Sign in or Register
  |  

Human Inflammation Antibody Array (Glass Slide, 40 Targets) (Y0053) (AbAr-0053-YC)

Antibody array is a specific form of protein microarray. In this technology, capture antibodies spotted on membranes or glass slide bind to specific target proteins present in the sample. Captured proteins are visualized using chemiluminescent or Laser Scanner. The signal produced is proportional to the amount of analyte bound. The Human Inflammation Antibody Array detects 40 Human Inflammatory Factors. It is suitable for all liquid sample types.
Specifications
Target Array
Inflammation Array
Target
Eotaxin-1 (CCL11), IFN-gamma, IL-3, IL-8 (CXCL8), IL-13, MCP-1 (CCL2), MIP-1 beta (CCL4), TNF beta (TNFSF1B), Eotaxin-2 (MPIF-2/CCL24), I-309 (TCA-3/CCL1), IL-4, IL-10, IL-15, MCP-2 (CCL8), MIP-1 delta (CCL15), TNF RI (TNFRSF1A), GCSF, IL-1 alpha (IL-1 F1), IL-6, IL-11, IL-16, M-CSF, RANTES (CCL5), TNF RII (TNFRSF1B), GM-CSF, IL-1 beta (IL-1 F2), IL-6 R, IL-12 p40, IL-17A, MIG (CXCL9), TGF beta 1, PDGF-BB, ICAM-1 (CD54), IL-2, IL-7, IL-12 p70, IP-10 (CXCL10), MIP-1 alpha (CCL3), TNF alpha, TIMP-2
Species Reactivity
Human
Sample Type
Serum, Plasma, Cell Culture Supernatant, Cell Lysates, Tissue Lysates
Applications
Application
Multiplex Protein Detection
Assay Type
Semi-Quantitative
Solid Support
Glass Slide
Detection Assay
Sandwich-based
Protocol Outline
Dry the glass slide
Block array surface
Incubate with Sample
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with Streptavidin-Conjugated Fluor
Disassemble the glass slide
Scan with a gene microarray laser scanner
Perform densitometry and analysis
Components
Antibody Array Slide
Labeling Reagent
Blocking Buffer
Wash Buffer
Detection Buffer
Detection Cocktail
Others
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Once thawed, please keep reagents under suitable conditions respectively. Do not use past expiration date.
Background
Introduction
Inflammation is part of the body's immune response. Infections, wounds, and any damage to tissue would not be able to heal without an inflammatory response. Chronic inflammation can eventually cause several diseases and conditions, including some cancers and rheumatoid arthritis.
Ask a question We look forward to hearing from you.
0 reviews or Q&As
Loading...
Submit a review Submit a review and you will get a coupon.
0 reviews
Loading...
For research use only. Not intended for any clinical use.