Mouse Anti-Bub3 Recombinant Antibody (CBYY-0959) (CBMAB-0962-YY)

Name: Mouse Anti-Bub3 Recombinant Antibody (CBYY-0959)
SKU: CBMAB-0962-YY
Rating: 5 (1 reviews)

See all Bub3 antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBYY-0959

Application

Immunogen

Derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human BUB5 protein.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2b

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 10% Glycerol

Preservative

0.02% sodium azide

Concentration

1 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

BUB3, Mitotic Checkpoint Protein

Introduction

This gene encodes a protein involved in spindle checkpoint function. The encoded protein contains four WD repeat domains and has sequence similarity with the yeast BUB3 protein. Alternate transcriptional splice variants, encoding different isoforMouse, have been characterized.

Entrez Gene ID

Human	9184
Mouse	12237
Rabbit	100352365
Monkey	450800

UniProt ID

Human	O43684
Mouse	Q9WVA3
Rabbit	G1TDQ3
Monkey	A0A0D9QWZ1

Alternative Names

BUB3, Mitotic Checkpoint Protein; BUB3 (Budding Uninhibited By Benzimidazoles 3, Yeast) Homolog; Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast); BUB3 Budding Uninhibited By Benzimidazoles 3 Homolog; Budding Uninhibited By Benzimidazoles 3 Homolog; Mitotic Checkpoint Protein BUB3;

Function

Has a dual function in spindle-assembly checkpoint signaling and in promoting the establishment of correct kinetochore-microtubule (K-MT) attachments. Promotes the formation of stable end-on bipolar attachments. Necessary for kinetochore localization of BUB1. Regulates chromosome segregation during oocyte meiosis. The BUB1/BUB3 complex plays a role in the inhibition of anaphase-promoting complex or cyclosome (APC/C) when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1.

Biological Process

Anaphase-promoting complex-dependent catabolic process Source: Reactome
Attachment of spindle microtubules to kinetochore Source: UniProtKB
Cell division Source: UniProtKB-KW
Meiotic cell cycle Source: UniProtKB-KW
Mitotic sister chromatid segregation Source: Ensembl
Mitotic spindle assembly checkpoint Source: GO_Central
Mitotic spindle organization Source: Reactome
Protein localization to kinetochore Source: UniProtKB
Regulation of exit from mitosis Source: Reactome
Regulation of mitotic cell cycle phase transition Source: Reactome
Ubiquitin-dependent protein catabolic process Source: Reactome

Cellular Location

Nucleus; Kinetochore. Starts to localize at kinetochores in prometaphase I (Pro-MI) stage and maintains the localization until the metaphase I-anaphase I (MI-AI) transition.

PTM

Poly-ADP-ribosylated by PARP1.

Elias Godoy Carlos, J. A., & Machado-Neto, J. A. (2020). BUB3 (BUB3 mitotic checkpoint protein). Atlas of Genetics and Cytogenetics in Oncology and Haematology.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Mouse Anti-Bub3 Recombinant Antibody (CBYY-0959)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

Mouse Anti-Bub3 Recombinant Antibody (CBYY-2014) (CAT#: CBMAB-2363-YY)

Rabbit Anti-Bub3 Recombinant Antibody (CBYY-0958) (CAT#: CBMAB-0961-YY)

Mouse Anti-Bub3 Recombinant Antibody (CBYY-2006) (CAT#: CBMAB-2355-YY)

Rabbit Anti-BUB3 Recombinant Antibody (D8G6) (CAT#: CBMAB-CP0046-LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry