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Mouse Anti-CPSF3 Recombinant Antibody (10B2590) (CBMAB-C2204-LY)

This product is antibody recognizes CPSF3. The antibody 10B2590 immunoassay techniques such as: ELISA, IHC-F, WB.
See all CPSF3 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
10B2590
Antibody Isotype
IgG2a
Application
ELISA, IHC-F, WB

Basic Information

Specificity
Human
Antibody Isotype
IgG2a
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Concentration
0.5 mg/ml
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
cleavage and polyadenylation specific factor 3, 73kDa
Introduction
CPSF2 (Cleavage And Polyadenylation Specific Factor 2) is a Protein Coding gene. Among its related pathways are mRNA Splicing - Major Pathway and RNA Polymerase II Transcription Termination. Gene Ontology (GO) annotations related to this gene include RNA binding.
Entrez Gene ID
51692
UniProt ID
Q9UKF6
Alternative Names
Cleavage And Polyadenylation Specific Factor 3; Cleavage And Polyadenylation Specific Factor 3, 73kDa; MRNA 3-End-Processing Endonuclease CPSF-73; CPSF73; Cleavage And Polyadenylation Specificity Factor 73 KDa Subunit; Cleavage And Polyadenylation Specific Factor 3, 73kD Subunit;
Function
Component of the cleavage and polyadenylation specificity factor (CPSF) complex that play a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. Has endonuclease activity, and functions as mRNA 3'-end-processing endonuclease. Also involved in the histone 3'-end pre-mRNA processing. U7 snRNP-dependent protein that induces both the 3'-endoribonucleolytic cleavage of histone pre-mRNAs and acts as a 5' to 3' exonuclease for degrading the subsequent downstream cleavage product (DCP) of mature histone mRNAs. Cleavage occurs after the 5'-ACCCA-3' sequence in the histone pre-mRNA leaving a 3'hydroxyl group on the upstream fragment containing the stem loop (SL) and 5' phosphate on the downstream cleavage product (DCP) starting with CU nucleotides. The U7-dependent 5' to 3' exonuclease activity is processive and degrades the DCP RNA substrate even after complete removal of the U7-binding site. Binds to the downstream cleavage product (DCP) of histone pre-mRNAs and the cleaved DCP RNA substrate in a U7 snRNP dependent manner. Required for the selective processing of microRNAs (miRNAs) during embryonic stem cell differentiation via its interaction with ISY1 (By similarity).

Required for the biogenesis of all miRNAs from the pri-miR-17-92 primary transcript except miR-92a (By similarity).

Only required for the biogenesis of miR-290 and miR-96 from the pri-miR-290-295 and pri-miR-96-183 primary transcripts, respectively (By similarity).
Biological Process
mRNA 3'-end processing Source: Reactome
mRNA 3'-end processing by stem-loop binding and cleavage Source: UniProtKB
mRNA cleavage Source: ProtInc
mRNA export from nucleus Source: Reactome
mRNA polyadenylation Source: GO_Central
mRNA splicing, via spliceosome Source: Reactome
Termination of RNA polymerase II transcription Source: Reactome
Cellular Location
Nucleus
PTM
Sumoylated on Lys-462, Lys-465 and Lys-545, preferentially by SUMO3.

Li, N., Jiang, S., Fu, R., Lv, J., Yao, J., Mai, J., ... & Lu, M. (2021). Cleavage and polyadenylation-specific factor 3 induces cell cycle arrest via PI3K/Akt/GSK-3β signaling pathways and predicts a negative prognosis in hepatocellular carcinoma. Biomarkers in Medicine, 15(5), 347-358.

Ross, N. T., Lohmann, F., Carbonneau, S., Fazal, A., Weihofen, W. A., Gleim, S., ... & Beckwith, R. E. (2020). CPSF3-dependent pre-mRNA processing as a druggable node in AML and Ewing’s sarcoma. Nature chemical biology, 16(1), 50-59.

Kakegawa, J., Sakane, N., Suzuki, K., & Yoshida, T. (2019). JTE-607, a multiple cytokine production inhibitor, targets CPSF3 and inhibits pre-mRNA processing. Biochemical and biophysical research communications, 518(1), 32-37.

Ning, Y., Liu, W., Guan, X., Xie, X., & Zhang, Y. (2019). CPSF3 is a promising prognostic biomarker and predicts recurrence of non‑small cell lung cancer. Oncology Letters, 18(3), 2835-2844.

Swale, C., Bougdour, A., Gnahoui-David, A., Tottey, J., Georgeault, S., Laurent, F., ... & Hakimi, M. A. (2019). Metal-captured inhibition of pre-mRNA processing activity by CPSF3 controls Cryptosporidium infection. Science translational medicine, 11(517), eaax7161.

Wall, R. J., Rico, E., Lukac, I., Zuccotto, F., Elg, S., Gilbert, I. H., ... & Horn, D. (2018). Clinical and veterinary trypanocidal benzoxaboroles target CPSF3. Proceedings of the National Academy of Sciences, 115(38), 9616-9621.

Palencia, A., Bougdour, A., Brenier‐Pinchart, M. P., Touquet, B., Bertini, R. L., Sensi, C., ... & Hakimi, M. A. (2017). Targeting Toxoplasma gondii CPSF 3 as a new approach to control toxoplasmosis. EMBO molecular medicine, 9(3), 385-394.

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For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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