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Mouse Anti-PCNT Recombinant Antibody (30/Pericentrin) (CBMAB-P1082-YC)


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Summary

Host Animal
Mouse
Specificity
Mouse
Clone
30/Pericentrin
Antibody Isotype
IgG1
Application
WB, IF

Basic Information

Immunogen
Mouse pericentrin, AA 1692-1814
Specificity
Mouse
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Pericentrin
Introduction
PCNT binds to calmodulin and is expressed in the centrosome. It is an integral component of the pericentriolar material (PCM). The protein contains a series of coiled-coil domains and a highly conserved PCM targeting motif called the PACT domain near its C-terminus. The protein interacts with the microtubule nucleation component gamma-tubulin and is likely important to normal functioning of the centrosomes, cytoskeleton, and cell-cycle progression. Mutations in this gene cause Seckel syndrome-4 and microcephalic osteodysplastic primordial dwarfism type II.
Entrez Gene ID
5116
UniProt ID
P48725
Alternative Names
AW476095; C86676; KEN; Pcnt2; kendrin; m239Asp; m275Asp
Function
Integral component of the filamentous matrix of the centrosome involved in the initial establishment of organized microtubule arrays in both mitosis and meiosis. Plays a role, together with DISC1, in the microtubule network formation. Is an integral component of the pericentriolar material (PCM). May play an important role in preventing premature centrosome splitting during interphase by inhibiting NEK2 kinase activity at the centrosome.
Biological Process
Cilium assemblyManual Assertion Based On ExperimentIDA:MGI
Microtubule cytoskeleton organizationManual Assertion Based On ExperimentIMP:UniProtKB
Mitotic spindle organizationManual Assertion Based On ExperimentIMP:GO_Central
Positive regulation of intracellular protein transportManual Assertion Based On ExperimentIMP:UniProtKB
Signal transductionIEA:InterPro
Cellular Location
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
Centrosomal at all stages of the cell cycle. Remains associated with centrosomes following microtubule depolymerization. Colocalized with DISC1 at the centrosome.
Involvement in disease
Microcephalic osteodysplastic primordial dwarfism 2 (MOPD2):
Adults with this rare inherited condition have an average height of 100 centimeters and a brain size comparable to that of a 3-month-old baby, but are of near-normal intelligence.
PTM
Cleaved during mitotis which leads to removal of CDK5RAP2 from the centrosome and promotes centriole disengagement and subsequent centriole separation (PubMed:22722493, PubMed:25503564).
The C-terminal fragment is rapidly degraded following cleavage (PubMed:22722493).
More Infomation

Wu, J., Larreategui-Aparicio, A., Lambers, M. L., Bodor, D. L., Klaasen, S. J., Tollenaar, E., ... & Kops, G. J. (2023). Microtubule nucleation from the fibrous corona by LIC1-pericentrin promotes chromosome congression. Current Biology, 33(5), 912-925.

Majumder, S., Chattopadhyay, A., Wright, J. M., Guan, P., Buja, L. M., Kwartler, C. S., & Milewicz, D. M. (2023). Pericentrin deficiency in smooth muscle cells augments atherosclerosis through HSF1-driven cholesterol biosynthesis and PERK activation. JCI insight, 8(21).

Llanos, M., Wang, X., Herlitz, L. C., Shad, F., Hijazi, F. A., & Roberts, M. B. (2022). Novel Renal Phenotype of a Rare Genetic Syndrome: Pericentrin (PCNT) Gene Related Microcephalic Osteodysplastic Primordial Dwarfism Type II: FR-PO306. Journal of the American Society of Nephrology, 33(11S), 408.

Steinfeldt, J., Becker, R., Vergarajauregui, S., & Engel, F. B. (2021). Alternative splicing of pericentrin contributes to cell cycle control in cardiomyocytes. Journal of Cardiovascular Development and Disease, 8(8), 87.

Watanabe, S., Meitinger, F., Shiau, A. K., Oegema, K., & Desai, A. (2020). Centriole-independent mitotic spindle assembly relies on the PCNT–CDK5RAP2 pericentriolar matrix. Journal of Cell Biology, 219(12), e202006010.

Watanabe, K., Takao, D., Ito, K. K., Takahashi, M., & Kitagawa, D. (2019). The Cep57-pericentrin module organizes PCM expansion and centriole engagement. Nature communications, 10(1), 931.

Wang, L., Gong, Y., Li, C., Zu, Y., Cui, S., Wan, L., & Chen, X. (2019). Pericentrin expression in pancreatic β cells is associated impaired glucose tolerance. American Journal of Translational Research, 11(4), 2257.

Kim, J., Kim, J., & Rhee, K. (2019). PCNT is critical for the association and conversion of centrioles to centrosomes during mitosis. Journal of cell science, 132(6), jcs225789.

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For research use only. Not intended for any clinical use.

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