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Mouse Anti-CERS6 Recombinant Antibody (CBYJL-105) (CBMAB-L0161-YJ)

Provided herein is a Mouse monoclonal antibody, which binds to Ceramide Synthase 6 (CERS6). The antibody can be used for immunoassay techniques, such as WB, ELISA.
See all CERS6 antibodies

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
CBYJL-105
Antibody Isotype
IgG2b
Application
WB, ELISA

Basic Information

Specificity
Human, Mouse, Rat
Antibody Isotype
IgG2b
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
PBS, pH 7.4
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Ceramide Synthase 6
Introduction
Among CERS6's related pathways are Metabolism and Sphingolipid metabolism. Gene Ontology (GO) annotations related to this gene include sphingosine N-acyltransferase activity. CERS5 is an important paralog of this gene.
Entrez Gene ID
Human253782
Mouse241447
Rat366065
UniProt ID
HumanQ6ZMG9
MouseQ8C172
RatF1LTP8
Alternative Names
Ceramide Synthase 6; LAG1 Homolog; Ceramide Synthase 6; LASS6; LAG1 Longevity Assurance Homolog 6 (S. Cerevisiae); LAG1 Longevity Assurance Homolog 6; Longevity Assurance Homolog 6; CerS5; CerS6
Function
Ceramide synthase that catalyzes formation of ceramide from sphinganine and acyl-CoA substrates, with high selectivity toward palmitoyl-CoA (hexadecanoyl-CoA; C16:0-CoA) as acyl donor (PubMed:17977534, PubMed:17609214, PubMed:23530041, PubMed:26887952, PubMed:31916624).
Can use other acyl donors, but with less efficiency (By similarity).
Ceramides generated by CERS6 play a role in inflammatory response (By similarity).
Acts as a regulator of metabolism and hepatic lipid accumulation (By similarity).
Under high fat diet, palmitoyl- (C16:0-) ceramides generated by CERS6 specifically bind the mitochondrial fission factor MFF, thereby promoting mitochondrial fragmentation and contributing to the development of obesity (By similarity).
Biological Process
Ceramide biosynthetic process Source: UniProtKB
Inflammatory response Source: UniProtKB-KW
Sphingolipid biosynthetic process Source: Reactome
Cellular Location
Endoplasmic reticulum membrane
Topology
Lumenal: 1-34
Helical: 35-55
Helical: 178-198
Helical: 205-225
Helical: 263-283
Helical: 303-323
Cytoplasmic: 324-384
PTM
Phosphorylated at the C-terminus by CK2.
Acetylated. Deacetylation by SIRT3 increases enzyme activity and promotes mitochondrial ceramide accumulation.

Barron, K., Ogretmen, B., & Krupenko, N. (2021). Ceramide synthase 6 mediates sex-specific metabolic response to dietary folic acid in mice. The Journal of Nutritional Biochemistry, 98, 108832.

Lu, P., White-Gilbertson, S., Beeson, G., Beeson, C., Ogretmen, B., Norris, J., & Voelkel-Johnson, C. (2021). Ceramide Synthase 6 Maximizes p53 Function to Prevent Progeny Formation from Polyploid Giant Cancer Cells. Cancers, 13(9), 2212.

Shi, Y., Zhou, C., Lu, H., Cui, X., Li, J., Jiang, S., ... & Zhang, R. (2020). Ceramide synthase 6 predicts poor prognosis and activates the AKT/mTOR/4EBP1 pathway in high-grade serous ovarian cancer. American Journal of Translational Research, 12(9), 5924.

Holmes, R. S., Barron, K. A., & Krupenko, N. I. (2018). Ceramide synthase 6: Comparative analysis, phylogeny and evolution. Biomolecules, 8(4), 111.

Williams, B., Correnti, J., Oranu, A., Lin, A., Scott, V., Annoh, M., ... & Carr, R. M. (2018). A novel role for ceramide synthase 6 in mouse and human alcoholic steatosis. The FASEB Journal, 32(1), 130-142.

Helke, K., Angel, P., Lu, P., Garrett-Mayer, E., Ogretmen, B., Drake, R., & Voelkel-Johnson, C. (2018). Ceramide synthase 6 deficiency enhances inflammation in the DSS model of colitis. Scientific reports, 8(1), 1-9.

Verlekar, D., Wei, S. J., Cho, H., Yang, S., & Kang, M. H. (2018). Ceramide synthase-6 confers resistance to chemotherapy by binding to CD95/Fas in T-cell acute lymphoblastic leukemia. Cell death & disease, 9(9), 1-14.

Uen, Y. H., Fang, C. L., Lin, C. C., Hseu, Y. C., Hung, S. T., Sun, D. P., & Lin, K. Y. (2018). Ceramide synthase 6 predicts the prognosis of human gastric cancer: it functions as an oncoprotein by dysregulating the SOCS2/JAK2/STAT3 pathway. Molecular carcinogenesis, 57(12), 1675-1689.

Scheffel, M. J., Helke, K., Lu, P., Bowers, J. S., Ogretmen, B., Garrett-Mayer, E., ... & Voelkel-Johnson, C. (2017). Adoptive transfer of ceramide synthase 6 deficient splenocytes reduces the development of colitis. Scientific reports, 7(1), 1-10.

Kim, M. H., Ahn, H. K., Lee, E. J., Kim, S. J., Kim, Y. R., Park, J. W., & Park, W. J. (2017). Hepatic inflammatory cytokine production can be regulated by modulating sphingomyelinase and ceramide synthase 6. International journal of molecular medicine, 39(2), 453-462.

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For research use only. Not intended for any clinical use.

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