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Mouse Anti-CCND3 Recombinant Antibody (CBXC-1375) (CBMAB-C2599-CQ)

This product is a mouse antibody that recognizes CCND3. The antibody CBXC-1375 can be used for immunoassay techniques such as: IP, WB.
See all CCND3 antibodies

Summary

Host Animal
Mouse
Specificity
Human, Rat
Clone
CBXC-1375
Antibody Isotype
IgG1
Application
IP, WB

Basic Information

Immunogen
Purified human recombinant full length cyclin D3 protein
Specificity
Human, Rat
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
10 mM PBS, pH 7.4, 0.2% BSA
Preservative
15 mM sodium azide
Concentration
1 mg/mL
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Cyclin D3
Introduction
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activtiy is required for cell cycle G1/S transition. This protein has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. The CDK4 activity associated with this cyclin was reported to be necessary for cell cycle progression through G2 phase into mitosis after UV radiation. Several transcript variants encoding different isoforms have been found for this gene.
Entrez Gene ID
Human896
Rat25193
UniProt ID
HumanP30281
RatP48961
Alternative Names
Cyclin D3;
Function
Regulatory component of the cyclin D3-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G1/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G1 phase. Hypophosphorylates RB1 in early G1 phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D3/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.
Biological Process
Cell division Source: UniProtKB-KW
Mitotic cell cycle phase transition Source: GO_Central
Negative regulation of transcription by RNA polymerase II Source: Ensembl
Positive regulation of cyclin-dependent protein serine/threonine kinase activity Source: BHF-UCL
Positive regulation of G1/S transition of mitotic cell cycle Source: GO_Central
Positive regulation of protein phosphorylation Source: BHF-UCL
Regulation of cell population proliferation Source: Ensembl
Regulation of cyclin-dependent protein serine/threonine kinase activity Source: GO_Central
Regulation of insulin receptor signaling pathway Source: Ensembl
Signal transduction Source: Ensembl
T cell proliferation Source: Ensembl
Cellular Location
Cytoplasm; Nucleus; Membrane. Cyclin D-CDK4 complexes accumulate at the nuclear membrane and are then translocated to the nucleus through interaction with KIP/CIP family members.
PTM
Polyubiquitinated by the SCF(FBXL2) complex, leading to proteasomal degradation.

Smith, C. C., Viny, A. D., Massi, E., Kandoth, C., Socci, N. D., Rapaport, F., ... & Shah, N. P. (2021). Recurrent Mutations in Cyclin D3 Confer Clinical Resistance to FLT3 Inhibitors in Acute Myeloid Leukemia. Clinical Cancer Research.

Pae, J., Ersching, J., Castro, T. B., Schips, M., Mesin, L., Allon, S. J., ... & Victora, G. D. (2021). Cyclin D3 drives inertial cell cycling in dark zone germinal center B cells. Journal of Experimental Medicine, 218(4).

Wu, H., Wang, C., Liu, Y., Yang, C., Liang, X., Zhang, X., & Li, X. (2020). miR‑138‑5p suppresses glioblastoma cell viability and leads to cell cycle arrest by targeting cyclin D3. Oncology Letters, 20(5), 1-1.

Ramezani-Rad, P., Chen, C., Zhu, Z., & Rickert, R. C. (2020). Cyclin D3 governs clonal expansion of dark zone germinal center B cells. Cell reports, 33(7), 108403.

Martín-Garcia, D., Navarro, A., Valdés-Mas, R., Clot, G., Gutiérrez-Abril, J., Prieto, M., ... & Beà, S. (2019). CCND2 and CCND3 hijack immunoglobulin light-chain enhancers in cyclin D1− mantle cell lymphoma. Blood, The Journal of the American Society of Hematology, 133(9), 940-951.

Kang, H., Heo, S., Shin, J. J., Ji, E., Tak, H., Ahn, S., ... & Kim, W. (2019). A miR‐194/PTBP1/CCND3 axis regulates tumor growth in human hepatocellular carcinoma. The Journal of pathology, 249(3), 395-408.

Ding, Z. Y., Li, R., Zhang, Q. J., Wang, Y., Jiang, Y., Meng, Q. Y., ... & Wu, G. H. (2019). Prognostic role of cyclin D2/D3 in multiple human malignant neoplasms: A systematic review and meta‐analysis. Cancer medicine, 8(6), 2717-2729.

Demin, D. E., Bogolyubova, A. V., Zlenko, D. V., Uvarova, A. N., Deikin, A. V., Putlyaeva, L. V., ... & Schwartz, A. M. (2018). The novel short isoform of securin stimulates the expression of cyclin D3 and angiogenesis factors VEGFA and FGF2, but does not affect the expression of MYC transcription factor. Molecular Biology, 52(3), 436-445.

Yu, X., Zhang, Y., Cavazos, D., Ma, X., Zhao, Z., Du, L., & Pertsemlidis, A. (2018). miR-195 targets cyclin D3 and survivin to modulate the tumorigenesis of non-small cell lung cancer. Cell death & disease, 9(2), 1-12.

Curiel-Olmo, S., Mondéjar, R., Almaraz, C., Mollejo, M., Cereceda, L., Marès, R., ... & Martínez, N. (2017). Splenic diffuse red pulp small B-cell lymphoma displays increased expression of cyclin D3 and recurrent CCND3 mutations. Blood, The Journal of the American Society of Hematology, 129(8), 1042-1045.

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For research use only. Not intended for any clinical use.

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