Mouse Anti-ADCYAP1R1 Recombinant Antibody (V2-12522) (CBMAB-1012-CN)

This product is a mouse antibody that recognizes ADCYAP1R1 of human. The antibody CBLNP-192 can be used for immunoassay techniques such as: IF, WB.
See all ADCYAP1R1 antibodies

Published Data

Mouse Anti-ADCYAP1R1 Recombinant Antibody (CBLNP-192) (CBMAB-1012-CN) in WB

Western blots and plotted graphs comparing protein levels of PACAP in the sham vs. the BCAS group (n=5). ...View More

Guo, X., Tian, Y., Yang, Y., Li, S., Guo, L., & Shi, J. (2021). Pituitary adenylate cyclase-activating polypeptide protects against cognitive impairment caused by chronic cerebral hypoperfusion. Molecular Neurobiology, 58(9), 4309-4322.

Mouse Anti-ADCYAP1R1 Recombinant Antibody (CBLNP-192) (CBMAB-1012-CN) in Immunoblot

Representative immunoblots showing expression of VPAC1, VPAC2, and PAC1 receptors in tissue lysates from duodenum of wild-type (VIP+/+), VIP-KO (VIP−/−), and VIP-KO mice that received intraperitoneal injections of VIP for 3 wk (VIP−/− after treatment). Expression levels in lysates from two different mice in each group are presented. ...View More

Alcolado, N. G., Conrad, D. J., Poroca, D., Li, M., Alshafie, W., Chappe, F. G., ... & Chappe, V. M. (2014). Cystic fibrosis transmembrane conductance regulator dysfunction in VIP knockout mice. American Journal of Physiology-Cell Physiology, 307(2), C195-C207.

Mouse Anti-ADCYAP1R1 Recombinant Antibody (CBLNP-192) (CBMAB-1012-CN) in WB

Western blots showing the presence of VPAC1 receptor in JME/CF15 cells. Cell lysates were subjected to SDS-polyacrylamide gel electrophoresis and the presence of VIP receptors revealed by Western blotting with monoclonal antibodies specific for PACAP receptor. Lane 1, lysate from BHK cells were used as negative controls; lane 2, lysates from JME/CF15 cells ...View More

Rafferty, S., Alcolado, N., Norez, C., Chappe, F., Pelzer, S., Becq, F., & Chappe, V. (2009). Rescue of functional F508del cystic fibrosis transmembrane conductance regulator by vasoactive intestinal peptide in the human nasal epithelial cell line JME/CF15. Journal of Pharmacology and Experimental Therapeutics, 331(1), 2-13.

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

V2-12522

Antibody Isotype

IgG1

Application

IP, WB, ELISA

Basic Information

Immunogen

Raised against recombinant PACAP Receptor of human origin.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate).
ELISA	1:30-1:3,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin.

Preservative

< 0.1% sodium azide

Concentration

0.1 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

ADCYAP Receptor Type I

Introduction

This gene encodes type I adenylate cyclase activating polypeptide receptor, which is a membrane-associated protein and shares significant homology with members of the glucagon/secretin receptor family. This receptor mediates diverse biological actions of adenylate cyclase activating polypeptide 1 and is positively coupled to adenylate cyclase. Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified. This is a receptor for PACAP-27 and PACAP-38. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. The protein may regulate the release of adrenocorticotropin, luteinizing hormone, growth hormone, prolactin, epinephrine, and catecholamine. The protein may play a role in spermatogenesis and sperm motility. Causes smooth muscle relaxation and secretion in the gastrointestinal tract.

Entrez Gene ID

Human	117
Rat	24167

UniProt ID

Human	P41586
Rat	P32215

Alternative Names

PAC1; PAC1R; PACAPR; PACAPRI

Function

This is a receptor for PACAP-27 and PACAP-38. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. May regulate the release of adrenocorticotropin, luteinizing hormone, growth hormone, prolactin, epinephrine, and catecholamine. May play a role in spermatogenesis and sperm motility. Causes smooth muscle relaxation and secretion in the gastrointestinal tract.

Biological Process

Adenylate cyclase-modulating G protein-coupled receptor signaling pathway
Cell differentiation
Cell surface receptor signaling pathway
G protein-coupled receptor signaling pathway
Multicellular organism development
Negative regulation of inflammatory response to antigenic stimulus
Spermatogenesis

Cellular Location

Cell membrane

Topology

Extracellular: 21-155 aa
Helical: 156-178 aa
Cytoplasmic: 179-186 aa
Helical: 187-205 aa
Extracellular: 206-227 aa
Helical: 228-253 aa
Cytoplasmic: 254-268 aa
Helical: 269-291 aa
Extracellular: 292-309 aa
Helical: 310-332 aa
Cytoplasmic: 333-350 aa
Helical: 351-371 aa
Extracellular: 372-385 aa
Helical: 386-405 aa
Cytoplasmic: 406-468 aa

References

Mamoor, S. (2021). Differential expression of ADCYAP receptor type I in cancers of the breast.

Girard, B. M., Campbell, S. E., Beca, K. I., Perkins, M., Hsiang, H., May, V., & Vizzard, M. A. (2020). Intrabladder PAC1 receptor antagonist, PACAP (6-38), reduces urinary bladder frequency and pelvic sensitivity in mice exposed to repeated variate stress (RVS). Journal of Molecular Neuroscience, 1-14.

Ross, R. A., Hoeppner, S. S., Hellberg, S. N., O’Day, E. B., Rosencrans, P. L., Ressler, K. J., ... & Simon, N. M. (2020). Circulating PACAP peptide and PAC1R genotype as possible transdiagnostic biomarkers for anxiety disorders in women: a preliminary study. Neuropsychopharmacology, 45(7), 1125-1133.

Liao, C., May, V., & Li, J. (2019). PAC1 receptors: shapeshifters in motion. Journal of Molecular Neuroscience, 68(3), 331-339.

Parsons, R. L., & May, V. (2019). PACAP-induced PAC1 receptor internalization and recruitment of endosomal signaling regulate cardiac neuron excitability. Journal of Molecular Neuroscience, 68(3), 340-347.

Spence, J. P., Reiter, J. L., Qiu, B., Gu, H., Garcia, D. K., Zhang, L., ... & Liang, T. (2018). Estrogen-dependent upregulation of adcyap1r1 expression in nucleus accumbens is associated with genetic predisposition of sex-specific QTL for alcohol consumption on rat chromosome 4. Frontiers in genetics, 9, 513.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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