CD36 Antibodies

Structure of CD36

CD36 is a transmembrane glycoprotein with a molecular weight of approximately 88 kDa. Its molecular weight varies among different species, mainly due to the varying degrees of glycosylation modification.

CD36 is composed of 472 amino acids and features two transmembrane domains and a large extracellular circular structure. Its secondary structure is mainly composed of β -folding, forming hydrophobic ligand-binding pockets that can recognize various molecules, such as oxidized low-density lipoprotein (oxLDL) and long-chain fatty acids. The N-terminal and C-terminal of CD36 are both located in the cytoplasm, while the extracellular domain in the middle contains multiple glycosylation sites, which affect its stability and function. The key functional sites of this protein include the fatty acid binding domain (amino acids at positions 127-279) and the thromboreactive protein binding domain (amino acids at positions 155-183), which enable it to play a core role in metabolic regulation and inflammatory responses.

Fig. 1 CD36 exhibits multiple distinct functions in cancer cells.¹

Key structural properties of CD36:

• Double-span membrane domain
• Hydrophobic ligand-binding pocket
• Highly glycosylated modification
• Functional domain
• Disulfide bonds stabilize the structure

Functions of CD36

The main function of the CD36 gene-encoded protein is to act as a multifunctional receptor to participate in lipid metabolism and immune regulation, and at the same time play a key role in various physiological and pathological processes.

The binding of CD36 to ligands shows broad-spectrum specificity, and its affinity is significantly regulated by the membrane microenvironment (such as lipid raft distribution) and post-translational modifications (such as palmitoylation). Unlike single-function receptors, CD36 achieves diversity in signal transduction by forming multi-protein complexes (such as in coordination with TLR4/6 dimers), a characteristic that makes it a key node in metabolism-inflammation cross-regulation.

Applications of CD36 and CD36 Antibody in Literature

1. Wang, gchun, and Yongsheng Li. "CD36 tango in cancer: signaling pathways and functions." Theranostics 9.17 (2019): 4893.https://www.thno.org/v09p4893.htm

Research has found that CD36 is a transmembrane glycoprotein that participates in lipid uptake, immune recognition, and inflammation processes. It inhibits tumor angiogenesis by binding to platelet reactive protein-1, and the lipid metabolism reprogramming it mediates promotes tumor immune tolerance, making it a potential anti-cancer target.

2. Feng, William W., Hannah T. Zuppe, and Manabu Kurokawa. "The role of CD36 in cancer progression and its value as a therapeutic target." Cells 12.12 (2023): 1605.https://doi.org/10.3390/cells12121605

The article indicates that CD36 is a multifunctional scavenger receptor that promotes tumor progression in breast cancer, brain cancer and ovarian cancer by regulating fatty acid uptake, metastasis, drug resistance and immune escape. Drugs targeting CD36 (such as VT1021) have entered the late clinical stage, and their therapeutic potential is not limited to cancer but also involves metabolic diseases.

3. Maréchal, Loïze, et al. "The CD36-PPARγ pathway in metabolic disorders." International Journal of Molecular Sciences 19.5 (2018): 1529. https://doi.org/10.3390/ijms19051529

The article indicates that CD36 regulates lipid and energy metabolism by mediating the activation of PPARγ through growth hormone-releasing peptide (GHRP), improving atherosclerosis, liver cholesterol synthesis and mitochondrial generation, providing a new target for the treatment of metabolic disorders.

4. Liqun, et al. "Ligand-dependent CD36 functions in cancer progression, metastasis, immune response, and drug resistance." Biomedicine & Pharmacotherapy 168 (2023): 115834. https://doi.org/10.1016/j.biopha.2023.115834

The article indicates that CD36, as a multifunctional receptor, plays a dual role in tumor growth, metastasis, drug resistance and immunosuppression by binding to ligands such as fatty acids, oxLDL and TSP1. Its ligand-dependent function has become a new target and research challenge for cancer treatment.

5. Jiang, Muwei, et al. "CD36 as a double-edged sword in cancer." Immunology Letters 265 (2024): 7-15. https://doi.org/10.1016/j.imlet.2023.12.002

The article indicates that CD36, as a multifunctional membrane protein, plays a dual role in tumors: it can promote cancer metastasis and drug resistance through lipid transport, and also bind to TSP1 to inhibit angiogenesis. Its complex regulatory mechanisms in cancer cells and immune cells bring both challenges and opportunities to targeted therapy.

Creative Biolabs: CD36 Antibodies for Research

Creative Biolabs specializes in the production of high-quality CD36 antibodies for research and industrial applications. Our portfolio includes monoclonal antibodies tailored for ELISA, Flow Cytometry, Western blot, immunohistochemistry, and other diagnostic methodologies.

• Custom CD36 Antibody Development: Tailor-made solutions to meet specific research requirements.
• Bulk Production: Large-scale antibody manufacturing for industry partners.
• Technical Support: Expert consultation for protocol optimization and troubleshooting.
• Aliquoting Services: Conveniently sized aliquots for long-term storage and consistent experimental outcomes.

For more details on our CD36 antibodies, custom preparations, or technical support, contact us at email.