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Rabbit Anti-CLDN7 Recombinant Antibody (EG725) (CBMAB-EN879-LY)

The product is antibody recognizes CLDN7. The antibody EG725 immunoassay techniques such as: WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000.
See all CLDN7 antibodies

Summary

Host Animal
Rabbit
Specificity
Human, Mouse, Rat
Clone
EG725
Antibody Isotype
IgG
Application
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000

Basic Information

Immunogen
The antibody was produced against synthesized non-phosphopeptide derived from human Claudin 7 around the phosphorylation site of tyrosine 210 (S-K-E-YP-V).
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Claudin 7
Introduction
This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. Differential expression of this gene has been observed in different types of malignancies, including breast cancer, ovarian cancer, hepatocellular carcinomas, urinary tumors, prostate cancer, lung cancer, head and neck cancers, thyroid carcinomas, etc. Alternatively spliced transcript variants encoding different isoforms have been found.[provided by RefSeq, May 2010]
Entrez Gene ID
Human1366
Mouse53624
Rat65132
UniProt ID
HumanO95471
MouseQ9Z261
RatQ9Z1L1
Alternative Names
Claudin 7; CEPTRL2; CPETRL2; CLDN-7; Clostridium Perfringens Enterotoxin Receptor-Like 2; Claudin-1; Claudin-7; Hs.84359;
Function
Plays a major role in tight junction-specific obliteration of the intercellular space.
Biological Process
Bicellular tight junction assembly Source: GO_Central
Calcium-independent cell-cell adhesion via plasma membrane cell-adhesion molecules Source: UniProtKB
Cell adhesion Source: GO_Central
Negative regulation of apoptotic process Source: Ensembl
Negative regulation of cell adhesion Source: Ensembl
Negative regulation of protein-containing complex assembly Source: Ensembl
Positive regulation of cell motility Source: Ensembl
Positive regulation of cell population proliferation Source: Ensembl
Response to ethanol Source: Ensembl
Cellular Location
Cell membrane; Basolateral cell membrane; Tight junction. Co-localizes with EPCAM at the basolateral cell membrane and tight junction.
Topology
Cytoplasmic: 1-7
Helical: 8-28
Extracellular: 29-81
Helical: 82-102
Cytoplasmic: 103-117
Helical: 118-138
Extracellular: 139-160
Helical: 161-181
Cytoplasmic: 182-221
PTM
Phosphorylated.

Ding, Y., Wang, K., Xu, C., Hao, M., Li, H., & Ding, L. (2022). Intestinal Claudin-7 deficiency impacts the intestinal microbiota in mice with colitis. BMC gastroenterology, 22(1), 1-17.

Li, X., & Yang, W. (2022). IRF2‑induced Claudin‑7 suppresses cell proliferation, invasion and migration of oral squamous cell carcinoma. Experimental and Therapeutic Medicine, 23(1), 1-9.

Xu, C., Ding, Y. H., Wang, K., Hao, M., Li, H., & Ding, L. (2021). Claudin-7 deficiency promotes stemness properties in colorectal cancer through Sox9-mediated Wnt/β-catenin signalling. Journal of Translational Medicine, 19(1), 1-15.

Xing, T., Benderman, L. J., Sabu, S., Parker, J., Yang, J., Lu, Q., ... & Chen, Y. H. (2020). Tight junction protein claudin-7 is essential for intestinal epithelial stem cell self-renewal and differentiation. Cellular and molecular gastroenterology and hepatology, 9(4), 641-659.

Ji, H., Ding, X., Zhang, W., Zheng, Y., Du, H., Zheng, Y., ... & Zhong, Y. (2020). Claudin-7 Inhibits Proliferation and Metastasis in Salivary Adenoid Cystic Carcinoma Through Wnt/β-Catenin Signaling. Cell Transplantation, 29, 0963689720943583.

Xu, C., Wang, K., Ding, Y. H., Li, W. J., & Ding, L. (2019). Claudin-7 gene knockout causes destruction of intestinal structure and animal death in mice. World journal of gastroenterology, 25(5), 584.

Fan, J., Tatum, R., Hoggard, J., & Chen, Y. H. (2019). Claudin-7 modulates cl− and na+ homeostasis and wnk4 expression in renal collecting duct cells. International journal of molecular sciences, 20(15), 3798.

Nguyen, N., Fernando, S. D., Biette, K. A., Hammer, J. A., Capocelli, K. E., Kitzenberg, D. A., ... & Masterson, J. C. (2018). TGF-β1 alters esophageal epithelial barrier function by attenuation of claudin-7 in eosinophilic esophagitis. Mucosal immunology, 11(2), 415-426.

Wu, Z., Shi, J., Song, Y., Zhao, J., Sun, J., Chen, X., ... & Wang, Z. (2018). Claudin-7 (CLDN7) is overexpressed in gastric cancer and promotes gastric cancer cell proliferation, invasion and maintains mesenchymal state. Neoplasma, 65(3), 349-359.

Li, W. J., Xu, C., Wang, K., Li, T. Y., Wang, X. N., Yang, H., ... & Ding, L. (2018). Severe intestinal inflammation in the small intestine of mice induced by controllable deletion of claudin-7. Digestive diseases and sciences, 63(5), 1200-1209.

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For research use only. Not intended for any clinical use.

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