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Mouse Anti-CCNE1 Recombinant Antibody (CBXC-2950) (CBMAB-C4707-CQ)

This product is a mouse antibody that recognizes CCNE1. The antibody CBXC-2950 can be used for immunoassay techniques such as: WB, FC, ELISA.
See all CCNE1 antibodies

Summary

Host Animal
Mouse
Specificity
Human, Mouse
Clone
CBXC-2950
Antibody Isotype
IgG1
Application
WB, FC, ELISA

Basic Information

Immunogen
Purified recombinant fragment of human CCNE1 (AA: 307-410) expressed in E. coli
Specificity
Human, Mouse
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Cyclin E1
Introduction
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, have been described. Two additional splice variants were reported but detailed nucleotide sequence information is not yet available.
Entrez Gene ID
Human898
Mouse12447
UniProt ID
HumanP24864
MouseQ61457
Alternative Names
Cyclin E1; CCNE; G1/S-Specific Cyclin-E1; Cyclin Es; Cyclin Et; PCCNE1;
Function
Essential for the control of the cell cycle at the G1/S (start) transition.
Biological Process
Cell division Source: UniProtKB-KW
DNA replication initiation Source: Ensembl
G1/S transition of mitotic cell cycle Source: BHF-UCL
Homologous chromosome pairing at meiosis Source: Ensembl
Mitotic cell cycle phase transition Source: GO_Central
Negative regulation of transcription by RNA polymerase II Source: Ensembl
Positive regulation of mesenchymal stem cell proliferation Source: BHF-UCL
Protein phosphorylation Source: MGI
Regulation of cellular protein localization Source: Ensembl
Regulation of cyclin-dependent protein serine/threonine kinase activity Source: GO_Central
Regulation of transcription involved in G1/S transition of mitotic cell cycle Source: Reactome
Telomere maintenance Source: Ensembl
Wnt signaling pathway Source: Ensembl
Cellular Location
Nucleus
PTM
Phosphorylation of both Thr-395 by GSK3 and Ser-399 by CDK2 creates a high affinity degron recognized by FBXW7, and accelerates degradation via the ubiquitin proteasome pathway. Phosphorylation at Thr-77 creates a low affinity degron also recognized by FBXW7.
Ubiquitinated by UHRF2; appears to occur independently of phosphorylation.

Sonntag, R., Penners, C., Kohlhepp, M., Haas, U., Lambertz, D., Kroh, A., ... & Liedtke, C. (2021). Cyclin E1 in Murine and Human Liver Cancer: A Promising Target for Therapeutic Intervention during Tumour Progression. Cancers, 13(22), 5680.

Pascual, J., Gil-Gil, M., Zielinski, C., Hills, M., Ruiz-Borrego, M., Ciruelos, E. M., ... & Martin, M. (2021). CCNE1 mRNA and cyclin E1 protein expression as predictive biomarkers for efficacy of palbociclib plus fulvestrant versus capecitabine in the phase III PEARL study.

Zhu, W., Si, Y., Xu, J., Lin, Y., Wang, J. Z., Cao, M., ... & Wei, J. F. (2020). Methyltransferase like 3 promotes colorectal cancer proliferation by stabilizing CCNE1 mRNA in an m6A‐dependent manner. Journal of cellular and molecular medicine, 24(6), 3521-3533.

Turner, N. C., Liu, Y., Zhu, Z., Loi, S., Colleoni, M., Loibl, S., ... & Cristofanilli, M. (2019). Cyclin E1 expression and palbociclib efficacy in previously treated hormone receptor–positive metastatic breast cancer. Journal of Clinical Oncology, 37(14), 1169.

Turner, N. C., Liu, Y., Zhu, Z., Loi, S., Colleoni, M., Loibl, S., ... & Cristofanilli, M. (2018). Abstract CT039: Cyclin E1 (CCNE1) expression associates with benefit from palbociclib in metastatic breast cancer (MBC) in the PALOMA3 trial.

Sonntag, R., Giebeler, N., Nevzorova, Y. A., Bangen, J. M., Fahrenkamp, D., Lambertz, D., ... & Liedtke, C. (2018). Cyclin E1 and cyclin-dependent kinase 2 are critical for initiation, but not for progression of hepatocellular carcinoma. Proceedings of the National Academy of Sciences, 115(37), 9282-9287.

Huang, X., & Lyu, J. (2018). Tumor suppressor function of miR‑483‑3p on breast cancer via targeting of the cyclin E1 gene. Experimental and therapeutic medicine, 16(3), 2615-2620.

Ehedego, H., Mohs, A., Jansen, B., Hiththetiya, K., Sicinski, P., Liedtke, C., & Trautwein, C. (2018). Loss of Cyclin E1 attenuates hepatitis and hepatocarcinogenesis in a mouse model of chronic liver injury. Oncogene, 37(25), 3329-3339.

Guarducci, C., Bonechi, M., Benelli, M., Biagioni, C., Boccalini, G., Romagnoli, D., ... & Migliaccio, I. (2018). Cyclin E1 and Rb modulation as common events at time of resistance to palbociclib in hormone receptor-positive breast cancer. NPJ Breast Cancer, 4(1), 1-10.

Bangen, J. M., Hammerich, L., Sonntag, R., Baues, M., Haas, U., Lambertz, D., ... & Liedtke, C. (2017). Targeting CCl4‐induced liver fibrosis by RNA interference–mediated inhibition of cyclin E1 in mice. Hepatology, 66(4), 1242-1257.

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For research use only. Not intended for any clinical use.

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