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Mouse Inflammation Antibody Array (Glass Slide, 40 Targets) (Y0347) (AbAr-0347-YC)

Antibody array is a specific form of protein microarray. In this technology, capture antibodies spotted on membranes or glass slide bind to specific target proteins present in the sample. Captured proteins are visualized using chemiluminescent or Laser Scanner. The signal produced is proportional to the amount of analyte bound. The Mouse Inflammation Antibody Array can be used for quantitative measurement of 40 mouse inflammatory factors. It is suitable for cell culture supernatants, plasma, serum, cell and tissue lysates, and other biological fluids.
Specifications
Target Array
Inflammation Array
Target
BLC, CD30L, Eotaxin, Eotaxin-2, Fas L, G-CSF, GM-CSF, ICAM-1, IFNγ, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-10, IL-12 p70, IL-13, IL-15, IL-17, IL-21, KC, Leptin, LIX, MCP-1, MCP-5, M-CSF, MIG, MIP-1α, MIP-1γ, PF-4, RANTES, TARC, TCA-3, TIMP-1, TNFα, TNFRI, TNFRII
Species Reactivity
Mouse
Sample Type
Cell Culture Supernatant, Serum, Plasma, Other Biological Fluids, Cell Lysates, Tissue Lysates
Applications
Application
Multiplex Protein Detection
Assay Type
Quantitative
Solid Support
Glass Slide
Detection Assay
Label-based
Protocol Outline
Dry the glass slide
Incubate samples
Incubate with biotin lectin mixture
Incubate with Cy3 Equivalent Dye-Streptavidin
Disassemble the glass slide
Scan with a gene microarray laser scanner
Perform densitometry and analysis
Components
Antibody Array Slide
Labeling Reagent
Blocking Buffer
Wash Buffer
Detection Buffer
Detection Cocktail
Others
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Once thawed, please keep reagents under suitable conditions respectively. Do not use past expiration date.
Background
Introduction
Inflammation is part of the body's immune response. Infections, wounds, and any damage to tissue would not be able to heal without an inflammatory response. Chronic inflammation can eventually cause several diseases and conditions, including some cancers and rheumatoid arthritis.
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For research use only. Not intended for any clinical use.