Rabbit Anti-CDC5L Recombinant Antibody (
1E3) (V2LY-0725-LY1413)

Name: Rabbit Anti-CDC5L Recombinant Antibody (1E3)
SKU: V2LY-0725-LY1413
Rating: 5 (1 reviews)

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Tested Data

Rabbit Anti-CDC5L Recombinant Antibody (1E3) (V2LY-0725-LY1413) in WB

Western Blot
Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate, 293 whole cell lysate, K562 whole cell lysate, Jurkat whole cell lysate
All lanes: CDC5L antibody at 1.3μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 93 KDa
Observed band size: 100 KDa

Rabbit Anti-CDC5L Recombinant Antibody (1E3) (V2LY-0725-LY1413) in IHC

IHC image of V2LY-0725-LY1413 diluted at 1:126.5 and staining in paraffin-embedded human appendix tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Rabbit Anti-CDC5L Recombinant Antibody (1E3) (V2LY-0725-LY1413) in IHC

IHC image of V2LY-0725-LY1413 diluted at 1:126.5 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Rabbit Anti-CDC5L Recombinant Antibody (1E3) (V2LY-0725-LY1413) in IF

Immunofluorescence staining of A549 cells with V2LY-0725-LY1413 at 1:42, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Rabbit Anti-CDC5L Recombinant Antibody (1E3) (V2LY-0725-LY1413) in IP

Immunoprecipitating CDC5L in Hela whole cell lysate
Lane 1: Rabbit control IgG instead of V2LY-0725-LY1413 in Hela whole cell lysate.
For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: V2LY-0725-LY1413 (3μg) + Hela whole cell lysate (500μg)
Lane 3: Hela whole cell lysate (20μg)

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

1E3

Application

ELISA, WB, IHC, IF, IP

Immunogen

A synthesized peptide derived from human CDC5L.

Host Species

Rabbit

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:5,000
IHC	1:50-1:200
IF	1:20-1:200
IP	1:200-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

CDC5L

Entrez Gene ID

988

UniProt ID

Q99459

Function

DNA-binding protein involved in cell cycle control. May act as a transcription activator. Plays role in pre-mRNA splicing as core component of precatalytic, catalytic and postcatalytic spliceosomal complexes (PubMed:11991638, PubMed:20176811, PubMed:28502770, PubMed:28076346, PubMed:29361316, PubMed:29360106, PubMed:29301961, PubMed:30728453, PubMed:30705154).
Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. The PRP19-CDC5L complex may also play a role in the response to DNA damage (DDR) (PubMed:20176811).

Biological Process

Cellular response to fibroblast growth factor stimulus Source: Ensembl
Cellular response to interleukin-2 Source: Ensembl
Cellular response to nerve growth factor stimulus Source: Ensembl
Cellular response to prolactin Source: Ensembl
Cellular response to wortmannin Source: Ensembl
DNA repair Source: UniProtKB-KW
Mitotic cell cycle Source: Ensembl
mRNA splicing, via spliceosome Source: UniProtKB
Positive regulation of transcription by RNA polymerase II Source: NTNU_SB
Regulation of transcription by RNA polymerase II Source: GO_Central
Signal transduction involved in DNA damage checkpoint Source: UniProtKB

Cellular Location

Nucleus; Nucleus speckle; Cytoplasm. May shuttle between cytoplasm and nucleus.

Involvement in disease

A chromosomal aberration involving CDC5L is found in multicystic renal dysplasia. Translocation t(6;19)(p21;q13.1) with USF2.

PTM

Phosphorylated on serine and threonine residues. Phosphorylation on Thr-411 and Thr-438 is required for CDC5L-mediated mRNA splicing. Has no effect on subcellular location nor on homodimerization. Phosphorylated in vitro by CDK2. Phosphorylation enhances interaction with PPP1R8.

Zhang, H. Y., Li, J., Ouyang, Y. C., Meng, T. G., Zhang, C. H., Yue, W., ... & Qian, W. P. (2021). Cell Division Cycle 5-Like Regulates Metaphase-to-Anaphase Transition in Meiotic Oocyte. Frontiers in Cell and Developmental Biology, 9.

Jokoji, G., Maeda, S., Oishi, K., Ijuin, T., Nakajima, M., Tawaratsumida, H., ... & Taniguchi, N. (2021). CDC5L promotes early chondrocyte differentiation and proliferation by modulating pre-mRNA splicing of SOX9, COL2A1, and WEE1. Journal of Biological Chemistry, 297(2).

Liu, H., Guo, D., Sha, Y., Zhang, C., Jiang, Y., Hong, L., ... & Huang, H. (2020). ANXA7 promotes the cell cycle, proliferation and cell adhesion-mediated drug resistance of multiple myeloma cells by up-regulating CDC5L. Aging (Albany NY), 12(11), 11100.

Zhang, C., Li, Y., Zhao, W., Liu, G., & Yang, Q. (2020). Circ‐PGAM1 promotes malignant progression of epithelial ovarian cancer through regulation of the miR‐542‐3p/CDC5L/PEAK1 pathway. Cancer medicine, 9(10), 3500-3521.

Zhang, Z., Mao, W., Wang, L., Liu, M., Zhang, W., Wu, Y., ... & Yao, X. (2020). Depletion of CDC5L inhibits bladder cancer tumorigenesis. Journal of Cancer, 11(2), 353.

Zhu, H., Tang, J. H., Zhang, S. M., Qian, J. P., Ling, X., Wu, X. Y., & Yang, L. X. (2020). Long Noncoding RNA LINC00963 Promotes CDC5L-Mediated Malignant Progression in Gastric Cancer. OncoTargets and therapy, 13, 12999.

Liu, X. M., Wang, Y. K., Liu, Y. H., Yu, X. X., Wang, P. C., Li, X., ... & Yang, C. X. (2018). Single-cell transcriptome sequencing reveals that cell division cycle 5-like protein is essential for porcine oocyte maturation. Journal of Biological Chemistry, 293(5), 1767-1780.

Li, X., Wang, X., Song, W., Xu, H., Huang, R., Wang, Y., ... & Yang, X. (2018). Oncogenic properties of NEAT1 in prostate cancer cells depend on the CDC5L–AGRN transcriptional regulation circuit. Cancer research, 78(15), 4138-4149.

Huang, R., Xue, R., Qu, D., Yin, J., & Shen, X. Z. (2017). Prp19 arrests cell cycle via Cdc5L in hepatocellular carcinoma cells. International journal of molecular sciences, 18(4), 778.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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For research use only. Not intended for any clinical use.

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