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Mouse Anti-Pyruvate Dehydrogenase E1α/PDHA1 Recombinant Antibody (3H2-F8-B5) (CBMAB-Z0317-LY)

The product is antibody recognizes Pyruvate Dehydrogenase E1α/PDHA1. The antibody 3H2-F8-B5 immunoassay techniques such as: WB, ICC.
See all Pyruvate Dehydrogenase E1α/PDHA1 antibodies

Summary

Host Animal
Mouse
Specificity
Human, Mouse
Clone
3H2-F8-B5
Antibody Isotype
IgG1
Application
WB, ICC

Basic Information

Immunogen
Purified recombinant human Pyruvate Dehydrogenase protein fragments expressed in E.coli.
Specificity
Human, Mouse
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4 150 mM NaCl) with 0.02% sodium azide 50% glycerol
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Pyruvate Dehydrogenase E1 Alpha 1 Subunit
Entrez Gene ID
Human5160
Mouse18597
UniProt ID
HumanP08559
MouseP35486
Alternative Names
PDHA; PHE1A; PDHCE1A.
Function
The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2, and thereby links the glycolytic pathway to the tricarboxylic cycle.
Biological Process
Acetyl-CoA biosynthetic process from pyruvateManual Assertion Based On ExperimentIDA:UniProtKB
Glucose metabolic processIEA:UniProtKB-KW
Mitochondrial acetyl-CoA biosynthetic process from pyruvateIEA:Ensembl
Tricarboxylic acid cycleIEA:UniProtKB-KW
Cellular Location
Mitochondrion matrix
Involvement in disease
Pyruvate dehydrogenase E1-alpha deficiency (PDHAD):
An enzymatic defect causing primary lactic acidosis in children. It is associated with a broad clinical spectrum ranging from fatal lactic acidosis in the newborn to chronic neurologic dysfunction with structural abnormalities in the central nervous system without systemic acidosis.
PTM
Phosphorylation at Ser-232, Ser-293 and Ser-300 by PDK family kinases inactivates the enzyme; for this phosphorylation at a single site is sufficient. Dephosphorylation at all three sites, i.e. at Ser-232, Ser-293 and Ser-300, is required for reactivation.
Acetylation alters the phosphorylation pattern. Deacetylated by SIRT3 (By similarity).
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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