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Mouse Anti-CDC5L Recombinant Antibody (21/CDC5L) (CBMAB-C4363-LY)

This product is antibody recognizes CDC5L. The antibody 21/CDC5L immunoassay techniques such as: WB.
See all CDC5L antibodies

Summary

Host Animal
Mouse
Specificity
Rat, Chicken, Dog, Human, Mouse
Clone
21/CDC5L
Antibody Isotype
IgG1
Application
WB

Basic Information

Immunogen
Rat CDC5L aa. 109-303
Specificity
Rat, Chicken, Dog, Human, Mouse
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Concentration
0.25 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
CDC5L
Introduction
CDC5L (Cell Division Cycle 5 Like) is a Protein Coding gene. Diseases associated with CDC5L include Cystoisosporiasis and Renal Dysplasia. Among its related pathways are Gene Expression and mRNA Splicing - Major Pathway. Gene Ontology (GO) annotations related to this gene include chromatin binding.
Entrez Gene ID
Human988
Mouse71702
Rat85434
Chicken422052
Dog474921
UniProt ID
HumanQ99459
MouseQ6A068
RatO08837
ChickenE1C6A9
DogE2RLP4
Function
DNA-binding protein involved in cell cycle control. May act as a transcription activator. Plays role in pre-mRNA splicing as core component of precatalytic, catalytic and postcatalytic spliceosomal complexes (PubMed:11991638, PubMed:20176811, PubMed:28502770, PubMed:28076346, PubMed:29361316, PubMed:29360106, PubMed:29301961, PubMed:30728453, PubMed:30705154).
Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. The PRP19-CDC5L complex may also play a role in the response to DNA damage (DDR) (PubMed:20176811).
Biological Process
Cellular response to fibroblast growth factor stimulus Source: Ensembl
Cellular response to interleukin-2 Source: Ensembl
Cellular response to nerve growth factor stimulus Source: Ensembl
Cellular response to prolactin Source: Ensembl
Cellular response to wortmannin Source: Ensembl
DNA repair Source: UniProtKB-KW
Mitotic cell cycle Source: Ensembl
mRNA splicing, via spliceosome Source: UniProtKB
Positive regulation of transcription by RNA polymerase II Source: NTNU_SB
Regulation of transcription by RNA polymerase II Source: GO_Central
Signal transduction involved in DNA damage checkpoint Source: UniProtKB
Cellular Location
Nucleus; Nucleus speckle; Cytoplasm. May shuttle between cytoplasm and nucleus.
Involvement in disease
A chromosomal aberration involving CDC5L is found in multicystic renal dysplasia. Translocation t(6;19)(p21;q13.1) with USF2.
PTM
Phosphorylated on serine and threonine residues. Phosphorylation on Thr-411 and Thr-438 is required for CDC5L-mediated mRNA splicing. Has no effect on subcellular location nor on homodimerization. Phosphorylated in vitro by CDK2. Phosphorylation enhances interaction with PPP1R8.

Zhang, H. Y., Li, J., Ouyang, Y. C., Meng, T. G., Zhang, C. H., Yue, W., ... & Qian, W. P. (2021). Cell Division Cycle 5-Like Regulates Metaphase-to-Anaphase Transition in Meiotic Oocyte. Frontiers in Cell and Developmental Biology, 9.

Jokoji, G., Maeda, S., Oishi, K., Ijuin, T., Nakajima, M., Tawaratsumida, H., ... & Taniguchi, N. (2021). CDC5L promotes early chondrocyte differentiation and proliferation by modulating pre-mRNA splicing of SOX9, COL2A1, and WEE1. Journal of Biological Chemistry, 297(2).

Liu, H., Guo, D., Sha, Y., Zhang, C., Jiang, Y., Hong, L., ... & Huang, H. (2020). ANXA7 promotes the cell cycle, proliferation and cell adhesion-mediated drug resistance of multiple myeloma cells by up-regulating CDC5L. Aging (Albany NY), 12(11), 11100.

Zhang, C., Li, Y., Zhao, W., Liu, G., & Yang, Q. (2020). Circ‐PGAM1 promotes malignant progression of epithelial ovarian cancer through regulation of the miR‐542‐3p/CDC5L/PEAK1 pathway. Cancer medicine, 9(10), 3500-3521.

Zhang, Z., Mao, W., Wang, L., Liu, M., Zhang, W., Wu, Y., ... & Yao, X. (2020). Depletion of CDC5L inhibits bladder cancer tumorigenesis. Journal of Cancer, 11(2), 353.

Zhu, H., Tang, J. H., Zhang, S. M., Qian, J. P., Ling, X., Wu, X. Y., & Yang, L. X. (2020). Long Noncoding RNA LINC00963 Promotes CDC5L-Mediated Malignant Progression in Gastric Cancer. OncoTargets and therapy, 13, 12999.

Liu, X. M., Wang, Y. K., Liu, Y. H., Yu, X. X., Wang, P. C., Li, X., ... & Yang, C. X. (2018). Single-cell transcriptome sequencing reveals that cell division cycle 5-like protein is essential for porcine oocyte maturation. Journal of Biological Chemistry, 293(5), 1767-1780.

Li, X., Wang, X., Song, W., Xu, H., Huang, R., Wang, Y., ... & Yang, X. (2018). Oncogenic properties of NEAT1 in prostate cancer cells depend on the CDC5L–AGRN transcriptional regulation circuit. Cancer research, 78(15), 4138-4149.

Huang, R., Xue, R., Qu, D., Yin, J., & Shen, X. Z. (2017). Prp19 arrests cell cycle via Cdc5L in hepatocellular carcinoma cells. International journal of molecular sciences, 18(4), 778.

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For research use only. Not intended for any clinical use.

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