Mouse Anti-PCGF5 Recombinant Antibody (3C10) (CBMAB-P1044-YC)

Name: Mouse Anti-PCGF5 Recombinant Antibody (3C10)
SKU: CBMAB-P1044-YC
Rating: 5 (1 reviews)

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Datasheet

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Basic Information

Host Animal

Mouse

Clone

3C10

Application

ELISA, IF, WB

Immunogen

PCGF5 (NP_115749.2, 51-148 aa) partial recombinant protein with GST tag. Immunogen sequence: NDCPRCGNQV HETNPLEMLR LDNTLEEIIF KLVPGLREQE LERESEFWKK NKPQENGQDD TSKADKPKVD EEGDENEDDK DYHRSDPQIA ICLDCLRN

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

aa 51-148

More Infomation

Target

Full Name

polycomb group ring finger 5

Introduction

PCGF5 has ubiquitous expression in thyroid (RPKM 14.7), adrenal (RPKM 12.4) and 25 other tissues.

Entrez Gene ID

84333

UniProt ID

Q86SE9

Alternative Names

Polycomb Group Ring Finger 5; Ring Finger Protein (C3HC4 Type) 159; RING Finger Protein 159; RNF159; Polycomb Group RING Finger Protein 5;

Function

Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility (PubMed:26151332).
Within the PRC1-like complex, regulates RNF2 ubiquitin ligase activity (PubMed:26151332).
Plays a redundant role with PCGF3 as part of a PRC1-like complex that mediates monoubiquitination of histone H2A 'Lys-119' on the X chromosome and is required for normal silencing of one copy of the X chromosome in XX females (By similarity).

Biological Process

Histone H2A-K119 monoubiquitinationISS:UniProtKB
Inactivation of X chromosome by genomic imprintingISS:UniProtKB
Positive regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIDA:MGI
Regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIBA:GO_Central

Cellular Location

Nucleus
Nucleus, nucleoplasm
Recruited by the non-coding RNA Xist to specific nuclear foci that probably correspond to the inactivated X chromosome.

Yang, X., Zhou, W., Zhou, J., Li, A., Zhang, C., Fang, Z., ... & Zhang, M. (2024). Pcgf5: An important regulatory factor in early embryonic neural induction. Heliyon, 10(6).

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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