Porcine Inflammation Antibody Array (Membrane, 26 Targets) (Y0547) (AbAr-0547-YC)

Antibody array is a specific form of protein microarray. In this technology, capture antibodies spotted on membranes or glass slide bind to specific target proteins present in the sample. Captured proteins are visualized using chemiluminescent or Laser Scanner. The signal produced is proportional to the amount of analyte bound. The product provided herein is a Porcine Inflammation Antibody Array, which can be used for semi-quantitative detection of 26 porcine inflammation factors. It is suitable for a range of liquid forms including Cell culture supernatants, Cell lysates, Plasma, Serum and Tissue lysates.
See all Inflammation antibodies

Datasheet

Specifications

Target Array

Inflammation Array

Target

Eotaxin-1(CCL11), GM-CSF, IFN alpha, IFN-beta, IFN-gamma, IL-1 alpha(IL-1 F1), IL-1 beta(IL-1 F2), IL-1 Ra(IL-1 F3), IL-10, IL-12 p70, IL-13, IL-15, IL-17A, IL-17F, IL-18, IL-21, IL-22, IL-28B, IL-4, IL-6IL-8(CXCL8), MIF, MIG(CXCL9), MIP-1 beta(CCL4), RANTES(CCL5), TNF alpha

Species Reactivity

Porcine

Sample Type

Cell culture supernatants, Cell lysates, Plasma, Serum and Tissue lysates

Applications

Application

Multiplexed Protein Detection

Assay Type

Semi-Quantitative

Solid Support

Membrane

Detection Assay

Sandwich-based Assay

Protocol Outline

Block the array membrane surface
Incubate with Sample
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with HRP Conjugated Streptavidin
Incubate with Detection Buffers
Image with chemiluminescent imaging system
Analyze data

Components

Antibody Array Membrane
Labeling Reagent
Blocking Buffer
Washing Buffer
Detection Buffer
Detection Cocktail
Others

Storage

Store at -20°C for 6 months. Once thawed, please keep reagents under suitable conditions respectively for no more than 3 months. Do not use past expiration date.

Background

Introduction

Inflammation is part of the body's immune response. Infections, wounds, and any damage to tissue would not be able to heal without an inflammatory response. Chronic inflammation can eventually cause several diseases and conditions, including some cancers and rheumatoid arthritis.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Alternative Versions

Human Inflammation Antibody Array (Glass Slide, 40 Targets) (Y0550)

Mouse Inflammation Antibody Array (Glass Slide, 40 Targets) (Y0549)

Rat Inflammation Antibody Array (Glass Slide, 10 Targets) (Y0548)

Mouse Inflammation Antibody Array (Glass Slide, 40 Targets) (Y0347)

Human Inflammation Antibody Array (Glass Slide, 10 Targets) (Y0328)

Human Inflammation Antibody Array (Glass Slide, 10 Targets) (Y0327)

Rat Inflammation Antibody Array (Glass Slide, 10 Targets) (Y0125)

Mouse Inflammation Antibody Array (Glass Slide, 40 Targets) (Y0115)

Human Inflammation Antibody Array (Glass Slide, 40 Targets) (Y0100)

Human Inflammation Antibody Array (Glass Slide, 10 Targets) (Y0099)

Antibody Array

Human Inflammation Antibody Array (Membrane, 20 Targets) (Y0040)

Human Inflammation Antibody Array (Membrane, 20 Targets) (Y0041)

Human Inflammation Antibody Array (Membrane, 40 Targets) (Y0042)

Mouse Inflammation Antibody Array (Membrane, 40 Targets) (Y0067)

Canine Inflammation Antibody Array (Membrane, 20 Targets) (Y0219)

Bovine Inflammation Antibody Array (Membrane, 16 Targets) (Y0221)

Human Inflammation Antibody Array (Glass Slide, 40 Targets) (Y0053)

Mouse Inflammation Antibody Array (Glass Slide, 40 Targets) (Y0075)

Human Inflammation Antibody Array (Glass Slide, 10 Targets) (Y0099)

Human Inflammation Antibody Array (Glass Slide, 40 Targets) (Y0100)

Related Products

Bovine Inflammation Antibody Array (Membrane, 16 Targets) (Y0221)

Rat Inflammation Antibody Array (Glass Slide, 10 Targets) (Y0548)

Human Inflammation Antibody Array (Membrane, 20 Targets) (Y0040)

Human Inflammation Antibody Array (Glass Slide, 10 Targets) (Y0327)

Mouse Inflammation Antibody Array (Glass Slide, 40 Targets) (Y0115)

Mouse Inflammation Antibody Array (Glass Slide, 40 Targets) (Y0075)

Human Inflammation Antibody Array (Membrane, 40 Targets) (Y0042)

Mouse Inflammation Antibody Array (Glass Slide, 40 Targets) (Y0549)

Human Inflammation Antibody Array (Glass Slide, 40 Targets) (Y0550)

Human Inflammation Antibody Array (Glass Slide, 40 Targets) (Y0053)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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