GTF2B
This gene encodes the general transcription factor IIB, one of the ubiquitous factors required for transcription initiation by RNA polymerase II. The protein localizes to the nucleus where it forms a complex (the DAB complex) with transcription factors IID and IIA. Transcription factor IIB serves as a bridge between IID, the factor which initially recognizes the promoter sequence, and RNA polymerase II.
Full Name
general transcription factor IIB
Function
General transcription factor that plays a role in transcription initiation by RNA polymerase II (Pol II). Involved in the pre-initiation complex (PIC) formation and Pol II recruitment at promoter DNA (PubMed:1876184, PubMed:1946368, PubMed:1517211, PubMed:3818643, PubMed:3029109, PubMed:8413225, PubMed:8515820, PubMed:8516311, PubMed:8516312, PubMed:7601352, PubMed:9420329, PubMed:12931194, PubMed:27193682).
Together with the TATA box-bound TBP forms the core initiation complex and provides a bridge between TBP and the Pol II-TFIIF complex (PubMed:8504927, PubMed:8413225, PubMed:8515820, PubMed:8516311, PubMed:8516312).
Released from the PIC early following the onset of transcription during the initiation and elongation transition and reassociates with TBP during the next transcription cycle (PubMed:7601352).
Associates with chromatin to core promoter-specific regions (PubMed:12931194, PubMed:24441171).
Binds to two distinct DNA core promoter consensus sequence elements in a TBP-independent manner; these IIB-recognition elements (BREs) are localized immediately upstream (BREu), 5'-[GC][GC][GA]CGCC-3', and downstream (BREd), 5'-[GA]T[TGA][TG][GT][TG][TG]-3', of the TATA box element (PubMed:9420329, PubMed:16230532, PubMed:7675079, PubMed:10619841).
Modulates transcription start site selection (PubMed:10318856).
Exhibits also autoacetyltransferase activity that contributes to the activated transcription (PubMed:12931194).
Together with the TATA box-bound TBP forms the core initiation complex and provides a bridge between TBP and the Pol II-TFIIF complex (PubMed:8504927, PubMed:8413225, PubMed:8515820, PubMed:8516311, PubMed:8516312).
Released from the PIC early following the onset of transcription during the initiation and elongation transition and reassociates with TBP during the next transcription cycle (PubMed:7601352).
Associates with chromatin to core promoter-specific regions (PubMed:12931194, PubMed:24441171).
Binds to two distinct DNA core promoter consensus sequence elements in a TBP-independent manner; these IIB-recognition elements (BREs) are localized immediately upstream (BREu), 5'-[GC][GC][GA]CGCC-3', and downstream (BREd), 5'-[GA]T[TGA][TG][GT][TG][TG]-3', of the TATA box element (PubMed:9420329, PubMed:16230532, PubMed:7675079, PubMed:10619841).
Modulates transcription start site selection (PubMed:10318856).
Exhibits also autoacetyltransferase activity that contributes to the activated transcription (PubMed:12931194).
Biological Process
DNA-templated transcription, initiation Source: GO_Central
Positive regulation of core promoter binding Source: GO_Central
Protein acetylation Source: UniProtKB
RNA polymerase II core complex assembly Source: UniProtKB
RNA polymerase II preinitiation complex assembly Source: UniProtKB
Transcriptional start site selection at RNA polymerase II promoter Source: UniProtKB
Transcription by RNA polymerase II Source: ARUK-UCL
Transcription initiation from RNA polymerase II promoter Source: UniProtKB
Viral transcription Source: GO_Central
Positive regulation of core promoter binding Source: GO_Central
Protein acetylation Source: UniProtKB
RNA polymerase II core complex assembly Source: UniProtKB
RNA polymerase II preinitiation complex assembly Source: UniProtKB
Transcriptional start site selection at RNA polymerase II promoter Source: UniProtKB
Transcription by RNA polymerase II Source: ARUK-UCL
Transcription initiation from RNA polymerase II promoter Source: UniProtKB
Viral transcription Source: GO_Central
Cellular Location
Nucleus; Chromosome. Non-acetylated form colocalizes with DNA in the G0/1, S and G2 phases of the cell cycle, but not during mitosis (PubMed:24441171). Acetylated form colocalizes at transcriptionally silent mitotic chromatids during mitosis at metaphase, anaphase, and telophase phases of the cell cycle (PubMed:24441171).
PTM
Acetylated (PubMed:24441171). Autoacetylated; autoacetylation at Lys-238 stimulates transcription activation (PubMed:12931194).
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Anti-GTF2B antibodies
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CompareTarget: GTF2B
Host: Rabbit
Antibody Isotype: IgG
Specificity: Human, Mouse, Rat
Clone: CBYJT-1303
Application*: WB, IP
Target: GTF2B
Host: Mouse
Antibody Isotype: IgG2b
Specificity: Human, Mouse, Pig, Rat, Dog
Clone: 20C10
Application*: E, WB, IH, IC
Target: GTF2B
Host: Mouse
Antibody Isotype: IgG
Specificity: Human, Mouse, Rat, Cattle, Chicken, Dog, Pig
Clone: CBT1196
Application*: WB, P, IF, IC
Target: GTF2B
Host: Mouse
Antibody Isotype: IgG
Specificity: Human, Monkey
Clone: CBT1197
Application*: WB, E
Target: GTF2B
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human, Monkey
Clone: CBT4280
Application*: WB
Target: GTF2B
Host: Mouse
Antibody Isotype: IgG2b
Specificity: Human, Mouse, Rat
Clone: CBYJT-1301
Application*: WB
Target: GTF2B
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human, Mouse, Rat
Clone: CBYJT-1300
Application*: WB
Target: GTF2B
Host: Mouse
Antibody Isotype: IgG2b
Specificity: Human, Mouse, Monkey
Clone: CBYJT-1299
Application*: WB
Target: GTF2B
Host: Mouse
Antibody Isotype: IgG2b
Specificity: Human
Clone: CBYJT-1298
Application*: E, F, IP
Target: GTF2B
Host: Mouse
Antibody Isotype: IgG2b
Specificity: Human
Clone: CBYJT-1297
Application*: E, F, IP
Target: GTF2B
Host: Mouse
Antibody Isotype: IgM, κ
Specificity: Human, Mouse, Rat, Dog, Cattle, Pig
Clone: CBLG1-3038
Application*: WB, E, IP, IF
Target: GTF2B
Host: Mouse
Antibody Isotype: IgG2a, κ
Specificity: Human, Mouse, Rat
Clone: 1
Application*: WB, IP
Target: GTF2B
Host: Mouse
Antibody Isotype: IgG1, κ
Specificity: Human, Mouse, Rat, Dog, Cattle, Pig
Clone: 2A3
Application*: WB, E, IP, IF
Target: GTF2B
Host: Mouse
Antibody Isotype: IgG2b, κ
Specificity: Human, Mouse, Rat
Clone: 13k131
Application*: WB, IP
Target: GTF2B
Host: Mouse
Antibody Isotype: IgG
Specificity: Human
Clone: CBFYH-0532
Application*: WB, E
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For Research Use Only. Not For Clinical Use.
(P): Predicted
* Abbreviations
- AActivation
- AGAgonist
- APApoptosis
- BBlocking
- BABioassay
- BIBioimaging
- CImmunohistochemistry-Frozen Sections
- CIChromatin Immunoprecipitation
- CTCytotoxicity
- CSCostimulation
- DDepletion
- DBDot Blot
- EELISA
- ECELISA(Cap)
- EDELISA(Det)
- ESELISpot
- EMElectron Microscopy
- FFlow Cytometry
- FNFunction Assay
- GSGel Supershift
- IInhibition
- IAEnzyme Immunoassay
- ICImmunocytochemistry
- IDImmunodiffusion
- IEImmunoelectrophoresis
- IFImmunofluorescence
- IGImmunochromatography
- IHImmunohistochemistry
- IMImmunomicroscopy
- IOImmunoassay
- IPImmunoprecipitation
- ISIntracellular Staining for Flow Cytometry
- LALuminex Assay
- LFLateral Flow Immunoassay
- MMicroarray
- MCMass Cytometry/CyTOF
- MDMeDIP
- MSElectrophoretic Mobility Shift Assay
- NNeutralization
- PImmunohistologyp-Paraffin Sections
- PAPeptide Array
- PEPeptide ELISA
- PLProximity Ligation Assay
- RRadioimmunoassay
- SStimulation
- SESandwich ELISA
- SHIn situ hybridization
- TCTissue Culture
- WBWestern Blot
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