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Mouse Anti-KDM5C/Jarid1C/SMCX Recombinant Antibody (2E4-E1-G8) (CBMAB-Z0408-LY)

The product is antibody recognizes KDM5C/Jarid1C/SMCX. The antibody 2E4-E1-G8 immunoassay techniques such as: WB, ICC.
See all KDM5C/Jarid1C/SMCX antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
2E4-E1-G8
Antibody Isotype
IgG2a
Application
WB, ICC

Basic Information

Immunogen
Purified recombinant human KDM5C / Jarid1C / SMCX protein fragments expressed in E.coli
Specificity
Human
Antibody Isotype
IgG2a
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4 150 mM NaCl) with 0.02% sodium azide 50% glycerol
Concentration
1 mg/mL
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Lysine Demethylase 5C
Entrez Gene ID
8242
UniProt ID
P41229
Alternative Names
MRXJ; SMCX; MRXSJ; XE169; MRXSCJ; JARID1C; DXS1272E
Function
Histone demethylase that specifically demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code (PubMed:28262558).
Does not demethylate histone H3 'Lys-9', H3 'Lys-27', H3 'Lys-36', H3 'Lys-79' or H4 'Lys-20'. Demethylates trimethylated and dimethylated but not monomethylated H3 'Lys-4'. Participates in transcriptional repression of neuronal genes by recruiting histone deacetylases and REST at neuron-restrictive silencer elements. Represses the CLOCK-ARNTL/BMAL1 heterodimer-mediated transcriptional activation of the core clock component PER2 (By similarity).
Biological Process
Chromatin remodelingManual Assertion Based On ExperimentIBA:GO_Central
Histone H3-K4 demethylationManual Assertion Based On ExperimentIDA:MGI
Negative regulation of transcription, DNA-templatedISS:UniProtKB
Response to toxic substanceIEA:Ensembl
Rhythmic processIEA:UniProtKB-KW
Cellular Location
Nucleus
Involvement in disease
Intellectual developmental disorder, X-linked, syndromic, Claes-Jensen type (MRXSCJ):
A disorder characterized by significantly below average general intellectual functioning associated with impairments in adaptive behavior and manifested during the developmental period. MRXSCJ patients manifest mental retardation associated with variable features such as slowly progressive spastic paraplegia, seizures, facial dysmorphism.

Leonardi, E., Aspromonte, M. C., Drongitis, D., Bettella, E., Verrillo, L., Polli, R., ... & Murgia, A. (2023). Expanding the genetics and phenotypic spectrum of Lysine-specific demethylase 5C (KDM5C): a report of 13 novel variants. European Journal of Human Genetics, 31(2), 202-215.

Samanta, M. K., Gayen, S., Harris, C., Maclary, E., Murata-Nakamura, Y., Malcore, R. M., ... & Kalantry, S. (2022). Activation of Xist by an evolutionarily conserved function of KDM5C demethylase. Nature Communications, 13(1), 2602.

Lemster, A. L., Sievers, E., Pasternack, H., Lazar-Karsten, P., Klümper, N., Sailer, V., ... & Kirfel, J. (2022). Histone demethylase KDM5C drives prostate cancer progression by promoting EMT. Cancers, 14(8), 1894.

Shen, H. F., Zhang, W. J., Huang, Y., He, Y. H., Hu, G. S., Wang, L., ... & Liu, W. (2021). The dual function of KDM5C in both gene transcriptional activation and repression promotes breast cancer cell growth and tumorigenesis. Advanced Science, 8(9), 2004635.

Lin, H., Ma, N., Zhao, L., Yang, G., & Cao, B. (2020). KDM5c promotes colon cancer cell proliferation through the FBXW7-c-Jun regulatory axis. Frontiers in Oncology, 10, 535449.

Carmignac, V., Nambot, S., Lehalle, D., Callier, P., Moortgat, S., Benoit, V., ... & Thauvin‐Robinet, C. (2020). Further delineation of the female phenotype with KDM5C disease causing variants: 19 new individuals and review of the literature. Clinical Genetics, 98(1), 43-55.

Link, J. C., Wiese, C. B., Chen, X., Avetisyan, R., Ronquillo, E., Ma, F., ... & Reue, K. (2020). X chromosome dosage of histone demethylase KDM5C determines sex differences in adiposity. The Journal of clinical investigation, 130(11), 5688-5702.

Hong, Z., Wu, G., Xiang, Z. D., Xu, C. D., Huang, S. S., Li, C., ... & Wu, D. L. (2019). KDM5C is transcriptionally regulated by BRD4 and promotes castration-resistance prostate cancer cell proliferation by repressing PTEN. Biomedicine & Pharmacotherapy, 114, 108793.

Chang, S., Yim, S., & Park, H. (2019). The cancer driver genes IDH1/2, JARID1C/KDM5C, and UTX/KDM6A: crosstalk between histone demethylation and hypoxic reprogramming in cancer metabolism. Experimental & molecular medicine, 51(6), 1-17.

Vallianatos, C. N., Farrehi, C., Friez, M. J., Burmeister, M., Keegan, C. E., & Iwase, S. (2018). Altered gene-regulatory function of KDM5C by a novel mutation associated with autism and intellectual disability. Frontiers in molecular neuroscience, 11, 104.

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For research use only. Not intended for any clinical use.

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