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Mouse Anti-CLIC4 Recombinant Antibody (CBFYC-1943) (V2LY-1206-LY225)


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Published Data
V2LY-1206-LY225-1
Mouse Anti-CLIC4 Recombinant Antibody (CBFYC-1943) (V2LY-1206-LY225) in WB
Analysis of the expression of CD146/MCAM, Clic4, and Glypican4 in EVs isolated from shControl, shRalA and shRalB cells by western blots. ...View More
Ghoroghi, S., Mary, B., Larnicol, A., Asokan, N., Klein, A., Osmani, N., ... & Hyenne, V. (2021). Ral GTPases promote breast cancer metastasis by controlling biogenesis and organ targeting of exosomes. Elife, 10, e61539.
Distributed under Open Access license CC BY 4.0, without modification.
V2LY-1206-LY225-2
Mouse Anti-CLIC4 Recombinant Antibody (CBFYC-1943) (V2LY-1206-LY225) in WB
mRNA changes of cell-cell adhesion and angiogenesis pathway genes from RNA-seq results in HUVECs, the significantly changed genes were also examined by Western Blotting. ...View More
Wang, K., Chen, H., Zhou, Z., Zhang, H., Zhou, H. J., & Min, W. (2021). ATPIF1 maintains normal mitochondrial structure which is impaired by CCM3 deficiency in endothelial cells. Cell & Bioscience, 11, 1-15.
Distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
V2LY-1206-LY225-3
Mouse Anti-CLIC4 Recombinant Antibody (CBFYC-1943) (V2LY-1206-LY225) in IF
Co-localization of endogenous CLIC4 with anillin, ALIX, and ezrin in cytokinesis. Maximum intensity projections of Z-stacks show endogenous anillin, ALIX, and ezrin (red), endogenous CLIC4 (green), and DNA staining (DAPI, blue). Scale bars, 5 μm. ...View More
Kagiali, Z. C. U., Saner, N., Akdag, M., Sanal, E., Degirmenci, B. S., Mollaoglu, G., & Ozlu, N. (2020). CLIC4 and CLIC1 bridge plasma membrane and cortical actin network for a successful cytokinesis. Life science alliance, 3(2).
Distributed under Open Access license CC BY 4.0, without modification.
V2LY-1206-LY225-4
Mouse Anti-CLIC4 Recombinant Antibody (CBFYC-1943) (V2LY-1206-LY225) in WB
(i) CLIC1 and CLIC4, respectively, as well as (ii) CLIC1 and CLIC4 in combination, were knocked down by transfecting siRNA into Huh7 cells. ...View More
Müller, M., Slivinski, N., Todd, E. J., Khalid, H., Li, R., Karwatka, M., ... & Tuplin, A. (2019). Chikungunya virus requires cellular chloride channels for efficient genome replication. PLoS neglected tropical diseases, 13(9), e0007703.
Distributed under Open Access license CC BY 4.0, without modification.
V2LY-1206-LY225-5
Mouse Anti-CLIC4 Recombinant Antibody (CBFYC-1943) (V2LY-1206-LY225) in IF
Confocal microscopy analysis of unstimulated (UT) or LPS-stimulated BMDMs stained with phalloidin, DAPI, and CLIC1 or CLIC4 antibodies followed by an Alexa-488-conjugated secondary antibody. Merged signals are shown in the right panels. ...View More
Domingo-Fernández, R., Coll, R. C., Kearney, J., Breit, S., & O'Neill, L. A. (2017). The intracellular chloride channel proteins CLIC1 and CLIC4 induce IL-1β transcription and activate the NLRP3 inflammasome. Journal of Biological Chemistry, 292(29), 12077-12087.
Distributed under Open Access license CC BY 4.0, without modification.
V2LY-1206-LY225-6
Mouse Anti-CLIC4 Recombinant Antibody (CBFYC-1943) (V2LY-1206-LY225) in IF
IF-staining for CLIC1, CLIC4, and CLIC5 in the atrial tissue. DAPI for nuclear; Alexa Fluor 488 for detecting CLIC4, Alexa Fluor 594 for detecting CLIC5 and Alexa Fluor 633 for detecting CLIC1, far red light was adjusted to yellow one to distinguish with red light. ...View More
Jiang, Y. Y., Hou, H. T., Yang, Q., Liu, X. C., & He, G. W. (2017). Chloride channels are involved in the development of atrial fibrillation-A transcriptomic and proteomic study. Scientific reports, 7(1), 10215.
Distributed under Open Access license CC BY 4.0, without modification.
V2LY-1206-LY225-7
Mouse Anti-CLIC4 Recombinant Antibody (CBFYC-1943) (V2LY-1206-LY225) in WB and IP
Clics are important for NLRP3 inflammasome assembly. a Immunoblot analysis of ASC oligomerization in lysates of LPS-primed Clic4 -/- BMDMs transfected with siRNA against Clic1 and Clic5 and left stimulated with ATP or nigericin. b Immunoprecipitation (IP) and immunoblot analysis of the interaction of endogenous NLRP3 and ASC in LPS-primed Clic4 -/- BMDMs transfected with siRNA against Clic1 and Clic5, and left stimulated with ATP or nigericin. c IP and immunoblot analysis of the interaction of endogenous NLRP3 and NEK7 in LPS-primed Clic4 -/- BMDMs transfected with siRNA against Clic1 and Clic5, and left stimulated with ATP or nigericin. d Model for the function and mechanism of Clics and chloride efflux in NLRP3 inflammasome activation. Data are representative of three independent experiments a-c. ...View More
Tang, T., Lang, X., Xu, C., Wang, X., Gong, T., Yang, Y., ... & Zhou, R. (2017). CLICs-dependent chloride efflux is an essential and proximal upstream event for NLRP3 inflammasome activation. Nature communications, 8(1), 202.
Distributed under Open Access license CC BY 4.0, without modification.
V2LY-1206-LY225-8
Mouse Anti-CLIC4 Recombinant Antibody (CBFYC-1943) (V2LY-1206-LY225) in ELISA
ELISA of IL-1β in supernatants of LPS-primed Clic4 -/- BMDMs transfected siRNA against Clic1 and Clic5 and left stimulated with nigericin for 30 min or transferred to NaCl, NaBr or NaI saline for 3 h. ...View More
Tang, T., Lang, X., Xu, C., Wang, X., Gong, T., Yang, Y., ... & Zhou, R. (2017). CLICs-dependent chloride efflux is an essential and proximal upstream event for NLRP3 inflammasome activation. Nature communications, 8(1), 202.
Distributed under Open Access license CC BY 4.0, without modification.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
CBFYC-1943
Antibody Isotype
IgG1, κ
Application
WB, IP, IF, ELISA, IHC-P

Basic Information

Immunogen
Amino acids 238-250 of CLIC4 of human.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1, κ
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000
IHC-P1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
0.1% gelatin
Preservative
<0.1% sodium azide
Concentration
0.2 mg/ml
Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Chloride Intracellular Channel 4
Entrez Gene ID
Human25932
Mouse29876
Rat83718
UniProt ID
HumanQ9Y696
MouseQ9QYB1
RatQ9Z0W7
Function
Can insert into membranes and form poorly selective ion channels that may also transport chloride ions. Channel activity depends on the pH. Membrane insertion seems to be redox-regulated and may occur only under oxydizing conditions. Promotes cell-surface expression of HRH3. Has alternate cellular functions like a potential role in angiogenesis or in maintaining apical-basolateral membrane polarity during mitosis and cytokinesis. Could also promote endothelial cell proliferation and regulate endothelial morphogenesis (tubulogenesis).
Biological Process
Angiogenesis Source: GO_Central
Branching morphogenesis of an epithelial tube Source: Ensembl
Cell differentiation Source: UniProtKB
Cellular response to calcium ion Source: UniProtKB
Chloride transport Source: UniProtKB
Endothelial cell morphogenesis Source: Ensembl
Establishment or maintenance of apical/basal cell polarity Source: UniProtKB
Fertilization Source: Ensembl
Keratinocyte differentiation Source: UniProtKB
Multicellular organism growth Source: Ensembl
Negative regulation of cell migration Source: UniProtKB
Regulation of cytoskeleton organization Source: UniProtKB
Regulation of ion transmembrane transport Source: UniProtKB-KW
Retina vasculature morphogenesis in camera-type eye Source: Ensembl
Vacuolar acidification Source: Ensembl
Cellular Location
Cell membrane; Nucleus matrix; Centrosome; Mitochondrion; Cytoplasmic vesicle membrane; Cell junction. Colocalized with AKAP9 at the centrosome and midbody. Exists both as soluble cytoplasmic protein and as membrane protein with probably a single transmembrane domain. Present in an intracellular vesicular compartment that likely represent trans-Golgi network vesicles.
Topology
Helical: 37-57
More Infomation

Zhou, W., Menkhorst, E., & Dimitriadis, E. (2022). Characterization of chloride intracellular channel 4 in the regulation of human trophoblast function. Placenta.

Bogenpohl, J. W., Weston, R. M., Foreman, T. N., Kitchen, K. E., & Miles, M. F. (2021). Chloride intracellular channel 4 (CLIC4) expression profile in the mouse medial prefrontal cortex and its regulation by ethanol. Alcoholism: Clinical and Experimental Research.

Chiang, P. C., Li, P. T., Lee, M. J., & Chen, C. T. (2021). DNA Hypermethylation Involves in the Down-Regulation of Chloride Intracellular Channel 4 (CLIC4) Induced by Photodynamic Therapy. Biomedicines, 9(8), 927.

Wasson, C. W., Ross, R. L., Morton, R., Mankouri, J., & Del Galdo, F. (2021). The intracellular chloride channel 4 (CLIC4) activates systemic sclerosis fibroblasts. Rheumatology, 60(9), 4395-4400.

Ling, C. K., Santos, L. L., Zhou, W., & Dimitriadis, E. (2020). Chloride intracellular channel 4 is dysregulated in endometrium of women with infertility and alters receptivity. Biochemical and Biophysical Research Communications, 531(4), 490-496.

Li, B., Zhao, Y., Song, M., Cui, H., Feng, X., Yang, T., & Fan, H. G. (2020). Role of c-Myc/chloride intracellular channel 4 pathway in lipopolysaccharide-induced neurodegenerative diseases. Toxicology, 429, 152312.

Feng, Z., Zhu, Z., Chen, W., Bai, Y., Hu, D., & Cheng, J. (2020). Chloride intracellular channel 4 participate in the protective effect of Ginkgolide B in MPP+ injured MN9D cells: insight from proteomic analysis. Clinical Proteomics, 17(1), 1-10.

Huang, S., Huang, Z., Chen, P., & Feng, C. (2020). Aberrant chloride intracellular channel 4 expression is associated with adverse outcome in cytogenetically normal acute myeloid leukemia. Frontiers in oncology, 10, 1648.

Guo, D., Xie, W., Xiong, P., Li, H., Wang, S., Chen, G., ... & Zhang, J. (2018). Cyclin-dependent kinase 5-mediated phosphorylation of chloride intracellular channel 4 promotes oxidative stress-induced neuronal death. Cell death & disease, 9(10), 1-13.

Argenzio, E., Klarenbeek, J., Kedziora, K. M., Nahidiazar, L., Isogai, T., Perrakis, A., ... & Innocenti, M. (2018). Profilin binding couples chloride intracellular channel protein CLIC4 to RhoA–mDia2 signaling and filopodium formation. Journal of Biological Chemistry, 293(50), 19161-19176.

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For research use only. Not intended for any clinical use.

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