Mouse Anti-CAMSAP2 Recombinant Antibody (2A10A1) (CBMAB-C4576-LY)

This product is antibody recognizes CAMSAP2. The antibody 2A10A1 immunoassay techniques such as: ELISA, WB.
See all CAMSAP2 antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human, Mouse, Rat

Clone

2A10A1

Antibody Isotype

IgG1

Application

ELISA, WB

Basic Information

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

Calmodulin Regulated Spectrin Associated Protein Family Member 2

Introduction

CAMSAP2 (Calmodulin Regulated Spectrin Associated Protein Family Member 2) is a Protein Coding gene.

Gene Ontology (GO) annotations related to this gene include microtubule binding and microtubule minus-end binding.
An important paralog of this gene is CAMSAP1.

Entrez Gene ID

Human	23271
Mouse	67886
Rat	289400

UniProt ID

Human	Q08AD1
Mouse	Q8C1B1
Rat	D4AEC2

Function

Key microtubule-organizing protein that specifically binds the minus-end of non-centrosomal microtubules and regulates their dynamics and organization (PubMed:23169647, PubMed:24486153, PubMed:24706919).
Specifically recognizes growing microtubule minus-ends and autonomously decorates and stabilizes microtubule lattice formed by microtubule minus-end polymerization (PubMed:24486153, PubMed:24706919).
Acts on free microtubule minus-ends that are not capped by microtubule-nucleating proteins or other factors and protects microtubule minus-ends from depolymerization (PubMed:24486153, PubMed:24706919).
In addition, it also reduces the velocity of microtubule polymerization (PubMed:24486153, PubMed:24706919).
Through the microtubule cytoskeleton, also regulates the organization of cellular organelles including the Golgi and the early endosomes (PubMed:27666745).
Essential for the tethering, but not for nucleation of non-centrosomal microtubules at the Golgi: together with Golgi-associated proteins AKAP9 and PDE4DIP, required to tether non-centrosomal minus-end microtubules to the Golgi, an important step for polarized cell movement (PubMed:27666745).
Also acts as a regulator of neuronal polarity and development: localizes to non-centrosomal microtubule minus-ends in neurons and stabilizes non-centrosomal microtubules, which is required for neuronal polarity, axon specification and dendritic branch formation (PubMed:24908486).
Through the microtubule cytoskeleton, regulates the autophagosome transport (PubMed:28726242).

Biological Process

Axon development Source: UniProtKB
Cytoplasmic microtubule organization Source: GO_Central
Microtubule cytoskeleton organization Source: UniProtKB
Negative regulation of microtubule depolymerization Source: GO_Central
Regulation of dendrite development Source: UniProtKB
Regulation of Golgi organization Source: UniProtKB
Regulation of microtubule polymerization Source: UniProtKB
Regulation of organelle organization Source: UniProtKB

Cellular Location

Golgi apparatus; Cytoskeleton; Cilium basal body; Cytoplasm. Associated with the minus-end of microtubules and also detected at the centrosomes (PubMed:23169647, PubMed:24486153, PubMed:24908486, PubMed:24706919, PubMed:27666745, PubMed:28814570). Decorates the minus-end of microtubules by decreasing the rate of tubulin incorporation and remaining bound (PubMed:24486153). The length of CAMSAP2-decorated stretches on the minus-end of microtubules depends on MAPRE1/EB1 and MAPRE3/EB3, which promote elongation of CAMSAP2-decorated microtubule stretches (PubMed:28814570). Recruited to the Golgi apparatus by AKAP9 and PDE4DIP isoform 13/MMG8/SMYLE (PubMed:27666745). In neurons, localizes to the minus-end of microtubules in axon and dendrites (PubMed:24908486).

References

Imasaki, T., Kikkawa, S., Niwa, S., Saijo-Hamano, Y., Shigematsu, H., Aoyama, K., ... & Nitta, R. (2021). CAMSAP2 organizes a γ-tubulin-independent microtubule nucleation centre. bioRxiv.

Li, D., Ding, X., Xie, M., Huang, Z., Han, P., Tian, D., & Xia, L. (2020). CAMSAP2-mediated noncentrosomal microtubule acetylation drives hepatocellular carcinoma metastasis. Theranostics, 10(8), 3749.

Mao, B. P., Li, L., Ge, R., Li, C., Wong, C. K., Silvestrini, B., ... & Cheng, C. Y. (2019). CAMSAP2 is a microtubule minus-end targeting protein that regulates BTB dynamics through cytoskeletal organization. Endocrinology, 160(6), 1448-1467.

Wei, J., Xu, H., & Meng, W. (2017). Noncentrosomal microtubules regulate autophagosome transport through CAMSAP2‐EB1 cross‐talk. FEBS letters, 591(16), 2379-2393.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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