Human Inflammation Antibody Array (Glass Slide, 40 Targets) (Y0550) (AbAr-0550-YC)

Antibody array is a specific form of protein microarray. In this technology, capture antibodies spotted on membranes or glass slide bind to specific target proteins present in the sample. Captured proteins are visualized using chemiluminescent or Laser Scanner. The signal produced is proportional to the amount of analyte bound. The product provided herein is a Human Inflammation Antibody Array, which can be used for semi-quantitative detection of 40 human cytokines. It is suitable for a range of liquid forms including Cell culture supernatants, Cell lysates, Plasma, Serum and Tissue lysates.
See all Inflammation antibodies

Datasheet

Specifications

Target Array

Inflammation Array

Target

BLC(CXCL13), Eotaxin-1(CCL11), Eotaxin-2(MPIF-2/CCL24), GCSF, GM-CSF, I-309(TCA-3/CCL1), ICAM-1(CD54), IFN-gamma, IL-1 alpha(IL-1 F1), IL-1 beta(IL-1 F2), IL-1 ra(IL-1 F3), IL-2, IL-4, IL-5, IL-6, IL-6 R, IL-7, IL-8(CXCL8), IL-10, IL-11, IL-12 p40, IL-12 p70, IL-13, IL-15, IL-16, IL-17A, MCP-1(CCL2), M-CSF, MIG(CXCL9), MIP-1 alpha(CCL3), MIP-1 beta(CCL4), MIP-1 delta(CCL15), PDGF-BB, RANTES(CCL5), TIMP-1, TIMP-2, TNF alpha, TNF beta(TNFSF1B), TNF RI(TNFRSF1A), TNF RII(TNFRSF1B)

Species Reactivity

Human

Sample Type

Cell culture supernatants, Cell lysates, Plasma, Serum and Tissue lysates

Applications

Application

Multiplexed Protein Detection

Assay Type

Semi-Quantitative

Solid Support

Glass Slide

Detection Assay

Sandwich-based Assay

Protocol Outline

Dry the array glass slide
Block glass slide surface
Incubate with samples
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with Fluor Conjugated Streptavidin
Scan with an antibody array laser scanner
Analyze data

Components

Antibody Array Slide
Labeling Reagent
Blocking Buffer
Washing Buffer
Detection Buffer
Detection Cocktail
Others

Storage

Store at -20°C for 6 months. Once thawed, please keep reagents under suitable conditions respectively for no more than 3 months. Do not use past expiration date.

Background

Introduction

Inflammation is part of the body's immune response. Infections, wounds, and any damage to tissue would not be able to heal without an inflammatory response. Chronic inflammation can eventually cause several diseases and conditions, including some cancers and rheumatoid arthritis.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Alternative Versions

Mouse Inflammation Antibody Array (Glass Slide, 40 Targets) (Y0549)

Rat Inflammation Antibody Array (Glass Slide, 10 Targets) (Y0548)

Porcine Inflammation Antibody Array (Membrane, 26 Targets) (Y0547)

Mouse Inflammation Antibody Array (Glass Slide, 40 Targets) (Y0347)

Human Inflammation Antibody Array (Glass Slide, 10 Targets) (Y0328)

Human Inflammation Antibody Array (Glass Slide, 10 Targets) (Y0327)

Bovine Inflammation Antibody Array (Membrane, 16 Targets) (Y0221)

Canine Inflammation Antibody Array (Membrane, 20 Targets) (Y0219)

Rat Inflammation Antibody Array (Glass Slide, 10 Targets) (Y0125)

Mouse Inflammation Antibody Array (Glass Slide, 40 Targets) (Y0115)

Antibody Array

Human Inflammation Antibody Array (Membrane, 20 Targets) (Y0040)

Human Inflammation Antibody Array (Membrane, 20 Targets) (Y0041)

Human Inflammation Antibody Array (Membrane, 40 Targets) (Y0042)

Mouse Inflammation Antibody Array (Membrane, 40 Targets) (Y0067)

Canine Inflammation Antibody Array (Membrane, 20 Targets) (Y0219)

Bovine Inflammation Antibody Array (Membrane, 16 Targets) (Y0221)

Porcine Inflammation Antibody Array (Membrane, 26 Targets) (Y0547)

Human Inflammation Antibody Array (Glass Slide, 40 Targets) (Y0053)

Mouse Inflammation Antibody Array (Glass Slide, 40 Targets) (Y0075)

Human Inflammation Antibody Array (Glass Slide, 10 Targets) (Y0099)

Related Products

Mouse Inflammation Antibody Array (Membrane, 40 Targets) (Y0067)

Human Inflammation Antibody Array (Membrane, 40 Targets) (Y0042)

Rat Inflammation Antibody Array (Glass Slide, 10 Targets) (Y0125)

Mouse Inflammation Antibody Array (Glass Slide, 40 Targets) (Y0549)

Mouse Inflammation Antibody Array (Glass Slide, 40 Targets) (Y0115)

Mouse Inflammation Antibody Array (Glass Slide, 40 Targets) (Y0075)

Human Inflammation Antibody Array (Glass Slide, 10 Targets) (Y0328)

Human Inflammation Antibody Array (Membrane, 20 Targets) (Y0040)

Human Inflammation Antibody Array (Membrane, 20 Targets) (Y0041)

Porcine Inflammation Antibody Array (Membrane, 26 Targets) (Y0547)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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