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Human Apoptosis Signaling Antibody Array (Membrane, 19 Targets) (Y0195) (AbAr-0195-YC)

Antibody array is a specific form of protein microarray. In this technology, capture antibodies spotted on membranes or glass slide bind to specific target proteins present in the sample. Captured proteins are visualized using chemiluminescent or Laser Scanner. The signal produced is proportional to the amount of analyte bound. The Human Apoptosis Signaling Antibody Array detects 19 phosphorylated or cleaved human Apoptotic Factors. It is suitable for all liquid sample types but intended for use with cell and tissue lysates.
Target Array
Apoptosis Signaling Array
Akt (P-Ser473) (PKB (P-Ser473)), CHK1 (P-Ser296), IKBa (P-Ser32), P53 (P-Ser15), ATM (P-Ser1981), CHK2 (P-Thr68), JNK (P-Thr183/Tyr185), SMAD2 (Ser245/250/255), BAD (P-Ser112), eIF-2a (P-Ser52), NFKBP65 (P-Ser536), TAK1 (P-Ser412), Caspase-3 (Cleaved Asp175), ERK1 (P-T202/Y204)/ERK2 (P-Y185/Y187), PARP1 (Cleaved Asp214/Gly215), Caspase-7 (Cleaved Asp198), HSP27 (P-Ser82), p27 (P-Thr198)
Species Reactivity
Sample Type
Serum, Plasma, Cell Culture Supernatant, Cell Lysates, Tissue Lysates
Multiplex Protein Detection
Assay Type
Solid Support
Detection Assay
Protocol Outline
Block membranes
Incubate with Sample
Incubate with Detection Antibody Cocktail
Incubate with HRP-Conjugated anti-IgG
Incubate with Detection Buffers
Image with chemiluminescent imaging system
Perform densitometry and analysis
Antibody Array Membrane
Labeling Reagent
Blocking Buffer
Wash Buffer
Detection Buffer
Detection Cocktail
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Once thawed, please keep reagents under suitable conditions respectively. Do not use past expiration date.
Apoptosis is a form of programmed cell death that occurs in multicellular organisms. Biochemical events lead to characteristic cell changes (morphology) and death. These changes include blebbing, cell shrinkage, nuclear fragmentation, chromatin condensation, chromosomal DNA fragmentation, and global mRNA decay. The average adult human loses between 50 and 70 billion cells each day due to apoptosis. For an average human child between the ages of 8 to 14 year old approximately 20 to 30 billion cells die per day.
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For Research Use Only. Not For Clinical Use.