Mouse Anti-AKR1A1 Recombinant Antibody (V2-180448) (CBMAB-A1956-YC)

Name: Mouse Anti-AKR1A1 Recombinant Antibody (V2-180448)
SKU: CBMAB-A1956-YC
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-AKR1A1 Recombinant Antibody (3B08) in WB

Representative (n=3) Imperial-stained SDS-PAGE gel showing SNO-proteins enriched by SNO-RAC. Mouse kidney lysates were treated with 50 µM SNO-CoA, 100 µM NADPH, and recombinant SCoRWT or SCoRK127A (as indicated) for 2 minutes. The S-nitrosylation reaction was stopped by the addition of acetone and SNO-RAC was performed. SCoR and GAPDH were assessed by western blot analysis. Imperial-stained gels were quantified using ImageJ. Scatter plate and bars represent mean ± SD. P-value <0.05 by Student's t-test for experimental lanes 6 and 7. mSCoR = endogenous mouse SCoR; hSCoR =recombinant human SCoR. ...View More

Stomberski, C. T., Zhou, H. L., Wang, L., van den Akker, F., & Stamler, J. S. (2019). Molecular recognition of S-nitrosothiol substrate by its cognate protein denitrosylase. Journal of Biological Chemistry, 294(5), 1568-1578.

Mouse Anti-AKR1A1 Recombinant Antibody (3B08) in WB

A) Western Blot analysis of protein lysates using livers from controls and MMA patients. Proteins were separated on 10% SDS-PAGE and immunoblotted with specific antibodies B) Densitometric measurements of Western Blot analysis of protein lysates using livers from controls and MMA patients. The results are shown as mean ± SD (three replicates). ...View More

Caterino, M., Chandler, R. J., Sloan, J. L., Dorko, K., Cusmano-Ozog, K., Ingenito, L., ... & Ruoppolo, M. (2016). The proteome of methylmalonic acidemia (MMA): the elucidation of altered pathways in patient livers. Molecular BioSystems, 12(2), 566-574.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

V2-180448

Application

IF, IHC, IP, WB, ELISA

Immunogen

Recombinant AKR1A1 of human origin.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
IHC-P	1:50-1:500
ELISA	1:30-1:3,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.1 mg/ml

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Aldo-Keto Reductase Family 1 Member A1

Introduction

AKR1A1 is a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member, also known as aldehyde reductase, is involved in the reduction of biogenic and xenobiotic aldehydes and is present in virtua

Entrez Gene ID

10327

UniProt ID

P14550

Alternative Names

Aldo-Keto Reductase Family 1 Member A1; Aldehyde Reductase; Dihydrodiol Dehydrogenase 3; EC 1.1.1.2; ALDR1; ALR; Aldo-Keto Reductase Family 1, Member A1 (Aldehyde Reductase); Epididymis Secretory Sperm Binding Protein Li 165mP; Epididymis Secretory Protei

Function

Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols. Displays enzymatic activity towards endogenous metabolites such as aromatic and aliphatic aldehydes, ketones, monosaccharides and bile acids, with a preference for negatively charged substrates, such as glucuronate and succinic semialdehyde (PubMed:10510318). Functions as a detoxifiying enzyme by reducing a range of toxic aldehydes. Reduces methylglyoxal and 3-deoxyglucosone, which are present at elevated levels under hyperglycemic conditions and are cytotoxic. Involved also in the detoxification of lipid-derived aldehydes like acrolein (By similarity). Plays a role in the activation of procarcinogens, such as polycyclic aromatic hydrocarbon trans-dihydrodiols, and in the metabolism of various xenobiotics and drugs, including the anthracyclines doxorubicin (DOX) and daunorubicin (DAUN) (PubMed:18276838, PubMed:11306097). Displays no reductase activity towards retinoids (By similarity).

Biological Process

Aldehyde catabolic process Source: Ensembl
Cellular aldehyde metabolic process Source: ProtInc
Cellular detoxification of aldehyde Source: UniProtKB
Daunorubicin metabolic process Source: UniProtKB
D-glucuronate catabolic process Source: Ensembl
doxorubicin metabolic process Source: UniProtKB
Glucose metabolic process Source: ProtInc
Glucuronate catabolic process to xylulose 5-phosphate Source: Reactome
Glutathione derivative biosynthetic process Source: Reactome
L-ascorbic acid biosynthetic process Source: Ensembl

Cellular Location

Cytosol; Apical cell membrane

Stomberski, C. T., Anand, P., Venetos, N. M., Hausladen, A., Zhou, H. L., Premont, R. T., & Stamler, J. S. (2019). AKR1A1 is a novel mammalian S-nitroso-glutathione reductase. Journal of Biological Chemistry, 294(48), 18285-18293.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Antibody Pairs

AKR1A1 Matched Antibody Pair (051) (CAT#: APMAB-051LY)

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AKR1A1 Matched Antibody Pair (051) (CAT#: APMAB-051LY)

Mouse Anti-AKR1A1 Recombinant Antibody (V2-180449) (CAT#: CBMAB-A1957-YC)

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Mouse Anti-AKR1A1 Recombinant Antibody (V2-180450) (CAT#: CBMAB-A1958-YC)

Mouse Anti-AKR1A1 Recombinant Antibody (V2-180451) (CAT#: CBMAB-A1959-YC)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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