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Human Dry Eye Disease Antibody Array (Glass Slide, 40 Targets) (Y0320) (AbAr-0320-YC)

Antibody array is a specific form of protein microarray. In this technology, capture antibodies spotted on membranes or glass slide bind to specific target proteins present in the sample. Captured proteins are visualized using chemiluminescent or Laser Scanner. The signal produced is proportional to the amount of analyte bound. The Human Dry Eye Disease Antibody Array can be used for quantitative measurement of 40 Human Dry Eye Disease associated cytokines. It is suitable for serum, plasma, cell culture media, other body fluids, cell and tissue lysates.
Target Array
Dry Eye Disease Array
EGF, EGF R, Eotaxin, G-CSF, GRO, HB-EGF, ICAM-1, IFNγ, IL-1α, IL-1β, IL-1ra, IL-2, IL-4, IL-5, IL-6, IL-6R, IL-7, IL-8, IL-10, IL-11, IL-12p40, IL-12p70, IL-13, IL-15, IL-16, IL-17, IP-10, Lipocalin-2, MCP-1, MIG, MIP-1α, MIP-1β, MIP-1δ, MMP-9, RANTES, TGFβ1, TIMP-1, TIMP-2, TNFα, VEGF
Species Reactivity
Sample Type
Serum, Plasma, Other Biological Fluids, Cell Lysates, Cell Culture Media, Tissue Lysates
Multiplex Protein Detection
Assay Type
Solid Support
Glass Slide
Detection Assay
Protocol Outline
Dry the glass slide
Incubate samples
Incubate with biotin lectin mixture
Incubate with Cy3 Equivalent Dye-Streptavidin
Disassemble the glass slide
Scan with a gene microarray laser scanner
Perform densitometry and analysis
Antibody Array Slide
Labeling Reagent
Blocking Buffer
Wash Buffer
Detection Buffer
Detection Cocktail
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Once thawed, please keep reagents under suitable conditions respectively. Do not use past expiration date.
Dry eye syndrome (DES), also known as keratoconjunctivitis sicca (KCS), is the condition of having dry eyes. Other associated symptoms include irritation, redness, discharge, and easily fatigued eyes. Dry eye occurs when either the eye does not produce enough tears or when the tears evaporate too quickly.
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For Research Use Only. Not For Clinical Use.