CPSF3

CPSF3 (Cleavage And Polyadenylation Specific Factor 3) is a Protein Coding gene. Among its related pathways are mRNA Splicing - Major Pathway and RNA Polymerase II Transcription Termination. Gene Ontology (GO) annotations related to this gene include RNA binding and 5-3 exonuclease activity. An important paralog of this gene is INTS11.

Full Name

cleavage and polyadenylation specific factor 3, 73kDa

Function

Component of the cleavage and polyadenylation specificity factor (CPSF) complex that play a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. Has endonuclease activity, and functions as mRNA 3'-end-processing endonuclease. Also involved in the histone 3'-end pre-mRNA processing. U7 snRNP-dependent protein that induces both the 3'-endoribonucleolytic cleavage of histone pre-mRNAs and acts as a 5' to 3' exonuclease for degrading the subsequent downstream cleavage product (DCP) of mature histone mRNAs. Cleavage occurs after the 5'-ACCCA-3' sequence in the histone pre-mRNA leaving a 3'hydroxyl group on the upstream fragment containing the stem loop (SL) and 5' phosphate on the downstream cleavage product (DCP) starting with CU nucleotides. The U7-dependent 5' to 3' exonuclease activity is processive and degrades the DCP RNA substrate even after complete removal of the U7-binding site. Binds to the downstream cleavage product (DCP) of histone pre-mRNAs and the cleaved DCP RNA substrate in a U7 snRNP dependent manner. Required for the selective processing of microRNAs (miRNAs) during embryonic stem cell differentiation via its interaction with ISY1 (By similarity).

Required for the biogenesis of all miRNAs from the pri-miR-17-92 primary transcript except miR-92a (By similarity).

Only required for the biogenesis of miR-290 and miR-96 from the pri-miR-290-295 and pri-miR-96-183 primary transcripts, respectively (By similarity).

Biological Process

mRNA 3'-end processing Source: Reactome
mRNA 3'-end processing by stem-loop binding and cleavage Source: UniProtKB
mRNA cleavage Source: ProtInc
mRNA export from nucleus Source: Reactome
mRNA polyadenylation Source: GO_Central
mRNA splicing, via spliceosome Source: Reactome
Termination of RNA polymerase II transcription Source: Reactome

Cellular Location

Nucleus

PTM

Sumoylated on Lys-462, Lys-465 and Lys-545, preferentially by SUMO3.