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Mouse Anti-ADCYAP1 Recombinant Antibody (V2-356443) (CBMAB-P0026-YC)

Provided herein is a Mouse monoclonal antibody against Human Adenylate Cyclase Activating Polypeptide 1. The antibody can be used for immunoassay techniques, such as WB, ICC, IHC-P, IHC-Fr, ELISA.
See all ADCYAP1 antibodies
Published Data
Mouse Anti-ADCYAP1 Recombinant Antibody (CBYC-P006) in WB
Western blotting analysis of the expression of PACAP. Representative diagram for the sites of the prefrontal cortex transfection of PACAP RNAi or scramble control sequence (SCR). T-test, ∗P < 0.05 versus SCR group, n = 3. ...View More
Lu, L., Wu, D., Wang, K., Tang, J., & Chen, G. (2020). Beneficial effects of crocin against depression via pituitary adenylate cyclase-activating polypeptide. BioMed research international, 2020.
Mouse Anti-ADCYAP1 Recombinant Antibody (CBYC-P006) in IF
The PGD2 receptor DP1 (magenta) is expressed in vMPO neurons, co-localized with neural peptides BDNF (yellow) in putative soma and PACAP (cyan) in putative processes along the 3rd ventricle (3 replicates); scale bar, 50 µm. ...View More
Wang, T. A., Teo, C. F., Åkerblom, M., Chen, C., Tynan-La Fontaine, M., Greiner, V. J., ... & Jan, L. Y. (2019). Thermoregulation via temperature-dependent PGD2 production in mouse preoptic area. Neuron, 103(2), 309-322.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
V2-356443
Antibody Isotype
IgM
Application
WB, ICC, IP, ELISA

Basic Information

Immunogen
Amino acids 101-176 of PACAP of human origin.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgM
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
ELISA1:30-1:3,000
IF(ICC)1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Adenylate Cyclase Activating Polypeptide 1
Introduction
ADCYAP1 is a secreted proprotein that is further processed into multiple mature peptides. These peptides stimulate adenylate cyclase and increase cyclic adenosine monophosphate (cAMP) levels, resulting in the transcriptional activation of target genes. The products of this gene are key mediators of neuroendocrine stress responses.
Entrez Gene ID
116
UniProt ID
P18509
Alternative Names
PACAP
Function
Binding to its receptor activates G proteins and stimulates adenylate cyclase in pituitary cells. Promotes neuron projection development through the RAPGEF2/Rap1/B-Raf/ERK pathway. In chromaffin cells, induces long-lasting increase of intracellular calcium concentrations and neuroendocrine secretion (By similarity). Involved in the control of glucose homeostasis, induces insulin secretion by pancreatic beta cells (By similarity).
Biological Process
Activation of adenylate cyclase activity
Adenylate cyclase-activating G protein-coupled receptor signaling pathway
ATP metabolic process
Behavioral fear response
cAMP-mediated signaling
Cell-cell signaling
Cellular response to glucocorticoid stimulus
Female pregnancy
G protein-coupled receptor signaling pathway
Histamine secretion
Insulin secretion
Negative regulation of acute inflammatory response to antigenic stimulus
Negative regulation of acute inflammatory response to non-antigenic stimulus
Negative regulation of glial cell proliferation
Negative regulation of GTPase activity
Negative regulation of inflammatory response to antigenic stimulus
Negative regulation of muscle cell apoptotic process
Negative regulation of potassium ion transport
Neuron projection development
Neuropeptide signaling pathway
Ovarian follicle development
Pituitary gland development
Positive regulation of cAMP-mediated signaling
Positive regulation of cell population proliferation
Positive regulation of chemokine (C-C motif) ligand 5 production
Positive regulation of cold-induced thermogenesis
Positive regulation of cytosolic calcium ion concentration
Positive regulation of ERK1 and ERK2 cascade
Positive regulation of growth hormone secretion
Positive regulation of GTPase activity
Positive regulation of interleukin-6 production
Positive regulation of neuron projection development
Positive regulation of protein kinase activity
Positive regulation of somatostatin secretion
Positive regulation of synaptic transmission, glutamatergic
Positive regulation of transcription by RNA polymerase II
Regulation of G protein-coupled receptor signaling pathway
Regulation of oligodendrocyte progenitor proliferation
Regulation of postsynaptic membrane potential
Regulation of protein localization
Response to ethanol
Response to starvation
Sensory perception of pain
Cellular Location
Secreted

Somoskői, B., Török, D., Reglődi, D., Tamás, A., Fülöp, B. D., & Cseh, S. (2020). Possible effects of pituitary adenylate cyclase activating polypeptide (PACAP) on early embryo implantation marker HB-EGF in mouse. Reproductive biology, 20(1), 9-13.

Gargiulo, A. T., Curtis, G. R., & Barson, J. R. (2020). Pleiotropic pituitary adenylate cyclase-activating polypeptide (PACAP): Novel insights into the role of PACAP in eating and drug intake. Brain research, 1729, 146626.

Cardoso, J. C. D. R., G García, M., & Power, D. M. (2020). Corrigendum: Tracing the Origins of the Pituitary Adenylate-Cyclase Activating Polypeptide (PACAP). Frontiers in Neuroscience, 14, 801.

Johnson, G. C., Parsons, R., May, V., & Hammack, S. E. (2020). The role of pituitary adenylate cyclase-activating polypeptide (PACAP) signaling in the hippocampal dentate gyrus. Frontiers in Cellular Neuroscience, 14, 111.

Johnson, G. C., Parsons, R. L., May, V., & Hammack, S. E. (2020). Pituitary adenylate cyclase-activating polypeptide-induced PAC1 receptor internalization and recruitment of MEK/ERK signaling enhance excitability of dentate gyrus granule cells. American Journal of Physiology-Cell Physiology, 318(5), C870-C878.

Denes, V., Geck, P., Mester, A., & Gabriel, R. (2019). Pituitary adenylate cyclase-activating polypeptide: 30 years in research spotlight and 600 million years in service. Journal of clinical medicine, 8(9), 1488.

Johnson, G. C., May, V., Parsons, R. L., & Hammack, S. E. (2019). Parallel signaling pathways of pituitary adenylate cyclase activating polypeptide (PACAP) regulate several intrinsic ion channels. Annals of the New York Academy of Sciences, 1455(1), 105.

Mijiddorj, T., Kanasaki, H., Oride, A., Hara, T., Sukhbaatar, U., Tumurbaatar, T., & Kyo, S. (2017). Interaction between kisspeptin and adenylate cyclase-activating polypeptide 1 on the expression of pituitary gonadotropin subunits: a study using mouse pituitary lbetaT2 cells. Biology of reproduction, 96(5), 1043-1051.

Illés, A., Opper, B., Reglodi, D., Kerényi, M., Czetany, P., Boronkai, A., ... & Horváth, G. (2017). Effects of pituitary adenylate cyclase activating polypeptide on small intestinal INT 407 cells. Neuropeptides, 65, 106-113.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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