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Mouse Anti-CBX5 Antibody (2D8) (CBMAB-0204-YC)

Provided herein are mouse monoclonal antibodies against Human CBX5. The antibody clone 2D8 can be used for immunoassay techniques, such as WB and MA.
See all CBX5 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
2D8
Antibody Isotype
IgG1
Application
WB, MA

Basic Information

Immunogen
Recombinant protein
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Supernatant
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Chromobox 5
Introduction
CBX5 (chromobox 5) is a highly conserved nonhistone protein, which is a member of the heterochromatin protein family. CBX5 is a highly conserved, non-histone protein part of the heterochromatin family. The protein itself is more commonly called (in humans) HP1α. Heterochromatin protein-1 (HP1) has an N-terminal domain that acts on methylated lysines residues leading to epigenetic repression. The C-terminal of this protein has a chromo shadow-domain (CSD) that is responsible for homodimerizing.
Entrez Gene ID
23468
UniProt ID
P45973
Alternative Names
Chromobox Homolog 5; Chromobox Homolog 5 (HP1 Alpha Homolog, Drosophila); Heterochromatin Protein 1 Homolog Alpha; Antigen P25; HP1A; Chromobox Homolog 5 (Drosophila HP1 Alpha); Heterochromatin Protein 1-Alpha; HP1 Alpha Homolog (Drosophila); Epididymis Luminal Protein 25; HP1 Alpha Homolog; HP1Hs Alpha; HP1-ALPHA; HP1 Alpha; HEL25; HP1
Function
Component of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when 'Tyr-41' of histone H3 is phosphorylated (H3Y41ph). Can interact with lamin-B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.
Biological Process
Blood coagulation Source: Reactome
Cellular response to DNA damage stimulus Source: UniProtKB
Negative regulation of G0 to G1 transition Source: Reactome
Negative regulation of transcription, DNA-templated Source: UniProtKB
Negative regulation of transcription by RNA polymerase II Source: UniProtKB
Viral process Source: UniProtKB-KW
Cellular Location
Nucleus; Chromosome; Centromere. Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, the RAD21 subunit of the cohesin complex is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation. The phosphorylated form at Ser-1083 is preferentially associated with unsynapsed chromosomal regions (By similarity).
PTM
Phosphorylation of HP1 and LBR may be responsible for some of the alterations in chromatin organization and nuclear structure which occur at various times during the cell cycle (By similarity). Phosphorylated during interphase and possibly hyper-phosphorylated during mitosis.
Ubiquitinated.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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