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Rabbit Anti-BUB3 Recombinant Antibody (EG432) (CBMAB-EN491-LY)

The product is antibody recognizes BUB3 . The antibody EG432 immunoassay techniques such as: WB: 1:500~1:1000 ELISA: 1:1000.
See all BUB3 antibodies

Summary

Host Animal
Rabbit
Specificity
Human, Mouse, Rat
Clone
EG432
Antibody Isotype
IgG
Application
WB: 1:500~1:1000 ELISA: 1:1000

Basic Information

Immunogen
The antibody was produced against synthesized peptide derived from C-terminal of human BUB3.
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
BUB3, Mitotic Checkpoint Protein
Introduction
This gene encodes a protein involved in spindle checkpoint function. The encoded protein contains four WD repeat domains and has sequence similarity with the yeast BUB3 protein. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
Entrez Gene ID
Human9184
Mouse12237
Rat361662
UniProt ID
HumanO43684
MouseQ9WVA3
RatD4A567
Alternative Names
BUB3, Mitotic Checkpoint Protein; BUB3 (Budding Uninhibited By Benzimidazoles 3, Yeast) Homolog; Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast); BUB3 Budding Uninhibited By Benzimidazoles 3 Homolog; Budding Uninhibited By Benzimidazoles 3 Homolog; Mitotic Checkpoint Protein BUB3;
Function
Has a dual function in spindle-assembly checkpoint signaling and in promoting the establishment of correct kinetochore-microtubule (K-MT) attachments. Promotes the formation of stable end-on bipolar attachments. Necessary for kinetochore localization of BUB1. Regulates chromosome segregation during oocyte meiosis. The BUB1/BUB3 complex plays a role in the inhibition of anaphase-promoting complex or cyclosome (APC/C) when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1.
Biological Process
Anaphase-promoting complex-dependent catabolic process Source: Reactome
Attachment of spindle microtubules to kinetochore Source: UniProtKB
Cell division Source: UniProtKB-KW
Meiotic cell cycle Source: UniProtKB-KW
Mitotic sister chromatid segregation Source: Ensembl
Mitotic spindle assembly checkpoint Source: GO_Central
Mitotic spindle organization Source: Reactome
Protein localization to kinetochore Source: UniProtKB
Regulation of exit from mitosis Source: Reactome
Regulation of mitotic cell cycle phase transition Source: Reactome
Ubiquitin-dependent protein catabolic process Source: Reactome
Cellular Location
Nucleus; Kinetochore. Starts to localize at kinetochores in prometaphase I (Pro-MI) stage and maintains the localization until the metaphase I-anaphase I (MI-AI) transition.
PTM
Poly-ADP-ribosylated by PARP1.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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