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Mouse Anti-RPS27A Recombinant Antibody (Ubi-1) (CBMAB-R3482-CN)

This product is a Mouse antibody that recognizes RPS27A. The antibody Ubi-1 can be used for immunoassay techniques such as: WB, IF, IHC, ELISA.
See all RPS27A antibodies

Summary

Host Animal
Mouse
Specificity
Cattle, C. elegans, Chicken, Human, Mouse, Plant
Clone
Ubi-1
Antibody Isotype
IgG1, κ
Application
WB, IF, IHC, ELISA

Basic Information

Immunogen
Purified ubiquitin conjugated to keyhole limpet hemocyanin with glutaraldehyde
Specificity
Cattle, C. elegans, Chicken, Human, Mouse, Plant
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Preservative
10mM sodium azide

Target

Full Name
ribosomal protein S27a
Introduction
Ubiquitin, a highly conserved protein that has a major role in targeting cellular proteins for degradation by the 26S proteosome, is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin fused to an unrelated protein. This gene encodes a fusion protein consisting of ubiquitin at the N terminus and ribosomal protein S27a at the C terminus. When expressed in yeast, the protein is post-translationally processed, generating free ubiquitin monomer and ribosomal protein S27a. Ribosomal protein S27a is a component of the 40S subunit of the ribosome and belongs to the S27AE family of ribosomal proteins. It contains C4-type zinc finger domains and is located in the cytoplasm. Pseudogenes derived from this gene are present in the genome. As with ribosomal protein S27a, ribosomal protein L40 is also synthesized as a fusion protein with ubiquitin; similarly, ribosomal protein S30 is synthesized as a fusion protein with the ubiquitin-like protein fubi. Multiple alternatively spliced transcript variants that encode the same proteins have been identified. [provided by RefSeq, Sep 2008]
Entrez Gene ID
Human6233
Mouse78294
Chicken395796
Cattle286839
UniProt ID
HumanP62979
MouseP62983
ChickenP79781
CattleP62992
Alternative Names
Ribosomal Protein S27a; Ubiquitin Carboxyl Extension Protein 80; UBCEP1; UBA80; Ubiquitin And Ribosomal Protein S27a; Ubiquitin-40S Ribosomal Protein S27a; Epididymis Luminal Protein 112; 40S Ribosomal Protein S27a;
Function
Ubiquitin
Exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in proteotoxic stress response and cell cycle; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling.
40S ribosomal protein S27a
Component of the 40S subunit of the ribosome.
Biological Process
Biological Process cytoplasmic translation1 PublicationIC:FlyBase
Biological Process modification-dependent protein catabolic processManual Assertion Based On ExperimentIBA:GO_Central
Biological Process protein ubiquitinationManual Assertion Based On ExperimentIBA:GO_Central
Biological Process translation2 PublicationsIC:UniProtKB
Cellular Location
Ubiquitin
Cytoplasm
Nucleus
PTM
Ubiquitin
Phosphorylated at Ser-65 by PINK1 during mitophagy (PubMed:24660806, PubMed:24751536, PubMed:24784582, PubMed:25527291).
Phosphorylated ubiquitin specifically binds and activates parkin (PRKN), triggering mitophagy (PubMed:24660806, PubMed:24751536, PubMed:24784582, PubMed:25527291).
Phosphorylation does not affect E1-mediated E2 charging of ubiquitin but affects discharging of E2 enzymes to form polyubiquitin chains. It also affects deubiquitination by deubiquitinase enzymes such as USP30 (PubMed:25527291).
Ubiquitin
Mono-ADP-ribosylated at the C-terminus by PARP9, a component of the PPAR9-DTX3L complex. ADP-ribosylation requires processing by E1 and E2 enzymes and prevents ubiquitin conjugation to substrates such as histones.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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