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Mouse Anti-CAPN2 Recombinant Antibody (1E8) (CBMAB-1307-CN)

This product is a mouse antibody that recognizes CAPN2 of human. The antibody 1E8 can be used for immunoassay techniques such as: IF, ELISA, WB.
See all CAPN2 antibodies

Summary

Host Animal
Mouse
Specificity
Human, Cattle
Clone
1E8
Antibody Isotype
IgG1
Application
IF, ELISA, WB

Basic Information

Immunogen
Bovine cardiac Calpain 2
Specificity
Human, Cattle
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Concentration
2 mg/mL
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Calpain 2
Introduction
The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. This protein proteolytically cleaves CPEB3 following neuronal stimulation which abolishes CPEB3 translational repressor activity, leading to translation of CPEB3 target mRNAs.
Entrez Gene ID
Human824
Cattle281662
UniProt ID
HumanP17655
CattleQ27971
Alternative Names
CANP2; mCANP; CANPL2; CANPml
Function
Calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Proteolytically cleaves MYOC at 'Arg-226' (PubMed:17650508).
Proteolytically cleaves CPEB3 following neuronal stimulation which abolishes CPEB3 translational repressor activity, leading to translation of CPEB3 target mRNAs (By similarity).
Biological Process
Behavioral response to pain Source: Ensembl
Blastocyst development Source: Ensembl
Cellular response to amino acid stimulus Source: UniProtKB
Cellular response to interferon-beta Source: Ensembl
Cellular response to lipopolysaccharide Source: Ensembl
Extracellular matrix disassembly Source: Reactome
Female pregnancy Source: Ensembl
Myoblast fusion Source: Ensembl
Positive regulation of cardiac muscle cell apoptotic process Source: Ensembl
Positive regulation of myoblast fusion Source: Ensembl
Positive regulation of neuron death Source: Ensembl
Positive regulation of phosphatidylcholine biosynthetic process Source: Ensembl
Protein autoprocessing Source: Ensembl
Proteolysis Source: UniProtKB
Proteolysis involved in cellular protein catabolic process Source: BHF-UCL
Regulation of cytoskeleton organization Source: BHF-UCL
Regulation of interleukin-6 production Source: Ensembl
Response to hydrogen peroxide Source: Ensembl
Response to hypoxia Source: Ensembl
Cellular Location
Cytoplasm; Cell membrane. Translocates to the plasma membrane upon Ca2+ binding.

Wang, J., Ciampa, G., Zheng, D., Shi, Q., Chen, B., Abel, E. D., ... & Song, L. S. (2021). Calpain-2 Specifically Cleaves Junctophilin-2 at the Same Site as Calpain-1 but with Less Efficacy. Biochemical Journal.

Kwon, S. H., Wi, T., Park, Y. I., Kim, M. W., Lee, G., Higaki, T., ... & Lee, R. (2020). Noninvasive Early Detection of Calpain 2-Enriched Non-Small Cell Lung Cancer Using a Human Serum Albumin-Bounded Calpain 2 Nanosensor. Bioconjugate chemistry, 31(3), 803-812.

Shinkai-Ouchi, F., Shindo, M., Doi, N., Hata, S., & Ono, Y. (2020). Calpain-2 participates in the process of calpain-1 inactivation. Bioscience reports, 40(11), BSR20200552.

Cicognola, C., Satir, T. M., Brinkmalm, G., Matečko-Burmann, I., Agholme, L., Bergström, P., ... & Höglund, K. (2020). Tauopathy-associated tau fragment ending at amino acid 224 is generated by Calpain-2 cleavage. Journal of Alzheimer's Disease, 74(4), 1143-1156.

Siuda, D., Randriamboavonjy, V., & Fleming, I. (2019). Regulation of calpain 2 expression by miR-223 and miR-145. Biochimica et Biophysica Acta (BBA)-Gene Regulatory Mechanisms, 1862(10), 194438.

Liu, Z. F., Ji, J. J., Zheng, D., Su, L., & Peng, T. (2019). Calpain‐2 protects against heat stress‐induced cardiomyocyte apoptosis and heart dysfunction by blocking p38 mitogen‐activated protein kinase activation. Journal of cellular physiology, 234(7), 10761-10770.

Zheng, D., Su, Z., Zhang, Y., Ni, R., Fan, G. C., Robbins, J., ... & Peng, T. (2019). Calpain-2 promotes MKP-1 expression protecting cardiomyocytes in both in vitro and in vivo mouse models of doxorubicin-induced cardiotoxicity. Archives of toxicology, 93(4), 1051-1065.

Zhang, G., Fang, T., Chang, M., Li, J., Hong, Q., Bai, C., & Zhou, J. (2018). Calpain 2 knockdown promotes cell apoptosis and restores gefitinib sensitivity through epidermal growth factor receptor/protein kinase B/survivin signaling. Oncology reports, 40(4), 1937-1946.

Liu, B., Zhou, Y., Lu, D., Liu, Y., Zhang, S. Q., Xu, Y., ... & Gu, X. (2017). Comparison of the protein expression of calpain‑1, calpain‑2, calpastatin and calmodulin between gastric cancer and normal gastric mucosa. Oncology letters, 14(3), 3705-3710.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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