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Mouse Anti-CA9 Recombinant Antibody (IHC524) (CBMAB-MA026-YC)

Provided herein is a Mouse monoclonal antibody against Carbonic Anhydrase 9. The antibody can be used for immunoassay techniques, such as IHC. And it gave a positive signal in Clear Cell Renal Cell Carcinoma.
See all CA9 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
IHC524
Application
IHC (1:100 - 1:200)

Basic Information

Specificity
Human
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
Tris buffer pH 7.3 - 7.7, 1% BSA, < 0.1% Sodium Azide
Storage
Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

Target

Full Name
carbonic anhydrase 9
Introduction
Carbonic anhydrase IX is an enzyme that in humans is encoded by the CA9 gene. It is one of the 14 carbonic anhydrase isoforms found in humans and is a transmembrane dimeric metalloenzyme with an extracellular active site that facilitates acid secretion in the gastrointestinal tract.
Entrez Gene ID
768
UniProt ID
Q16790
Alternative Names
MN; CAIX
Function
Reversible hydration of carbon dioxide. Participates in pH regulation. May be involved in the control of cell proliferation and transformation. Appears to be a novel specific biomarker for a cervical neoplasia.
Biological Process
Bicarbonate transport Source: Reactome
Morphogenesis of an epithelium Source: Ensembl
One-carbon metabolic process Source: GO_Central
Regulation of transcription from RNA polymerase II promoter in response to hypoxia Source: Reactome
Response to drug Source: Ensembl
Response to testosterone Source: Ensembl
Secretion Source: Ensembl
Cellular Location
Nucleus; Nucleolus; Cell membrane; Microvillus membrane. Found on the surface microvilli and in the nucleus, particularly in nucleolus.
Topology
Extracellular: 38-414 aa
Helical: 415-435 aa
Cytoplasmic: 436-459 aa
PTM
Asn-346 bears high-mannose type glycan structures.

Giatromanolaki, A., Harris, A. L., Banham, A. H., Contrafouris, C. A., & Koukourakis, M. I. (2020). Carbonic anhydrase 9 (CA9) expression in non-small-cell lung cancer: correlation with regulatory FOXP3+ T-cell tumour stroma infiltration. British journal of cancer, 122(8), 1205-1210.

Baniak, N., Flood, T. A., Buchanan, M., Dal Cin, P., & Hirsch, M. S. (2020). Carbonic anhydrase IX (CA9) expression in multiple renal epithelial tumour subtypes. Histopathology, 77(4), 659-666.

Wang, S., Fu, Z., Wang, Y., Sun, Y., Cui, L., Wang, C., ... & Wen, N. (2020). Correlation of carbonic anhydrase 9 (CA9) with pathological T-stage and prognosis in patients with oral tongue squamous cell carcinoma. Annals of Translational Medicine, 8(22).

Li, Z., Jiang, L., Chew, S. H., Hirayama, T., Sekido, Y., & Toyokuni, S. (2019). Carbonic anhydrase 9 confers resistance to ferroptosis/apoptosis in malignant mesothelioma under hypoxia. Redox biology, 26, 101297.

Eckert, A. W., Horter, S., Bethmann, D., Kotrba, J., Kaune, T., Rot, S., ... & Kappler, M. (2019). Investigation of the prognostic role of carbonic anhydrase 9 (CAIX) of the cellular mRNA/protein level or soluble CAIX protein in patients with oral squamous cell carcinoma. International journal of molecular sciences, 20(2), 375.

McDonald, P. C., Chafe, S. C., Brown, W. S., Saberi, S., Swayampakula, M., Venkateswaran, G., ... & Dedhar, S. (2019). Regulation of pH by carbonic anhydrase 9 mediates survival of pancreatic cancer cells with activated KRAS in response to hypoxia. Gastroenterology, 157(3), 823-837.

Finkelmeier, F., Canli, Ö., Peiffer, K. H., Walter, D., Tal, A., Koch, C., ... & Waidmann, O. (2018). Circulating hypoxia marker carbonic anhydrase IX (CA9) in patients with hepatocellular carcinoma and patients with cirrhosis. PloS one, 13(7), e0200855.

Kim, J. Y., Lee, S. H., An, S., Kim, S. J., Sung, Y. N., Song, K. B., ... & Hong, S. M. (2018). Carbonic anhydrase 9 expression in well-differentiated pancreatic neuroendocrine neoplasms might be associated with aggressive behavior and poor survival. Virchows Archiv, 472(5), 739-748.

Horie, K., Kawakami, K., Fujita, Y., Sugaya, M., Kameyama, K., Mizutani, K., ... & Ito, M. (2017). Exosomes expressing carbonic anhydrase 9 promote angiogenesis. Biochemical and biophysical research communications, 492(3), 356-361.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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