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Mouse Anti-CLIC4 Recombinant Antibody (CBXC-2485) (V2LY-1206-LY226)


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Summary

Host Animal
Mouse
Specificity
Human, Pig, Rat
Clone
CBXC-2485
Antibody Isotype
IgG1
Application
IF, WB, IHC

Basic Information

Immunogen
CLIC4 Fusion Protein (1-253 aa).
Host Species
Mouse
Specificity
Human, Pig, Rat
Antibody Isotype
IgG1
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
WB1:1,000-1:4,000
IHC-P1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.3, 50% glycerol
Preservative
0.02% sodium azide
Concentration
1 mg/ml
Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Chloride Intracellular Channel 4
Entrez Gene ID
Human25932Rat
UniProt ID
HumanQ9Y696Rat
Function
Can insert into membranes and form poorly selective ion channels that may also transport chloride ions. Channel activity depends on the pH. Membrane insertion seems to be redox-regulated and may occur only under oxydizing conditions. Promotes cell-surface expression of HRH3. Has alternate cellular functions like a potential role in angiogenesis or in maintaining apical-basolateral membrane polarity during mitosis and cytokinesis. Could also promote endothelial cell proliferation and regulate endothelial morphogenesis (tubulogenesis).
Biological Process
Angiogenesis Source: GO_Central
Branching morphogenesis of an epithelial tube Source: Ensembl
Cell differentiation Source: UniProtKB
Cellular response to calcium ion Source: UniProtKB
Chloride transport Source: UniProtKB
Endothelial cell morphogenesis Source: Ensembl
Establishment or maintenance of apical/basal cell polarity Source: UniProtKB
Fertilization Source: Ensembl
Keratinocyte differentiation Source: UniProtKB
Multicellular organism growth Source: Ensembl
Negative regulation of cell migration Source: UniProtKB
Regulation of cytoskeleton organization Source: UniProtKB
Regulation of ion transmembrane transport Source: UniProtKB-KW
Retina vasculature morphogenesis in camera-type eye Source: Ensembl
Vacuolar acidification Source: Ensembl
Cellular Location
Cell membrane; Nucleus matrix; Centrosome; Mitochondrion; Cytoplasmic vesicle membrane; Cell junction. Colocalized with AKAP9 at the centrosome and midbody. Exists both as soluble cytoplasmic protein and as membrane protein with probably a single transmembrane domain. Present in an intracellular vesicular compartment that likely represent trans-Golgi network vesicles.
Topology
Helical: 37-57
More Infomation

Zhou, W., Menkhorst, E., & Dimitriadis, E. (2022). Characterization of chloride intracellular channel 4 in the regulation of human trophoblast function. Placenta.

Bogenpohl, J. W., Weston, R. M., Foreman, T. N., Kitchen, K. E., & Miles, M. F. (2021). Chloride intracellular channel 4 (CLIC4) expression profile in the mouse medial prefrontal cortex and its regulation by ethanol. Alcoholism: Clinical and Experimental Research.

Chiang, P. C., Li, P. T., Lee, M. J., & Chen, C. T. (2021). DNA Hypermethylation Involves in the Down-Regulation of Chloride Intracellular Channel 4 (CLIC4) Induced by Photodynamic Therapy. Biomedicines, 9(8), 927.

Wasson, C. W., Ross, R. L., Morton, R., Mankouri, J., & Del Galdo, F. (2021). The intracellular chloride channel 4 (CLIC4) activates systemic sclerosis fibroblasts. Rheumatology, 60(9), 4395-4400.

Ling, C. K., Santos, L. L., Zhou, W., & Dimitriadis, E. (2020). Chloride intracellular channel 4 is dysregulated in endometrium of women with infertility and alters receptivity. Biochemical and Biophysical Research Communications, 531(4), 490-496.

Li, B., Zhao, Y., Song, M., Cui, H., Feng, X., Yang, T., & Fan, H. G. (2020). Role of c-Myc/chloride intracellular channel 4 pathway in lipopolysaccharide-induced neurodegenerative diseases. Toxicology, 429, 152312.

Feng, Z., Zhu, Z., Chen, W., Bai, Y., Hu, D., & Cheng, J. (2020). Chloride intracellular channel 4 participate in the protective effect of Ginkgolide B in MPP+ injured MN9D cells: insight from proteomic analysis. Clinical Proteomics, 17(1), 1-10.

Huang, S., Huang, Z., Chen, P., & Feng, C. (2020). Aberrant chloride intracellular channel 4 expression is associated with adverse outcome in cytogenetically normal acute myeloid leukemia. Frontiers in oncology, 10, 1648.

Guo, D., Xie, W., Xiong, P., Li, H., Wang, S., Chen, G., ... & Zhang, J. (2018). Cyclin-dependent kinase 5-mediated phosphorylation of chloride intracellular channel 4 promotes oxidative stress-induced neuronal death. Cell death & disease, 9(10), 1-13.

Argenzio, E., Klarenbeek, J., Kedziora, K. M., Nahidiazar, L., Isogai, T., Perrakis, A., ... & Innocenti, M. (2018). Profilin binding couples chloride intracellular channel protein CLIC4 to RhoA–mDia2 signaling and filopodium formation. Journal of Biological Chemistry, 293(50), 19161-19176.

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For research use only. Not intended for any clinical use.

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