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Human Inflammation Antibody Array (Glass Slide, 40 Targets) (Y0053) (AbAr-0053-YC)

Antibody array is a specific form of protein microarray. In this technology, capture antibodies spotted on membranes or glass slide bind to specific target proteins present in the sample. Captured proteins are visualized using chemiluminescent or Laser Scanner. The signal produced is proportional to the amount of analyte bound. The Human Inflammation Antibody Array detects 40 Human Inflammatory Factors. It is suitable for all liquid sample types.
Target Array
Inflammation Array
Eotaxin-1 (CCL11), IFN-gamma, IL-3, IL-8 (CXCL8), IL-13, MCP-1 (CCL2), MIP-1 beta (CCL4), TNF beta (TNFSF1B), Eotaxin-2 (MPIF-2/CCL24), I-309 (TCA-3/CCL1), IL-4, IL-10, IL-15, MCP-2 (CCL8), MIP-1 delta (CCL15), TNF RI (TNFRSF1A), GCSF, IL-1 alpha (IL-1 F1), IL-6, IL-11, IL-16, M-CSF, RANTES (CCL5), TNF RII (TNFRSF1B), GM-CSF, IL-1 beta (IL-1 F2), IL-6 R, IL-12 p40, IL-17A, MIG (CXCL9), TGF beta 1, PDGF-BB, ICAM-1 (CD54), IL-2, IL-7, IL-12 p70, IP-10 (CXCL10), MIP-1 alpha (CCL3), TNF alpha, TIMP-2
Species Reactivity
Sample Type
Serum, Plasma, Cell Culture Supernatant, Cell Lysates, Tissue Lysates
Multiplex Protein Detection
Assay Type
Solid Support
Glass Slide
Detection Assay
Protocol Outline
Dry the glass slide
Block array surface
Incubate with Sample
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with Streptavidin-Conjugated Fluor
Disassemble the glass slide
Scan with a gene microarray laser scanner
Perform densitometry and analysis
Antibody Array Slide
Labeling Reagent
Blocking Buffer
Wash Buffer
Detection Buffer
Detection Cocktail
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Once thawed, please keep reagents under suitable conditions respectively. Do not use past expiration date.
Inflammation is part of the body's immune response. Infections, wounds, and any damage to tissue would not be able to heal without an inflammatory response. Chronic inflammation can eventually cause several diseases and conditions, including some cancers and rheumatoid arthritis.
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For Research Use Only. Not For Clinical Use.