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Mouse Anti-AK1 Recombinant Antibody (V2-180375) (CBMAB-A1847-YC)

Provided herein is a Mouse monoclonal antibody against Human Adenylate Kinase 1. The antibody can be used for immunoassay techniques, such as FC, WB.
See all AK1 antibodies
Published Data
Mouse Anti-AK1 Recombinant Antibody (G5) in WB
Western blot for the validation of the relative expression levels of EGFR, MAP4K4, ARAF, and AK1 proteins in the paired prostate cancer cells. ...View More
Miao, W., Yuan, J., Li, L., & Wang, Y. (2019). Parallel-reaction-monitoring-based proteome-wide profiling of differential kinase protein expression during prostate cancer metastasis in vitro. Analytical chemistry, 91(15), 9893-9900.
Mouse Anti-AK1 Recombinant Antibody (G5) in WB
Western blot analyses for validating the protein expression levels of kinases in M14 cells with or without dabrafenib/vemurafenib treatment. (a) Images from Western blot analyses of the expression levels of representative kinases in M14 cells with or without dabrafenib or vemurafenib treatment. The quantification results for the ratios of kinase proteins in dabrafenib- and vemurafenib-treated cells over control untreated cells are shown in panels b and c, respectively. The data represent the mean ± S.D. of the quantification results (n = 3). ...View More
Miao, W., & Wang, Y. (2019). Quantitative interrogation of the human kinome perturbed by two BRAF inhibitors. Journal of proteome research, 18(6), 2624-2631.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
V2-180375
Antibody Isotype
IgG2a, κ
Application
IHC-F, ICC, IF, WB

Basic Information

Immunogen
Amino acids 105-194 mapping at the C-terminus of AK1 of human origin.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:30-1:3,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
adenylate kinase 1
Introduction
AK1 is an adenylate kinase enzyme involved in energy metabolism and homeostasis of cellular adenine nucleotide ratios in different intracellular compartments. This gene is highly expressed in skeletal muscle, brain and erythrocytes. Certain mutations in t
Entrez Gene ID
203
UniProt ID
P00568
Alternative Names
Adenylate Kinase 1; Adenylate Monophosphate Kinase; ATP-AMP Transphosphorylase 1; ATP:AMP Phosphotransferase; EC 2.7.4.3; Myokinase; Testis Secretory Sperm Binding Protein Li 58j;
Function
Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Also displays broad nucleoside diphosphate kinase activity. Plays an important role in cellular energy homeostasis and in adenine nucleotide metabolism.
Biological Process
ADP biosynthetic process Source: UniProtKB-UniRule
AMP metabolic process Source: UniProtKB-UniRule
ATP metabolic process Source: UniProtKB-UniRule
Nucleobase-containing small molecule interconversion Source: Reactome
Nucleoside diphosphate phosphorylation Source: UniProtKB
Nucleoside triphosphate biosynthetic process Source: UniProtKB
Cellular Location
Cytoplasm
Involvement in disease
Hemolytic anemia due to adenylate kinase deficiency (HAAKD): A disease characterized by hemolytic anemia and undetectable erythrocyte adenylate kinase activity.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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