Search :
Sign in or Register  
Welcome Sign in or Don't have an account?Register

Mouse Anti-USP10 Recombinant Antibody (1H9) (CBMAB-U0041-CQ)


See all USP10 antibodies
Online Inquiry

Summary

Host Animal
Mouse
Specificity
Human, Monkey
Clone
1H9
Antibody Isotype
IgG2b
Application
FC, IHC, WB

Basic Information

Immunogen
Full length recombinant protein of human USP10 produced in HEK293T cells
Specificity
Human, Monkey
Antibody Isotype
IgG2b
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.3, 1% BSA, 50% glycerol
Preservative
0.02% sodium azide
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Ubiquitin Specific Peptidase 10
Introduction
This gene encodes a member of the ubiquitin-specific protease family of cysteine proteases. The enzyme specifically cleaves ubiquitin from ubiquitin-conjugated protein substrates.
Entrez Gene ID
Human9100
Monkey718471
UniProt ID
HumanQ14694
MonkeyF6WWS6
Alternative Names
UBPO
Function
Hydrolase that can remove conjugated ubiquitin from target proteins such as p53/TP53, BECN1, SNX3 and CFTR. Acts as an essential regulator of p53/TP53 stability: in unstressed cells, specifically deubiquitinates p53/TP53 in the cytoplasm, leading to counteract MDM2 action and stabilize p53/TP53. Following DNA damage, translocates to the nucleus and deubiquitinates p53/TP53, leading to regulate the p53/TP53-dependent DNA damage response. Component of a regulatory loop that controls autophagy and p53/TP53 levels: mediates deubiquitination of BECN1, a key regulator of autophagy, leading to stabilize the PIK3C3/VPS34-containing complexes. In turn, PIK3C3/VPS34-containing complexes regulate USP10 stability, suggesting the existence of a regulatory system by which PIK3C3/VPS34-containing complexes regulate p53/TP53 protein levels via USP10 and USP13. Does not deubiquitinate MDM2. Deubiquitinates CFTR in early endosomes, enhancing its endocytic recycling. Involved in a TANK-dependent negative feedback response to attenuate NF-kappaB activation via deubiquitinating IKBKG or TRAF6 in response to interleukin-1-beta (IL1B) stimulation or upon DNA damage (PubMed:25861989).
Deubiquitinates TBX21 leading to its stabilization (PubMed:24845384).
Biological Process
Biological Process autophagy Source:UniProtKB-KW
Biological Process cellular response to DNA damage stimulus Source:UniProtKB1 Publication
Biological Process cellular response to interleukin-1 Source:UniProtKB1 Publication
Biological Process DNA damage response, signal transduction by p53 class mediator Source:UniProtKB1 Publication
Biological Process negative regulation of I-kappaB kinase/NF-kappaB signaling Source:UniProtKB1 Publication
Biological Process protein deubiquitination Source:UniProtKB1 Publication
Biological Process regulation of autophagy Source:UniProtKB1 Publication
Biological Process translesion synthesis Source:Reactome
Biological Process ubiquitin-dependent protein catabolic process Source:InterPro
Cellular Location
Cytoplasm
Nucleus
Early endosome
Cytoplasmic in normal conditions (PubMed:20096447).
After DNA damage, translocates to the nucleus following phosphorylation by ATM (PubMed:20096447).
PTM
Phosphorylated by ATM following DNA damage, leading to stablization and translocation it to the nucleus.
Ubiquitinated. Deubiquitinated by USP13.
More Infomation
Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Mouse Anti-USP10 Recombinant Antibody (1H9)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Learn more

Documents

Request bulk or custom quote Online Inquiry
Second antibody and isotype control

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Online Inquiry