, aspartate or serine ADP-ribosylation of proteins: the ADP-D-ribosyl group of NAD+ is transferred to the acceptor carboxyl group of target residues and further ADP-ribosyl groups are transferred to the 2'-position of the terminal adenosine moiety, building up a polymer with an average chain length of 20-30 units (PubMed:25043379, PubMed:30321391).
Serine ADP-ribosylation of proteins constitutes the primary form of ADP-ribosylation of proteins in response to DNA damage (PubMed:32939087).
Mediates glutamate and aspartate ADP-ribosylation of target proteins in absence of HPF1 (PubMed:25043379).
Following interaction with HPF1, catalyzes serine ADP-ribosylation of target proteins; HPF1 conferring serine specificity by completing the PARP2 active site (PubMed:28190768, PubMed:32028527).
PARP2 initiates the repair of double-strand DNA breaks: recognizes and binds DNA breaks within chromatin and recruits HPF1, licensing serine ADP-ribosylation of target proteins, such as histones, thereby promoting decompaction of chromatin and the recruitment of repair factors leading to the reparation of DNA strand breaks (PubMed:10364231, PubMed:32939087).
In addition to proteins, also able to ADP-ribosylate DNA: preferentially acts on 5'-terminal phosphates at DNA strand breaks termini in nicked duplex (PubMed:27471034).
Biological Process
Base-excision repairIEA:Ensembl
DNA ADP-ribosylationManual Assertion Based On ExperimentIDA:UniProtKB
DNA repairManual Assertion Based On ExperimentTAS:ProtInc
Double-strand break repairManual Assertion Based On ExperimentIDA:UniProtKB
Extrinsic apoptotic signaling pathwayIEA:Ensembl
Histone modificationManual Assertion Based On ExperimentIDA:UniProtKB
Negative regulation of neuron deathIEA:Ensembl
Peptidyl-serine ADP-ribosylationManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of cell growth involved in cardiac muscle cell developmentIEA:Ensembl
Protein ADP-ribosylationManual Assertion Based On ExperimentTAS:ProtInc
Protein auto-ADP-ribosylationManual Assertion Based On ExperimentIDA:UniProtKB
Protein poly-ADP-ribosylationManual Assertion Based On ExperimentIDA:UniProtKB
Cellular Location
Nucleus
Chromosome
Recruited to DNA damage sites.
PTM
Auto poly-ADP-ribosylated on serine residues, leading to dissociation of the PARP2-HPF1 complex from chromatin (PubMed:32939087).
Poly-ADP-ribosylated by PARP1 (By similarity).
Acetylation reduces DNA binding and enzymatic activity.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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