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Mouse Anti-ATP5F1A Recombinant Antibody (51) (CBMAB-A4043-YC)

Provided herein is a Mouse monoclonal antibody against Human ATP Synthase F1 Subunit Alpha. The antibody can be used for immunoassay techniques, such as WB, IF.
See all ATP5F1A antibodies
Published Data
Mouse Anti-ATP5F1A Recombinant Antibody (51) in WB
Western analysis of protein expression after feeding with the Western diet supplemented with 15% fructose in drinking water (WDF). ...View More
Zhang, S., Guo, Y., Fidelito, G., Robinson, D. R., Liang, C., Lim, R., ... & Ho, L. (2023). LINC00116-encoded microprotein mitoregulin regulates fatty acid metabolism at the mitochondrial outer membrane. Iscience, 26(9).
Mouse Anti-ATP5F1A Recombinant Antibody (51) in WB
ES1 protein level in the brains of WT, heterozygote, and KO mice. ES1 deficiency was confirmed in ES1-KO− /− mice (40 µg proteins/lane). Anti-COX4, anti-Ak2, anti-AIF, and anti-ATP5A antibodies were used as mitochondrial markers. Anti-beta-actin antibody was used as the cytosolic marker. ...View More
Ito, G., Tatara, Y., Itoh, K., Yamada, M., Yamashita, T., Sakamoto, K., ... & Ozaki, T. (2023). Novel dicarbonyl metabolic pathway via mitochondrial ES1 possessing glyoxalase III activity. BBA advances, 3, 100092.
Mouse Anti-ATP5F1A Recombinant Antibody (51) in Immunoblot
Immunoblot analysis of IRG1, ATP5A, SDHA, Tubulin as a loading control. ...View More
Zhang, X., Chen, C., Ling, C., Luo, S., Xiong, Z., Liu, X., ... & Tang, J. (2022). EGFR tyrosine kinase activity and Rab GTPases coordinate EGFR trafficking to regulate macrophage activation in sepsis. Cell Death & Disease, 13(11), 934.
Mouse Anti-ATP5F1A Recombinant Antibody (51) in IF
VCaP cells expressing vector (Vec), FLAG-tagged ATP5F1A and Mut-ATP5F1A were treated with DMSO or oligomycin (Oligo) (0.5 μM) for 3 h co-stained with MitoTracker and TOMM20 and were processed for immunofluoresence-based detection. Bars: 50μm. MitoTracker Red staining intensity was normalized with DAPI staining intensity using densitometric analysis by ImageJ software (NIH) and fold changes were represented as mean ± SEM of relative MitoTracker staining intensity. ***p < 0.001. ...View More
Chouhan, S., Sawant, M., Weimholt, C., Luo, J., Sprung, R. W., Terrado, M., ... & Mahajan, N. P. (2023). TNK2/ACK1-mediated phosphorylation of ATP5F1A (ATP synthase F1 subunit alpha) selectively augments survival of prostate cancer while engendering mitochondrial vulnerability. Autophagy, 19(3), 1000-1025.

Summary

Host Animal
Mouse
Specificity
Human, Arabidopsis thaliana, Dog, Mouse, Rat
Clone
51
Antibody Isotype
IgG2a, κ
Application
WB, IF, IP

Basic Information

Immunogen
Amino acids 113-220 of ATP5A of human origin.
Specificity
Human, Arabidopsis thaliana, Dog, Mouse, Rat
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
ATP Synthase F1 Subunit Alpha
Introduction
ATP5F1A is a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, using an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-s
Entrez Gene ID
Human498
Mouse11946
Rat65262
Dog480149
UniProt ID
HumanP25705
MouseQ03265
RatP15999
DogE2RNG2
Alternative Names
ATP Synthase F1 Subunit Alpha; ATP Synthase, H+ Transporting, Mitochondrial F1 Complex, Alpha Subunit 1, Cardiac Muscle; ATP5A1; ATP Synthase, H+ Transporting, Mitochondrial F1 Complex, Alpha Subunit, Isoform 2, Non-Cardiac Muscle-Like 2; ATP Synthase, H+
Function
Mitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F1 - containing the extramembraneous catalytic core, and F0 - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F1. Rotation of the central stalk against the surrounding alpha3beta3 subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. Subunit alpha does not bear the catalytic high-affinity ATP-binding sites (By similarity).
Binds the bacterial siderophore enterobactin and can promote mitochondrial accumulation of enterobactin-derived iron ions (PubMed:30146159).
Biological Process
ATP biosynthetic process Source: CAFA
ATP synthesis coupled proton transport Source: GO_Central
Cristae formation Source: Reactome
Lipid metabolic process Source: UniProtKB
Mitochondrial ATP synthesis coupled proton transport Source: UniProtKB
Negative regulation of endothelial cell proliferation Source: UniProtKB
Positive regulation of blood vessel endothelial cell migration Source: CAFA
Cellular Location
Mitochondrion; Mitochondrion inner membrane; Cell membrane. Colocalizes with HRG on the cell surface of T-cells (PubMed:19285951).
Involvement in disease
Combined oxidative phosphorylation deficiency 22 (COXPD22): A mitochondrial disorder characterized by intrauterine growth retardation, microcephaly, hypotonia, pulmonary hypertension, failure to thrive, encephalopathy, and heart failure.
Mitochondrial complex V deficiency, nuclear type 4 (MC5DN4): A mitochondrial disorder with heterogeneous clinical manifestations including dysmorphic features, psychomotor retardation, hypotonia, growth retardation, cardiomyopathy, enlarged liver, hypoplastic kidneys and elevated lactate levels in urine, plasma and cerebrospinal fluid.
PTM
The N-terminus is blocked.
Acetylated on lysine residues. BLOC1S1 is required for acetylation.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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