DOK2

DOK2 is constitutively tyrosine phosphorylated in hematopoietic progenitors isolated from chronic myelogenous leukemia (CML) patients in the chronic phase. It may be a critical substrate for p210(bcr/abl), a chimeric protein whose presence is associated with CML. This encoded protein binds p120 (RasGAP) from CML cells.

Full Name

Docking Protein 2

Function

DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK2 may modulate the cellular proliferation induced by IL-4, as well as IL-2 and IL-3. May be involved in modulating Bcr-Abl signaling. Attenuates EGF-stimulated MAP kinase activation (By similarity).

Biological Process

Cell surface receptor signaling pathway Source: ProtInc
Ras protein signal transduction Source: Ensembl
Signal transduction Source: ProtInc

Cellular Location

Cytosol

PTM

On immunoreceptor stimulation, phosphorylated on C-terminal tyrosine residues. Phosphorylation on Tyr-345 is required for binding to the SH2 domain of NCK. Phosphorylation on both Tyr-271 and Tyr-299 is required for interaction with RASGAP. Phosphorylated on tyrosine residues by TEK/TIE2 (By similarity).

Anti-DOK2 antibodies

Fig.3 Signaling pathways in cancers. (Creative Biolabs Authorized)

Fig.4 Protocols troubleshootings & guides. (Creative Biolabs Authorized)

Mouse Anti-dok2 Recombinant Antibody (15/p56dok2) (CBMAB-P0283-YC)

Datasheet

SDS

Target: DOK2

Host: Mouse

Antibody Isotype: IgG1

Specificity: Human, Mouse, Rat

Clone: 15/p56dok2

Application*: WB

Mouse Anti-dok2 Recombinant Antibody (P0283) (V2LY-0425-LY790)

Datasheet

SDS

Target: dok2

Host: Mouse

Antibody Isotype: IgG1, κ

Specificity: Human, Mouse, Rat

Clone: P0283

Application*: WB, IP, IF, E

Mouse Anti-DOK2 Recombinant Antibody (D1504) (V2LY-0425-LY789)

Datasheet

SDS

Target: DOK2

Host: Mouse

Antibody Isotype: IgG1, κ

Specificity: Human

Clone: D1504

Application*: WB, IP, IF, E, P

Mouse Anti-DOK2 Recombinant Antibody (1A4) (V2LY-0425-LY785)

Datasheet

SDS

Target: DOK2

Host: Mouse

Antibody Isotype: IgG1

Specificity: Human

Clone: 1A4

Application*: F, IF, WB

Mouse Anti-DOK2 Recombinant Antibody (G-3) (CBMAB-D1504-YC)

Datasheet

SDS

Target: DOK2

Host: Mouse

Antibody Isotype: IgG

Specificity: Mouse, Rat

Clone: G-3

Application*: WB, IP, IF, E

Mouse Anti-DOK2 Recombinant Antibody (3D6) (CBMAB-D1503-YC)

Datasheet

SDS

Target: DOK2

Host: Mouse

Antibody Isotype: IgG1, κ

Specificity: Human

Clone: 3D6

Application*: WB, IP, IF, E

Mouse Anti-DOK2 Recombinant Antibody (2A3) (CBMAB-D1501-YC)

Datasheet

SDS

Target: DOK2

Host: Mouse

Antibody Isotype: IgG2a

Specificity: Human

Clone: 2A3

Application*: F, IF, WB

Mouse Anti-DOK2 Recombinant Antibody (1G9) (CBMAB-D1500-YC)

Datasheet

SDS

Target: DOK2

Host: Mouse

Antibody Isotype: IgG1

Specificity: Human

Clone: 1G9

Application*: F, IF, WB

Mouse Anti-DOK2 Recombinant Antibody (1A4) (CBMAB-D1499-YC)

Datasheet

SDS

Target: DOK2

Host: Mouse

Antibody Isotype: IgG1

Specificity: Human

Clone: 1A4

Application*: WB, F, IC, IF

More Infomation

For Research Use Only. Not For Clinical Use.

(P): Predicted

* Abbreviations

IFImmunofluorescence

IHImmunohistochemistry

IPImmunoprecipitation

WBWestern Blot

EELISA

MMicroarray

CIChromatin Immunoprecipitation

FFlow Cytometry

FNFunction Assay

IDImmunodiffusion

RRadioimmunoassay

TCTissue Culture

GSGel Supershift

NNeutralization

BBlocking

AActivation

IInhibition

DDepletion

ESELISpot

DBDot Blot

MCMass Cytometry/CyTOF

CTCytotoxicity

SStimulation

AGAgonist

APApoptosis

IMImmunomicroscopy

BABioassay

CSCostimulation

EMElectron Microscopy

IEImmunoelectrophoresis

PAPeptide Array

ICImmunocytochemistry

PEPeptide ELISA

MDMeDIP

SHIn situ hybridization

IAEnzyme Immunoassay

SEsandwich ELISA

PLProximity Ligation Assay

ECELISA(Cap)

EDELISA(Det)

BIBioimaging

IOImmunoassay

LFLateral Flow Immunoassay

LALuminex Assay

CImmunohistochemistry-Frozen Sections

PImmunohistologyp-Paraffin Sections

ISIntracellular Staining for Flow Cytometry

MSElectrophoretic Mobility Shift Assay

RIRNA Binding Protein Immunoprecipitation (RIP)