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Mouse Anti-BCAS2 Recombinant Antibody (EG364) (CBMAB-EN401-LY)

The product is antibody recognizes BCAS2 . The antibody EG364 immunoassay techniques such as: WB: 1:500~1:1000 ELISA: 1:5000.
See all BCAS2 antibodies
Published Data
Mouse Anti-BCAS2 Recombinant Antibody (EG364) (CBMAB-EN401-LY) in IP and Immunoblot
In vitro pull-down of BCAS2 using biotinylated FERM incubated with nuclear lysate extracted from control or BCAS2 knockdown MCF-7 cells. This experiment was performed three independent times. ...View More
Hou, T. Y., & Kraus, W. L. (2022). Analysis of estrogen-regulated enhancer RNAs identifies a functional motif required for enhancer assembly and gene expression. Cell reports, 39(11).

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat, Horse, Cattle, Dog
Clone
EG364
Antibody Isotype
IgG3, κ
Application
WB, IP, IF, ELISA

Basic Information

Immunogen
Amino acids 11-31 near the N-terminus of BCAS2 of human origin.
Host Species
Mouse
Specificity
Human, Mouse, Rat, Horse, Cattle, Dog
Antibody Isotype
IgG3, κ
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
BCAS2, Pre-MRNA Processing Factor
Introduction
Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. May have a scaffolding role in the spliceosome assembly as it contacts all other components of the core complex. The PRP19-CDC5L complex may also play a role in the response to DNA damage (DDR).
Entrez Gene ID
Human10286
Mouse68183
Rat295334
UniProt ID
HumanO75934
MouseQ9D287
RatB5DFM8
Alternative Names
BCAS2, Pre-MRNA Processing Factor; DNA Amplified In Mammary Carcinoma 1 Protein; Breast Carcinoma Amplified Sequence 2; Spliceosome-Associated Protein SPF 27; DAM1; Spliceosome Associated Protein, Amplified In Breast Cancer;
Function
Required for pre-mRNA splicing as component of the activated spliceosome (PubMed:28502770, PubMed:28076346, PubMed:29360106, PubMed:29301961, PubMed:30705154).
Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. May have a scaffolding role in the spliceosome assembly as it contacts all other components of the core complex. The PRP19-CDC5L complex may also play a role in the response to DNA damage (DDR).
Biological Process
mRNA splicing, via spliceosome Source: UniProtKB
RNA splicing Source: UniProtKB
RNA splicing, via transesterification reactions Source: UniProtKB
Cellular Location
Nucleus; Nucleolus
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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