Mouse Anti-CDC5L Recombinant Antibody (CBFYC-1525) (CBMAB-C1584-FY)

Name: Mouse Anti-CDC5L Recombinant Antibody (CBFYC-1525)
SKU: CBMAB-C1584-FY
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-CDC5L Recombinant Antibody (CBFYC-1525) (CBMAB-C1584-FY) in WB

TSSC4 interacts with proteins specific for U5 snRNP and the PRPF19 complex. TSSC4-GFP immunoprecipitation followed by western blotting and detection of proteins specific for U5 (PRPF8, SNRNP200, EFTUD2, and PRPF6-top panel and DDX23 and TXNL4A-bottom panel), U2 (SNRPA1) and U4/U6 (PRPF31 and SART3) snRNPs and PRPF19 complex (PRPF19 and CDC5L). ...View More

Klimešová, K., Vojáčková, J., Radivojević, N., Vandermoere, F., Bertrand, E., Verheggen, C., & Staněk, D. (2021). TSSC4 is a component of U5 snRNP that promotes tri-snRNP formation. Nature Communications, 12(1), 3646.

Distributed under Open Access license CC BY 4.0, without modification.

Mouse Anti-CDC5L Recombinant Antibody (CBFYC-1525) (CBMAB-C1584-FY) in WB and IP

Co-immunoprecipitation of U5 and PRPF19 complex markers with TSSC4 deletion mutants. Deletion of aa 51-100 abolished interaction with several U5-specific proteins (PRPF6, SNRNP40, CD2BP2, and SNRPB). The same deletion reduced the association of TSSC4 with U5 proteins PRPF8, SNRNP200, and EFTUD2. Deletion of aa 201-250 specifically inhibited association with PRPF19 complex markers (bottom panel). ...View More

Distributed under Open Access license CC BY 4.0, without modification.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYC-1525

Application

WB, IF, IP, ELISA

Immunogen

Amino acids 503-802 mapping at the C-terminus of Cdc5L of human.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

CDC5L

Introduction

CDC5L (Cell Division Cycle 5 Like) is a Protein Coding gene. Diseases associated with CDC5L include Cystoisosporiasis and Renal Dysplasia. Among its related pathways are Gene Expression and mRNA Splicing-Major Pathway. Gene Ontology (GO) annotations related to this gene include chromatin binding.

Entrez Gene ID

988

UniProt ID

Q99459

Alternative Names

Cell Division Cycle 5 Like; Pombe Cdc5-Related Protein; PCDC5RP; CDC5 (Cell Division Cycle 5, S. Pombe, Homolog)-Like; CDC5 Cell Division Cycle 5-Like (S. Pombe); Cell Division Cycle 5-Like Protein; CDC5 Cell Division Cycle 5-Like; DJ319D22.1 (CDC5-Like Protein); Cell Division Cycle 5-Like

Function

DNA-binding protein involved in cell cycle control. May act as a transcription activator. Plays role in pre-mRNA splicing as core component of precatalytic, catalytic and postcatalytic spliceosomal complexes (PubMed:11991638, PubMed:20176811, PubMed:28502770, PubMed:28076346, PubMed:29361316, PubMed:29360106, PubMed:29301961, PubMed:30728453, PubMed:30705154).
Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. The PRP19-CDC5L complex may also play a role in the response to DNA damage (DDR) (PubMed:20176811).

Biological Process

Cellular response to fibroblast growth factor stimulus Source: Ensembl
Cellular response to interleukin-2 Source: Ensembl
Cellular response to nerve growth factor stimulus Source: Ensembl
Cellular response to prolactin Source: Ensembl
Cellular response to wortmannin Source: Ensembl
DNA repair Source: UniProtKB-KW
Mitotic cell cycle Source: Ensembl
mRNA splicing, via spliceosome Source: UniProtKB
Positive regulation of transcription by RNA polymerase II Source: NTNU_SB
Regulation of transcription by RNA polymerase II Source: GO_Central
Signal transduction involved in DNA damage checkpoint Source: UniProtKB

Cellular Location

Nucleus; Nucleus speckle; Cytoplasm. May shuttle between cytoplasm and nucleus.

Involvement in disease

A chromosomal aberration involving CDC5L is found in multicystic renal dysplasia. Translocation t(6;19)(p21;q13.1) with USF2.

PTM

Phosphorylated on serine and threonine residues. Phosphorylation on Thr-411 and Thr-438 is required for CDC5L-mediated mRNA splicing. Has no effect on subcellular location nor on homodimerization. Phosphorylated in vitro by CDK2. Phosphorylation enhances interaction with PPP1R8.

Zhang, H. Y., Li, J., Ouyang, Y. C., Meng, T. G., Zhang, C. H., Yue, W., ... & Qian, W. P. (2021). Cell Division Cycle 5-Like Regulates Metaphase-to-Anaphase Transition in Meiotic Oocyte. Frontiers in Cell and Developmental Biology, 9.

Jokoji, G., Maeda, S., Oishi, K., Ijuin, T., Nakajima, M., Tawaratsumida, H., ... & Taniguchi, N. (2021). CDC5L promotes early chondrocyte differentiation and proliferation by modulating pre-mRNA splicing of SOX9, COL2A1, and WEE1. Journal of Biological Chemistry, 297(2).

Liu, H., Guo, D., Sha, Y., Zhang, C., Jiang, Y., Hong, L., ... & Huang, H. (2020). ANXA7 promotes the cell cycle, proliferation and cell adhesion-mediated drug resistance of multiple myeloma cells by up-regulating CDC5L. Aging (Albany NY), 12(11), 11100.

Zhang, C., Li, Y., Zhao, W., Liu, G., & Yang, Q. (2020). Circ‐PGAM1 promotes malignant progression of epithelial ovarian cancer through regulation of the miR‐542‐3p/CDC5L/PEAK1 pathway. Cancer medicine, 9(10), 3500-3521.

Zhang, Z., Mao, W., Wang, L., Liu, M., Zhang, W., Wu, Y., ... & Yao, X. (2020). Depletion of CDC5L inhibits bladder cancer tumorigenesis. Journal of Cancer, 11(2), 353.

Zhu, H., Tang, J. H., Zhang, S. M., Qian, J. P., Ling, X., Wu, X. Y., & Yang, L. X. (2020). Long Noncoding RNA LINC00963 Promotes CDC5L-Mediated Malignant Progression in Gastric Cancer. OncoTargets and therapy, 13, 12999.

Liu, X. M., Wang, Y. K., Liu, Y. H., Yu, X. X., Wang, P. C., Li, X., ... & Yang, C. X. (2018). Single-cell transcriptome sequencing reveals that cell division cycle 5-like protein is essential for porcine oocyte maturation. Journal of Biological Chemistry, 293(5), 1767-1780.

Li, X., Wang, X., Song, W., Xu, H., Huang, R., Wang, Y., ... & Yang, X. (2018). Oncogenic properties of NEAT1 in prostate cancer cells depend on the CDC5L–AGRN transcriptional regulation circuit. Cancer research, 78(15), 4138-4149.

Huang, R., Xue, R., Qu, D., Yin, J., & Shen, X. Z. (2017). Prp19 arrests cell cycle via Cdc5L in hepatocellular carcinoma cells. International journal of molecular sciences, 18(4), 778.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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For research use only. Not intended for any clinical use.

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