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Mouse Anti-AKR1A1 Recombinant Antibody (V2-180451) (CBMAB-A1959-YC)

Provided herein is a Mouse monoclonal antibody against Human Aldo-Keto Reductase Family 1 Member A1. The antibody can be used for immunoassay techniques, such as IHC, IHC-P, WB.
See all AKR1A1 antibodies

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
V2-180451
Antibody Isotype
IgG1, κ
Application
IF, IP, WB, ELISA

Basic Information

Immunogen
Amino acids 205-231 within an internal region of AKR1A1 of human origin.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
IHC-P1:50-1:500
ELISA1:30-1:3,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Aldo-Keto Reductase Family 1 Member A1
Introduction
AKR1A1 is a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member, also known as aldehyde reductase, is involved in the reduction of biogenic and xenobiotic aldehydes and is present in virtua
Entrez Gene ID
10327
UniProt ID
P14550
Alternative Names
Aldo-Keto Reductase Family 1 Member A1; Aldehyde Reductase; Dihydrodiol Dehydrogenase 3; EC 1.1.1.2; ALDR1; ALR; Aldo-Keto Reductase Family 1, Member A1 (Aldehyde Reductase); Epididymis Secretory Sperm Binding Protein Li 165mP; Epididymis Secretory Protei
Function
Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols. Displays enzymatic activity towards endogenous metabolites such as aromatic and aliphatic aldehydes, ketones, monosaccharides and bile acids, with a preference for negatively charged substrates, such as glucuronate and succinic semialdehyde (PubMed:10510318). Functions as a detoxifiying enzyme by reducing a range of toxic aldehydes. Reduces methylglyoxal and 3-deoxyglucosone, which are present at elevated levels under hyperglycemic conditions and are cytotoxic. Involved also in the detoxification of lipid-derived aldehydes like acrolein (By similarity). Plays a role in the activation of procarcinogens, such as polycyclic aromatic hydrocarbon trans-dihydrodiols, and in the metabolism of various xenobiotics and drugs, including the anthracyclines doxorubicin (DOX) and daunorubicin (DAUN) (PubMed:18276838, PubMed:11306097). Displays no reductase activity towards retinoids (By similarity).
Biological Process
Aldehyde catabolic process Source: Ensembl
Cellular aldehyde metabolic process Source: ProtInc
Cellular detoxification of aldehyde Source: UniProtKB
Daunorubicin metabolic process Source: UniProtKB
D-glucuronate catabolic process Source: Ensembl
doxorubicin metabolic process Source: UniProtKB
Glucose metabolic process Source: ProtInc
Glucuronate catabolic process to xylulose 5-phosphate Source: Reactome
Glutathione derivative biosynthetic process Source: Reactome
L-ascorbic acid biosynthetic process Source: Ensembl
Cellular Location
Cytosol; Apical cell membrane
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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