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Mouse Anti-USP10 Recombinant Antibody (CBXU-066) (CBMAB-U0176-CQ)


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Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
CBXU-066
Antibody Isotype
IgG
Application
WB, IF, IP

Basic Information

Specificity
Human, Mouse, Rat
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Concentration
1 mg/mL
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Ubiquitin Specific Peptidase 10
Introduction
This gene encodes a member of the ubiquitin-specific protease family of cysteine proteases. The enzyme specifically cleaves ubiquitin from ubiquitin-conjugated protein substrates.
Entrez Gene ID
Human9100
Mouse22224
Rat307905
UniProt ID
HumanQ14694
MouseP52479
RatQ3KR59
Alternative Names
UBPO
Function
Hydrolase that can remove conjugated ubiquitin from target proteins such as p53/TP53, BECN1, SNX3 and CFTR. Acts as an essential regulator of p53/TP53 stability: in unstressed cells, specifically deubiquitinates p53/TP53 in the cytoplasm, leading to counteract MDM2 action and stabilize p53/TP53. Following DNA damage, translocates to the nucleus and deubiquitinates p53/TP53, leading to regulate the p53/TP53-dependent DNA damage response. Component of a regulatory loop that controls autophagy and p53/TP53 levels: mediates deubiquitination of BECN1, a key regulator of autophagy, leading to stabilize the PIK3C3/VPS34-containing complexes. In turn, PIK3C3/VPS34-containing complexes regulate USP10 stability, suggesting the existence of a regulatory system by which PIK3C3/VPS34-containing complexes regulate p53/TP53 protein levels via USP10 and USP13. Does not deubiquitinate MDM2. Deubiquitinates CFTR in early endosomes, enhancing its endocytic recycling. Involved in a TANK-dependent negative feedback response to attenuate NF-kappaB activation via deubiquitinating IKBKG or TRAF6 in response to interleukin-1-beta (IL1B) stimulation or upon DNA damage (PubMed:25861989).
Deubiquitinates TBX21 leading to its stabilization (PubMed:24845384).
Biological Process
Biological Process autophagy Source:UniProtKB-KW
Biological Process cellular response to DNA damage stimulus Source:UniProtKB1 Publication
Biological Process cellular response to interleukin-1 Source:UniProtKB1 Publication
Biological Process DNA damage response, signal transduction by p53 class mediator Source:UniProtKB1 Publication
Biological Process negative regulation of I-kappaB kinase/NF-kappaB signaling Source:UniProtKB1 Publication
Biological Process protein deubiquitination Source:UniProtKB1 Publication
Biological Process regulation of autophagy Source:UniProtKB1 Publication
Biological Process translesion synthesis Source:Reactome
Biological Process ubiquitin-dependent protein catabolic process Source:InterPro
Cellular Location
Cytoplasm
Nucleus
Early endosome
Cytoplasmic in normal conditions (PubMed:20096447).
After DNA damage, translocates to the nucleus following phosphorylation by ATM (PubMed:20096447).
PTM
Phosphorylated by ATM following DNA damage, leading to stablization and translocation it to the nucleus.
Ubiquitinated. Deubiquitinated by USP13.
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For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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