USO1

The protein encoded by this gene is a peripheral membrane protein which recycles between the cytosol and the Golgi apparatus during interphase. It is regulated by phosphorylation: dephosphorylated protein associates with the Golgi membrane and dissociates from the membrane upon phosphorylation. Ras-associated protein 1 recruits this protein to coat protein complex II (COPII) vesicles during budding from the endoplasmic reticulum, where it interacts with a set of COPII vesicle-associated SNAREs to form a cis-SNARE complex that promotes targeting to the Golgi apparatus. Transport from the ER to the cis/medial Golgi compartments requires the action of this gene product, GM130 and giantin in a sequential manner. [provided by RefSeq]

Full Name

USO1 homolog, vesicle docking protein (yeast)

Function

General vesicular transport factor required for intercisternal transport in the Golgi stack; it is required for transcytotic fusion and/or subsequent binding of the vesicles to the target membrane. May well act as a vesicular anchor by interacting with the target membrane and holding the vesicular and target membranes in proximity.

Biological Process

Biological Process endoplasmic reticulum to Golgi vesicle-mediated transport Source:UniProtKB
Biological Process Golgi vesicle docking Source:GO_Central1 Publication
Biological Process intracellular protein transport Source:GO_Central1 Publication
Biological Process membrane fusion Source:GO_Central1 Publication
Biological Process regulation of cellular response to insulin stimulus Source:Ensembl
Biological Process secretory granule localization Source:Ensembl
Biological Process small GTPase mediated signal transduction Source:Ensembl
Biological Process transcytosis Source:GO_Central1 Publication
Biological Process vesicle fusion with Golgi apparatus Source:InterPro

Cellular Location

Cytoplasm, cytosol
Golgi apparatus membrane
Recycles between the cytosol and the Golgi apparatus during interphase. During interphase, the phosphorylated form is found exclusively in cytosol; the unphosphorylated form is associated with Golgi apparatus membranes.

PTM

Phosphorylated in a cell cycle-specific manner; phosphorylated in interphase but not in mitotic cells. Dephosphorylated protein associates with the Golgi membrane; phosphorylation promotes dissociation.